##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062352_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	961554
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.767055204387898	32.0	32.0	32.0	32.0	32.0
2	30.571411485990385	32.0	32.0	32.0	32.0	32.0
3	30.614632147544494	32.0	32.0	32.0	32.0	32.0
4	30.55523038747694	32.0	32.0	32.0	32.0	32.0
5	30.4544362563101	32.0	32.0	32.0	32.0	32.0
6	34.02185004690324	36.0	36.0	36.0	32.0	36.0
7	33.78605673732312	36.0	36.0	36.0	32.0	36.0
8	33.736867612219385	36.0	36.0	36.0	27.0	36.0
9	33.864590028225145	36.0	36.0	36.0	32.0	36.0
10	33.60908487718839	36.0	36.0	36.0	27.0	36.0
11	34.02294618918958	36.0	36.0	36.0	32.0	36.0
12	33.822392710133805	36.0	36.0	36.0	32.0	36.0
13	33.90761933287158	36.0	36.0	36.0	32.0	36.0
14	33.84841932954363	36.0	36.0	36.0	32.0	36.0
15	33.803533654896135	36.0	36.0	36.0	32.0	36.0
16	33.77323998444185	36.0	36.0	36.0	32.0	36.0
17	33.69763840616336	36.0	36.0	36.0	27.0	36.0
18	33.73835062825385	36.0	36.0	36.0	32.0	36.0
19	33.72036723886542	36.0	36.0	36.0	27.0	36.0
20	33.622193865347135	36.0	36.0	36.0	27.0	36.0
21	33.560184867412545	36.0	36.0	36.0	27.0	36.0
22	33.52559294641799	36.0	36.0	36.0	27.0	36.0
23	33.495135998602265	36.0	36.0	36.0	27.0	36.0
24	33.4632085145504	36.0	36.0	36.0	27.0	36.0
25	32.94172246176502	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	38.0
5	90.0
6	158.0
7	50.0
8	140.0
9	187.0
10	104.0
11	37.0
12	59.0
13	51.0
14	416.0
15	460.0
16	667.0
17	946.0
18	1354.0
19	1956.0
20	2978.0
21	3916.0
22	5492.0
23	7213.0
24	9129.0
25	11394.0
26	14827.0
27	17774.0
28	22831.0
29	29562.0
30	36877.0
31	49109.0
32	69342.0
33	96818.0
34	206019.0
35	371556.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.09770059949993	17.4611115502544	11.80663319008017	22.634554660165495
2	16.376623336103243	19.951888826372326	38.300799876862015	25.37068796066241
3	19.496645377214357	27.769266850336294	27.088253984102074	25.64583378834728
4	12.317823071514455	15.826458143511527	35.69201229837284	36.163706486601185
5	13.280104031209364	38.70273081924577	32.93867360208063	15.07849154746424
6	30.518753576444883	35.9342454351558	18.81537741247464	14.731623575924674
7	29.278465955744082	31.079985555374012	21.273562275798696	18.36798621308321
8	29.974133848227623	31.06363489692573	19.30364364041365	19.658587614433003
9	28.673562756534437	14.251733998444788	18.727703689515646	38.34699955550512
10	17.161015846175793	27.206610434382423	29.97768686857118	25.654686850870608
11	35.667573541737596	23.34625448101445	20.842300568176494	20.14387140907146
12	23.904905212406476	24.967171888098054	29.307147208438984	21.820775691056486
13	29.626883931592786	21.791810917041936	26.025769841476432	22.555535309888853
14	24.44899586935629	19.817718556034418	26.211001235965238	29.522284338644063
15	27.757094192215753	25.221238016707897	24.6342545163789	22.38741327469745
16	24.747530483742004	27.03964135794243	25.191356276652456	23.021471881663114
17	22.69474376902196	25.270953964117147	27.12278074933127	24.911521517529618
18	23.783813615622833	25.845171179313947	28.05803789915634	22.312977305906877
19	24.831774474705252	27.187348357523238	26.26109267971651	21.719784488054998
20	25.469594045839045	23.721371422859548	28.574745627881597	22.23428890341981
21	25.68050800156616	24.35187730654204	26.109014570264666	23.858600121627138
22	24.189726695822166	25.802409859405774	26.067475646755312	23.940387798016747
23	23.318204918852267	26.081830906121905	26.30417485919952	24.29578931582631
24	22.99913569576491	28.06427091251783	25.29433203861253	23.642261353104725
25	24.96147197867422	25.505550117666658	24.337005643834473	25.195972259824646
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	445.0
1	445.0
2	1017.0
3	1589.0
4	1589.0
5	1589.0
6	4327.0
7	7065.0
8	7065.0
9	7065.0
10	6350.0
11	5635.0
12	5635.0
13	5635.0
14	4128.5
15	2622.0
16	2622.0
17	2622.0
18	4225.5
19	5829.0
20	5829.0
21	5829.0
22	8688.5
23	11548.0
24	11548.0
25	11548.0
26	18106.0
27	24664.0
28	24664.0
29	24664.0
30	31224.5
31	37785.0
32	37785.0
33	37785.0
34	42594.5
35	47404.0
36	47404.0
37	47404.0
38	60171.5
39	72939.0
40	72939.0
41	72939.0
42	94725.5
43	116512.0
44	116512.0
45	116512.0
46	152983.5
47	189455.0
48	189455.0
49	189455.0
50	168617.5
51	147780.0
52	147780.0
53	147780.0
54	125815.0
55	103850.0
56	103850.0
57	103850.0
58	88908.0
59	73966.0
60	73966.0
61	73966.0
62	61174.5
63	48383.0
64	48383.0
65	48383.0
66	38544.5
67	28706.0
68	28706.0
69	28706.0
70	22703.5
71	16701.0
72	16701.0
73	16701.0
74	13276.5
75	9852.0
76	9852.0
77	9852.0
78	8035.0
79	6218.0
80	6218.0
81	6218.0
82	3960.0
83	1702.0
84	1702.0
85	1702.0
86	991.0
87	280.0
88	280.0
89	280.0
90	183.0
91	86.0
92	86.0
93	86.0
94	64.5
95	43.0
96	43.0
97	43.0
98	269.0
99	495.0
100	495.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008943855467295649
2	0.00322394790100192
3	0.004679924372422142
4	0.013103788242781998
5	0.03161548909369625
6	0.042015321032412116
7	0.06687091936594305
8	0.08725458996582615
9	0.0949504656004759
10	0.11783009586565081
11	0.11575012947790764
12	0.12989390091456124
13	0.11741410258810218
14	0.12178203200236283
15	0.10919823535651663
16	0.10836624880141937
17	0.11970206561461967
18	0.126461956374785
19	0.12854192276252815
20	0.11959806729523251
21	0.1291659126788511
22	0.14715762193282955
23	0.13831776478492105
24	0.1293739093176254
25	0.12625395973601067
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	961554.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.04633268942675
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.62352798142813	21.520243501204554
2	13.989779843007504	8.406831588296797
3	5.391399050180393	4.8597530856953846
4	2.584288669542873	3.1059358852240484
5	1.4237281673339328	2.1388905087511594
6	0.8888986222948912	1.6024886239587237
7	0.6124689898707852	1.2881712922130335
8	0.4410535216036796	1.0601632675157941
9	0.3215164464521867	0.8694351103702197
>10	2.0739702528087522	12.728677104149916
>50	0.3149610724265257	6.647247478012338
>100	0.2875278433703993	17.36021953229832
>500	0.02986400305537931	6.095822736154048
>1k	0.015279257377170808	8.381547586916179
>5k	0.001389023397924619	2.7788245743528472
>10k+	3.472558494811547E-4	1.1557481248866628
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	11077	1.151989383851557	No Hit
TCCATGTACTCTGCGTTGATACCAC	8907	0.9263130307814225	No Hit
GTATCAACGCAGAGTACTTTTTTTT	6737	0.7006366777112882	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	5829	0.6062062037077481	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5160	0.5366313280377389	No Hit
GAGTACATGGAAGCAGTGGTATCAA	4148	0.43138502881793434	No Hit
GTACATGGGAAGCAGTGGTATCAAC	4030	0.41911322713024957	No Hit
CCCATGTACTCTGCGTTGATACCAC	3810	0.39623359686507464	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	3732	0.38812172795287625	No Hit
CATGTACTCTGCGTTGATACCACTG	3607	0.37512193802948146	No Hit
GTACATGGGGTGGTATCAACGCAAA	3065	0.31875484892164146	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2854	0.2968112035309509	No Hit
GCTTCCATGTACTCTGCGTTGATAC	2677	0.27840350099942385	No Hit
GCGTTGATACCACTGCTTCCATGTA	2576	0.2678996707413208	No Hit
CATGGAAGCAGTGGTATCAACGCAG	2467	0.25656385392812053	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2191	0.2278603177772647	No Hit
GTATCAACGCAGAGTACATGGGAAG	2170	0.22567635307013437	No Hit
GTATCAACGCAGAGTACATGGAAGC	1911	0.19874078834886028	No Hit
GTCTACCACCCTGAAATCGATTTAT	1890	0.19655682364172994	No Hit
GTACATGGGTGGTATCAACGCAAAA	1863	0.19374886901827668	No Hit
GTTCTAAGCTGGTTGTTAAGCGTAC	1832	0.19052492111727473	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1729	0.1798130942203974	No Hit
GCCCTGGCCGTCCACACGCCGACGC	1713	0.17814912111020284	No Hit
CAGTGGTATCAACGCAGAGTACATG	1639	0.1704532454755531	No Hit
GTATCAACGCAGAGTACATGGGCAG	1554	0.16161338832764463	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1551	0.16130139336948315	No Hit
GCGTTGATACCACTGCTTCCCATGT	1394	0.14497365722569924	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	1336	0.13894175470124404	No Hit
GGGTAGTTCCGTATGAAAGTTGCGC	1315	0.1367577899941137	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1297	0.13488582024514484	No Hit
GGTATAGGTAACACGCTTAAGCGCC	1297	0.13488582024514484	No Hit
AGCGTACACCGGCCGTCCCGAGGGA	1275	0.13259785721862735	No Hit
GTACATGGGAGTGGTATCAACGCAA	1272	0.13228586226046588	No Hit
TATCAACGCAGAGTACATGGAAGCA	1239	0.12885391772068963	No Hit
TATCAACGCAGAGTACATGGGAAGC	1230	0.1279179328462052	No Hit
GTGGTAGACTGTAAGAAAGAAAAGA	1224	0.12729394292988225	No Hit
GTCCAAGCCAGCATCAGCGGCTTCC	1218	0.1266699530135593	No Hit
GTATCAACGCAGAGTACATGGGGTG	1217	0.12656595469417214	No Hit
GTTTTAATTAGACAGTCAGATTCCC	1188	0.12355000343194453	No Hit
GTACATGGGCAGAAATCACATTGCG	1142	0.11876608074013525	No Hit
CCCTTAGAGCCAATCCTTATCCCGA	1132	0.11772609754626365	No Hit
GTTCTATGGCTGGTAGAGCTCGGCA	1114	0.1158541277972948	No Hit
GAATAATGTGGATAAGGGAATTCGG	1105	0.11491814292281036	No Hit
GTCCGTACCAGTTCTAAGCTGGTTG	1082	0.11252618157690572	No Hit
GGCTTGGACTGCTGCGTGGCAACAT	1078	0.11211018829935708	No Hit
TCGCAATGCTATGTTTTAATTAGAC	1065	0.11075821014732401	No Hit
GGTATCAACGCAGAGTACATGGGAA	1042	0.10836624880141937	No Hit
CCCTTATCCACATTATTCTATCAAC	1034	0.1075342622463221	No Hit
GGATAAGGGAATTCGGCAAATTGGA	1026	0.10670227569122483	No Hit
GGCTGGTAGAGCTCGGCACTTCTGC	980	0.10191835299941553	No Hit
AGATAGGGTAGTTCCGTATGAAAGT	976	0.10150235972186689	No Hit
CCCCTTGTCCGTACCAGTTCTAAGC	971	0.10098236812493111	No Hit
GCTTCCCATGTACTCTGCGTTGATA	963	0.10015038156983383	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.039983193871587E-4	0.0
4	0.0	0.0	0.0	1.039983193871587E-4	0.0
5	0.0	1.039983193871587E-4	0.0	1.039983193871587E-4	0.0
6	0.0	1.039983193871587E-4	0.0	1.039983193871587E-4	0.0
7	0.0	1.039983193871587E-4	0.0	1.039983193871587E-4	0.0
8	0.0	1.039983193871587E-4	0.0	1.039983193871587E-4	0.0
9	0.0	1.039983193871587E-4	0.0	2.079966387743174E-4	0.0
10	0.0	2.079966387743174E-4	0.0	2.079966387743174E-4	0.0
11	0.0	2.079966387743174E-4	0.0	3.119949581614761E-4	0.0
12	0.0	2.079966387743174E-4	0.0	3.119949581614761E-4	0.0
13	0.0	2.079966387743174E-4	0.0	3.119949581614761E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACA	60	7.678864E-8	17.416094	4
CGGCTTA	35	0.0021660929	16.28942	18
AGGACAG	35	0.002168355	16.286875	5
GTCCTAG	35	0.0021827298	16.270773	1
GTGCTAA	35	0.0021827298	16.270773	1
GTATAAC	35	0.0021827298	16.270773	1
GATATGG	190	0.0	14.988579	2
CCTTATA	45	6.79815E-4	14.766525	2
TATAACG	45	6.79815E-4	14.766525	2
TAGAGTC	40	0.0052746627	14.251016	5
TGGAGCG	60	2.5645195E-5	14.251016	5
TGCACTG	40	0.0052746627	14.251016	5
CCCTTAT	215	0.0	14.126562	1
CGATTTA	270	0.0	14.077277	18
CGAAATT	115	2.5465852E-11	14.044479	13
GCGAAAT	115	2.5465852E-11	14.043749	12
GGATATG	225	0.0	13.92055	1
TAGGACC	130	1.8189894E-12	13.884159	4
GCACCGA	55	1.9568748E-4	13.819886	6
TTAGGAC	145	0.0	13.753155	3
>>END_MODULE