FastQCFastQC Report
Thu 2 Feb 2017
SRR4062352_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062352_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences961554
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA43010.4472967716841696No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC38200.39727358005894625No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA22390.23285223710784836No Hit
GTCTACCACCCTGAAATCGATTTATTCGGAGCTAGGGTTCTATGGCTGGT18210.189380939604016No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17300.17991709253978455No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA16180.1682692807684228No Hit
GTTCTAAGCTGGTTGTTAAGCGTACACCGGCCGTCCCGAGGGACTGCCCT16120.16764529085209984No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA15810.16442134295109792No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT14560.15142155302770308No Hit
GGTATAGGTAACACGCTTAAGCGCCATCCATTTTCAGGGCTAGTTCATTC13750.1429976891573432No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC13080.13602980175840357No Hit
GGGTAGTTCCGTATGAAAGTTGCGCGATTCTTCGCGCCGCCTATCGAAAG13060.13582180511962927No Hit
AGCGTACACCGGCCGTCCCGAGGGACTGCCCTGGCCGTCCACACGCCGAC12620.1312458790665943No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11840.1231340101543959No Hit
GCCCTGGCCGTCCACACGCCGACGCCGGCTCGGTCCACCGCATGTTGCCA11530.11991006225339398No Hit
GTCCAAGCCAGCATCAGCGGCTTCCAACCAGGGCCCGATGTACCCAACCC11060.11502214124219753No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCA10630.1105502135085497No Hit
GGCTTGGACTGCTGCGTGGCAACATGCGGTGGACCGAGCCGGCGTCGGCG10580.1100302219116139No Hit
GTTCTATGGCTGGTAGAGCTCGGCACTTCTGCCGAGTCCGGTGCGTCCTC10570.10992622359222676No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10260.10670227569122483No Hit
CCCTTATCCACATTATTCTATCAACTAGAGGCTGTTAACCTAGGAGACCT10220.1062862824136762No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG10160.10566229249735323No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCA9850.10243834459635133No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA9750.10139836140247974No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC9710.10098236812493111No Hit
GGATAAGGGAATTCGGCAAATTGGATCCGTAACTTCGGGATAAGGATTGG9700.10087836980554395No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGCTGG9640.10025437988922098No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACA450.001397659724.4454844
TATTAGA803.611307E-622.002082
GTATTAG1057.725794E-820.9565411
GTGTAAA1057.725794E-820.9565411
TATAAGG956.8570625E-720.8440782
GCTCTAG654.9245555E-420.3117261
GTCCTAT550.00447780820.0039711
GAATAGT550.00447780820.0039711
GTCATAA1900.019.6881221
ATACATG3600.019.5594391
TCGGAGC5850.019.5553726
GTTATGG808.972441E-519.2538221
GGAGCTA5950.019.2267128
CGATTTA5650.019.07946618
GGATATG3000.019.0704541
CTTAAGA708.116513E-418.8589252
AATATAG708.122191E-418.8569625
CCTACAC1303.5832272E-818.6161773
CTAGGGT5950.018.4872232
GTATAGA600.00740254318.3369731
Produced by FastQC (version 0.11.5)