FastQCFastQC Report
Thu 2 Feb 2017
SRR4062352_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062352_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences961554
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG110771.151989383851557No Hit
TCCATGTACTCTGCGTTGATACCAC89070.9263130307814225No Hit
GTATCAACGCAGAGTACTTTTTTTT67370.7006366777112882No Hit
GTACTTTTTTTTTTTTTTTTTTTTT58290.6062062037077481No Hit
TATCAACGCAGAGTACTTTTTTTTT51600.5366313280377389No Hit
GAGTACATGGAAGCAGTGGTATCAA41480.43138502881793434No Hit
GTACATGGGAAGCAGTGGTATCAAC40300.41911322713024957No Hit
CCCATGTACTCTGCGTTGATACCAC38100.39623359686507464No Hit
GAGTACTTTTTTTTTTTTTTTTTTT37320.38812172795287625No Hit
CATGTACTCTGCGTTGATACCACTG36070.37512193802948146No Hit
GTACATGGGGTGGTATCAACGCAAA30650.31875484892164146No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28540.2968112035309509No Hit
GCTTCCATGTACTCTGCGTTGATAC26770.27840350099942385No Hit
GCGTTGATACCACTGCTTCCATGTA25760.2678996707413208No Hit
CATGGAAGCAGTGGTATCAACGCAG24670.25656385392812053No Hit
GGTATCAACGCAGAGTACTTTTTTT21910.2278603177772647No Hit
GTATCAACGCAGAGTACATGGGAAG21700.22567635307013437No Hit
GTATCAACGCAGAGTACATGGAAGC19110.19874078834886028No Hit
GTCTACCACCCTGAAATCGATTTAT18900.19655682364172994No Hit
GTACATGGGTGGTATCAACGCAAAA18630.19374886901827668No Hit
GTTCTAAGCTGGTTGTTAAGCGTAC18320.19052492111727473No Hit
ACTCTGCGTTGATACCACTGCTTCC17290.1798130942203974No Hit
GCCCTGGCCGTCCACACGCCGACGC17130.17814912111020284No Hit
CAGTGGTATCAACGCAGAGTACATG16390.1704532454755531No Hit
GTATCAACGCAGAGTACATGGGCAG15540.16161338832764463No Hit
ACGCAGAGTACATGGAAGCAGTGGT15510.16130139336948315No Hit
GCGTTGATACCACTGCTTCCCATGT13940.14497365722569924No Hit
GCAGAGTACTTTTTTTTTTTTTTTT13360.13894175470124404No Hit
GGGTAGTTCCGTATGAAAGTTGCGC13150.1367577899941137No Hit
GAGTACATGGGAAGCAGTGGTATCA12970.13488582024514484No Hit
GGTATAGGTAACACGCTTAAGCGCC12970.13488582024514484No Hit
AGCGTACACCGGCCGTCCCGAGGGA12750.13259785721862735No Hit
GTACATGGGAGTGGTATCAACGCAA12720.13228586226046588No Hit
TATCAACGCAGAGTACATGGAAGCA12390.12885391772068963No Hit
TATCAACGCAGAGTACATGGGAAGC12300.1279179328462052No Hit
GTGGTAGACTGTAAGAAAGAAAAGA12240.12729394292988225No Hit
GTCCAAGCCAGCATCAGCGGCTTCC12180.1266699530135593No Hit
GTATCAACGCAGAGTACATGGGGTG12170.12656595469417214No Hit
GTTTTAATTAGACAGTCAGATTCCC11880.12355000343194453No Hit
GTACATGGGCAGAAATCACATTGCG11420.11876608074013525No Hit
CCCTTAGAGCCAATCCTTATCCCGA11320.11772609754626365No Hit
GTTCTATGGCTGGTAGAGCTCGGCA11140.1158541277972948No Hit
GAATAATGTGGATAAGGGAATTCGG11050.11491814292281036No Hit
GTCCGTACCAGTTCTAAGCTGGTTG10820.11252618157690572No Hit
GGCTTGGACTGCTGCGTGGCAACAT10780.11211018829935708No Hit
TCGCAATGCTATGTTTTAATTAGAC10650.11075821014732401No Hit
GGTATCAACGCAGAGTACATGGGAA10420.10836624880141937No Hit
CCCTTATCCACATTATTCTATCAAC10340.1075342622463221No Hit
GGATAAGGGAATTCGGCAAATTGGA10260.10670227569122483No Hit
GGCTGGTAGAGCTCGGCACTTCTGC9800.10191835299941553No Hit
AGATAGGGTAGTTCCGTATGAAAGT9760.10150235972186689No Hit
CCCCTTGTCCGTACCAGTTCTAAGC9710.10098236812493111No Hit
GCTTCCCATGTACTCTGCGTTGATA9630.10015038156983383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACA607.678864E-817.4160944
CGGCTTA350.002166092916.2894218
AGGACAG350.00216835516.2868755
GTCCTAG350.002182729816.2707731
GTGCTAA350.002182729816.2707731
GTATAAC350.002182729816.2707731
GATATGG1900.014.9885792
CCTTATA456.79815E-414.7665252
TATAACG456.79815E-414.7665252
TAGAGTC400.005274662714.2510165
TGGAGCG602.5645195E-514.2510165
TGCACTG400.005274662714.2510165
CCCTTAT2150.014.1265621
CGATTTA2700.014.07727718
CGAAATT1152.5465852E-1114.04447913
GCGAAAT1152.5465852E-1114.04374912
GGATATG2250.013.920551
TAGGACC1301.8189894E-1213.8841594
GCACCGA551.9568748E-413.8198866
TTAGGAC1450.013.7531553
Produced by FastQC (version 0.11.5)