##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062352_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	961554
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.99879153952872	32.0	32.0	32.0	32.0	32.0
2	31.359580429180266	32.0	32.0	32.0	32.0	32.0
3	31.483777302158796	32.0	32.0	32.0	32.0	32.0
4	31.605832849741148	32.0	32.0	32.0	32.0	32.0
5	31.525665745241557	32.0	32.0	32.0	32.0	32.0
6	35.11732674399982	36.0	36.0	36.0	36.0	36.0
7	35.133238486866055	36.0	36.0	36.0	36.0	36.0
8	35.06925040091352	36.0	36.0	36.0	36.0	36.0
9	35.15222857998615	36.0	36.0	36.0	36.0	36.0
10	35.077460028245945	36.0	36.0	36.0	36.0	36.0
11	35.181157792490076	36.0	36.0	36.0	36.0	36.0
12	35.10866472397806	36.0	36.0	36.0	36.0	36.0
13	35.109755666348434	36.0	36.0	36.0	36.0	36.0
14	35.0761777289679	36.0	36.0	36.0	36.0	36.0
15	35.066239649567265	36.0	36.0	36.0	36.0	36.0
16	35.05763898855395	36.0	36.0	36.0	36.0	36.0
17	35.04137677135137	36.0	36.0	36.0	36.0	36.0
18	35.0363994117855	36.0	36.0	36.0	36.0	36.0
19	35.00628565842376	36.0	36.0	36.0	36.0	36.0
20	34.95428025883102	36.0	36.0	36.0	36.0	36.0
21	34.89632095545336	36.0	36.0	36.0	32.0	36.0
22	34.87891475673753	36.0	36.0	36.0	32.0	36.0
23	34.8447773083987	36.0	36.0	36.0	32.0	36.0
24	34.82014010653588	36.0	36.0	36.0	32.0	36.0
25	34.782070481741016	36.0	36.0	36.0	32.0	36.0
26	34.72204889168991	36.0	36.0	36.0	32.0	36.0
27	34.686436747182164	36.0	36.0	36.0	32.0	36.0
28	34.634852540782944	36.0	36.0	36.0	32.0	36.0
29	34.61168899510584	36.0	36.0	36.0	32.0	36.0
30	34.57629004715284	36.0	36.0	36.0	32.0	36.0
31	34.51983975938949	36.0	36.0	36.0	32.0	36.0
32	34.45957065333824	36.0	36.0	36.0	32.0	36.0
33	34.374308671171875	36.0	36.0	36.0	32.0	36.0
34	34.336033129704624	36.0	36.0	36.0	32.0	36.0
35	34.242323364054435	36.0	36.0	36.0	32.0	36.0
36	34.17470157682252	36.0	36.0	36.0	32.0	36.0
37	34.06764674682857	36.0	36.0	36.0	32.0	36.0
38	34.00708332553346	36.0	36.0	36.0	32.0	36.0
39	33.86330564898071	36.0	36.0	36.0	32.0	36.0
40	33.72984980562714	36.0	36.0	36.0	32.0	36.0
41	33.7126838430291	36.0	36.0	36.0	27.0	36.0
42	33.52804626677233	36.0	36.0	36.0	21.0	36.0
43	33.513654979335534	36.0	36.0	36.0	21.0	36.0
44	33.50177213136236	36.0	36.0	36.0	21.0	36.0
45	33.26677440892555	36.0	36.0	36.0	14.0	36.0
46	33.3269239169095	36.0	36.0	36.0	14.0	36.0
47	33.12417503333146	36.0	36.0	36.0	14.0	36.0
48	33.12429255143237	36.0	36.0	36.0	14.0	36.0
49	33.09958775066195	36.0	36.0	36.0	14.0	36.0
50	32.57662596172446	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	9.0
21	33.0
22	129.0
23	385.0
24	1157.0
25	2921.0
26	6185.0
27	12019.0
28	19184.0
29	26042.0
30	33528.0
31	43092.0
32	60865.0
33	79872.0
34	153224.0
35	522906.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.58629817818713	18.054201923226945	12.347033768287039	23.012466130298886
2	16.19945211406178	20.341357484134395	37.72092762039866	25.738262781405158
3	19.316600557851626	27.47242461619101	27.491352467796652	25.71962235816071
4	12.28047186264691	15.954639951495034	35.972818914441355	35.7920692714167
5	13.152459456255187	38.57453663548797	33.03589813988606	15.237105768370785
6	30.86026996080275	36.09423148322443	18.277421696495335	14.768076859477489
7	28.98605587633326	30.76619881192073	21.530608849027406	18.717136462718607
8	29.957547886026166	30.979331374005	19.43406194555896	19.629058794409882
9	28.63728922140618	13.706666500269355	18.820055867897175	38.835988410427284
10	17.0268128467044	26.92734885404252	30.335893771956645	25.70994452729644
11	35.89855587933699	22.884830181144274	20.96148526239816	20.255128677120577
12	23.6005466151667	24.926629185672358	29.60468158834553	21.868142610815408
13	30.07506598693365	21.320695457561406	26.237216006589332	22.36702254891561
14	24.39332580385501	19.482213167435212	26.54515503029471	29.579305998415066
15	27.983867780696663	24.96105262938951	24.83178271839127	22.223296871522553
16	25.045395266412495	26.365653931032472	25.403565478381868	23.18538532417316
17	22.699193181038194	24.92257325121626	27.57255442752045	24.805679140225095
18	24.053771291055938	24.915813360456095	28.75002339962186	22.280391948866107
19	24.998388026049497	26.544011048781453	26.897813331336568	21.559787593832482
20	25.534707359129076	23.051955480399435	29.358205571397967	22.055131589073522
21	25.728352229827966	23.709744850523215	26.759391568232253	23.802511351416562
22	24.18356119365111	25.024595602535065	26.82532650272372	23.96651670109011
23	23.294687557849063	25.07909072189393	27.199200460920554	24.42702125933645
24	23.05736339300757	27.18141674830535	26.02755539470482	23.73366446398226
25	25.22666433710431	24.40622159545902	25.169777256399538	25.197336811037136
26	22.82679911892624	24.436485106400678	27.338870203857507	25.397845570815576
27	25.094794468121396	25.68311296089455	26.82595049264004	22.396142078344013
28	24.128442084375916	24.214552692828484	27.41915690642439	24.237848316371206
29	23.389950018407703	25.279599481672378	27.729799886433838	23.600650613486085
30	24.847486464618733	24.919037308357098	27.65242513680979	22.581051090214384
31	23.39837388227806	24.687433051081893	26.62419375302895	25.289999313611094
32	22.879630265174917	25.674169105427257	27.044242965033682	24.401957664364144
33	23.568203137837294	24.892725733552147	26.976228064154483	24.56284306445608
34	22.65832184151904	25.42176518427462	28.43054056246451	23.489372411741826
35	23.789615559812553	24.857990294876835	27.204088381931747	24.148305763378865
36	24.99298011344137	25.513075708696547	26.062290833380132	23.431653344481955
37	25.111226202584568	26.006859729146775	25.47002040447026	23.411893663798395
38	24.576466844295798	25.992923954348896	25.612394103711285	23.81821509764402
39	24.271231776894485	24.26519987437003	27.275223232392566	24.18834511634292
40	24.239512289481404	25.75996771892166	25.542715229721885	24.45780476187505
41	23.023147945929193	25.954028582898097	27.305694739973003	23.717128731199704
42	24.776663609116074	27.045386946546945	25.640889643223364	22.537059801113614
43	24.508763938374756	25.039571360526814	24.976652377297583	25.47501232380085
44	23.795959457295172	24.510531909804335	28.45622814735314	23.237280485547355
45	26.04700308043022	24.478396429113705	25.782327357589903	23.692273132866173
46	24.34863184868645	24.708986400125628	26.57565417610886	24.366727575079064
47	23.925437364932183	24.963652587374188	26.603706084109678	24.507203963583947
48	23.185905315770096	27.900565126867548	24.628362005669988	24.285167551692368
49	23.24071243008713	25.48520415910079	27.696936417507494	23.577146993304588
50	23.01599599814051	27.495936261443727	25.470878880311332	24.017188860104437
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	26.0
1	31.5
2	37.0
3	145.5
4	254.0
5	316.0
6	378.0
7	405.5
8	433.0
9	588.0
10	743.0
11	1099.5
12	1456.0
13	2791.0
14	4126.0
15	5685.5
16	7245.0
17	8442.5
18	9640.0
19	10260.0
20	10880.0
21	11043.5
22	11207.0
23	10872.5
24	10538.0
25	8723.5
26	6909.0
27	6388.0
28	5867.0
29	7105.0
30	8343.0
31	9957.0
32	11571.0
33	12635.5
34	13700.0
35	15089.0
36	16478.0
37	20787.5
38	25097.0
39	28922.5
40	32748.0
41	38558.0
42	44368.0
43	52277.5
44	60187.0
45	69917.5
46	79648.0
47	88500.5
48	97353.0
49	98328.0
50	99303.0
51	93806.0
52	88309.0
53	80326.0
54	72343.0
55	66011.5
56	59680.0
57	51429.0
58	43178.0
59	40659.5
60	38141.0
61	33386.0
62	28631.0
63	26169.5
64	23708.0
65	18995.0
66	14282.0
67	11349.0
68	8416.0
69	7597.0
70	6778.0
71	6247.5
72	5717.0
73	6277.0
74	6837.0
75	5247.5
76	3658.0
77	2969.5
78	2281.0
79	1448.0
80	615.0
81	432.5
82	250.0
83	184.0
84	118.0
85	78.0
86	38.0
87	27.5
88	17.0
89	11.5
90	6.0
91	6.0
92	6.0
93	3.5
94	1.0
95	2.0
96	3.0
97	4.5
98	6.0
99	3.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015495749588686646
2	0.004159932775486349
3	8.319865550972696E-4
4	3.119949581614761E-4
5	0.0
6	7.279882357101109E-4
7	2.079966387743174E-4
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.039983193871587E-4
47	0.0
48	0.0
49	0.0
50	1.039983193871587E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	961554.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.55042354081778
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.56967618682381	27.625725005760497
2	13.544262705703138	10.171856022945109
3	4.961298944914529	5.5889663008245885
4	2.3482966868637827	3.5271814076493673
5	1.273392267550949	2.3908209490070242
6	0.8162614278707813	1.839057740194831
7	0.5231113055997846	1.3750135758983453
8	0.3930236975275672	1.1806565042990724
9	0.2756267748303883	0.9314911920663624
>10	1.7921970160234588	13.390727477362832
>50	0.256859259067381	6.706112543341426
>100	0.22036357670986093	16.571459628019376
>500	0.01950120147854435	5.0276141008663195
>1k	0.006128949036113938	3.6733175517648755
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	4301	0.4472967716841696	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	3820	0.39727358005894625	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2239	0.23285223710784836	No Hit
GTCTACCACCCTGAAATCGATTTATTCGGAGCTAGGGTTCTATGGCTGGT	1821	0.189380939604016	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1730	0.17991709253978455	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	1618	0.1682692807684228	No Hit
GTTCTAAGCTGGTTGTTAAGCGTACACCGGCCGTCCCGAGGGACTGCCCT	1612	0.16764529085209984	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	1581	0.16442134295109792	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	1456	0.15142155302770308	No Hit
GGTATAGGTAACACGCTTAAGCGCCATCCATTTTCAGGGCTAGTTCATTC	1375	0.1429976891573432	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	1308	0.13602980175840357	No Hit
GGGTAGTTCCGTATGAAAGTTGCGCGATTCTTCGCGCCGCCTATCGAAAG	1306	0.13582180511962927	No Hit
AGCGTACACCGGCCGTCCCGAGGGACTGCCCTGGCCGTCCACACGCCGAC	1262	0.1312458790665943	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1184	0.1231340101543959	No Hit
GCCCTGGCCGTCCACACGCCGACGCCGGCTCGGTCCACCGCATGTTGCCA	1153	0.11991006225339398	No Hit
GTCCAAGCCAGCATCAGCGGCTTCCAACCAGGGCCCGATGTACCCAACCC	1106	0.11502214124219753	No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCA	1063	0.1105502135085497	No Hit
GGCTTGGACTGCTGCGTGGCAACATGCGGTGGACCGAGCCGGCGTCGGCG	1058	0.1100302219116139	No Hit
GTTCTATGGCTGGTAGAGCTCGGCACTTCTGCCGAGTCCGGTGCGTCCTC	1057	0.10992622359222676	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1026	0.10670227569122483	No Hit
CCCTTATCCACATTATTCTATCAACTAGAGGCTGTTAACCTAGGAGACCT	1022	0.1062862824136762	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	1016	0.10566229249735323	No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCA	985	0.10243834459635133	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	975	0.10139836140247974	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	971	0.10098236812493111	No Hit
GGATAAGGGAATTCGGCAAATTGGATCCGTAACTTCGGGATAAGGATTGG	970	0.10087836980554395	No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGCTGG	964	0.10025437988922098	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.039983193871587E-4	0.0
4	0.0	0.0	0.0	1.039983193871587E-4	0.0
5	0.0	0.0	0.0	1.039983193871587E-4	0.0
6	0.0	0.0	0.0	1.039983193871587E-4	0.0
7	0.0	0.0	0.0	1.039983193871587E-4	0.0
8	0.0	0.0	0.0	1.039983193871587E-4	0.0
9	0.0	0.0	0.0	2.079966387743174E-4	0.0
10	0.0	0.0	0.0	2.079966387743174E-4	0.0
11	0.0	0.0	0.0	3.119949581614761E-4	0.0
12	0.0	0.0	0.0	3.119949581614761E-4	0.0
13	0.0	0.0	0.0	3.119949581614761E-4	0.0
14	0.0	0.0	0.0	5.199915969357935E-4	0.0
15	0.0	0.0	0.0	5.199915969357935E-4	0.0
16	0.0	0.0	0.0	6.239899163229522E-4	0.0
17	0.0	0.0	0.0	6.239899163229522E-4	0.0
18	0.0	0.0	0.0	0.001039983193871587	0.0
19	0.0	0.0	0.0	0.0012479798326459045	0.0
20	0.0	0.0	0.0	0.002079966387743174	0.0
21	0.0	0.0	0.0	0.0030159512622276023	0.0
22	1.039983193871587E-4	0.0	0.0	0.004263931094873507	0.0
23	1.039983193871587E-4	0.0	0.0	0.010295833619328712	0.0
24	1.039983193871587E-4	0.0	0.0	0.01840770253152709	0.0
25	1.039983193871587E-4	0.0	0.0	0.022879630265174915	0.0
26	1.039983193871587E-4	0.0	0.0	0.028391541192694325	0.0
27	1.039983193871587E-4	2.079966387743174E-4	0.0	0.036295413466118386	0.0
28	2.079966387743174E-4	2.079966387743174E-4	0.0	0.05231115465174083	0.0
29	2.079966387743174E-4	2.079966387743174E-4	0.0	0.07186283869652667	0.0
30	2.079966387743174E-4	2.079966387743174E-4	0.0	0.10285433787389996	0.0
31	2.079966387743174E-4	3.119949581614761E-4	0.0	0.1621333799245804	0.0
32	2.079966387743174E-4	3.119949581614761E-4	0.0	0.22775631945787755	0.0
33	2.079966387743174E-4	3.119949581614761E-4	0.0	0.2863073732728479	0.0
34	2.079966387743174E-4	3.119949581614761E-4	0.0	0.36056217331527923	0.0
35	2.079966387743174E-4	3.119949581614761E-4	0.0	0.4512487078208816	0.0
36	3.119949581614761E-4	3.119949581614761E-4	0.0	0.5700147885610168	0.0
37	3.119949581614761E-4	3.119949581614761E-4	0.0	0.737244086135568	0.0
38	3.119949581614761E-4	3.119949581614761E-4	0.0	0.9287049921273273	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACA	45	0.0013976597	24.445484	4
TATTAGA	80	3.611307E-6	22.00208	2
GTATTAG	105	7.725794E-8	20.956541	1
GTGTAAA	105	7.725794E-8	20.956541	1
TATAAGG	95	6.8570625E-7	20.844078	2
GCTCTAG	65	4.9245555E-4	20.311726	1
GTCCTAT	55	0.004477808	20.003971	1
GAATAGT	55	0.004477808	20.003971	1
GTCATAA	190	0.0	19.688122	1
ATACATG	360	0.0	19.559439	1
TCGGAGC	585	0.0	19.55537	26
GTTATGG	80	8.972441E-5	19.253822	1
GGAGCTA	595	0.0	19.22671	28
CGATTTA	565	0.0	19.079466	18
GGATATG	300	0.0	19.070454	1
CTTAAGA	70	8.116513E-4	18.858925	2
AATATAG	70	8.122191E-4	18.856962	5
CCTACAC	130	3.5832272E-8	18.616177	3
CTAGGGT	595	0.0	18.48722	32
GTATAGA	60	0.007402543	18.336973	1
>>END_MODULE