FastQCFastQC Report
Thu 2 Feb 2017
SRR4062353_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062353_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1562250
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG852.3536086E-723.2940855
AGTATCG600.007413108418.3333078
GGCGTTA2450.017.95915642
GTACCGT1408.37681E-817.285696
ACCGGTC1053.775528E-516.761888
CGTCGTA2251.8189894E-1216.62219810
TTAACGG2400.016.49997535
ACGATTA1351.0859585E-616.29627240
ACCGTCG2301.8189894E-1216.2608458
GTACTAA1105.619922E-516.0010011
TACCGTC2201.8189894E-1115.9999777
CGATTAA1401.5868773E-615.71426441
CGGCGTT3100.015.61288141
TACGCTA2403.6379788E-1215.5833119
TAACGGC2700.015.48145936
GTCCTAA1857.565177E-915.4604261
CCTATAC1452.2865297E-615.1723933
CGCAATA3350.015.10445536
ATACCGT2651.8189894E-1214.9433746
GTATTAG5200.014.80861951
Produced by FastQC (version 0.11.5)