FastQCFastQC Report
Thu 2 Feb 2017
SRR4062351_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062351_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences589051
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG353335.998292168250288No Hit
TCCATGTACTCTGCGTTGATACCAC299235.079865750164247No Hit
GAGTACATGGAAGCAGTGGTATCAA130512.215597630765418No Hit
CATGTACTCTGCGTTGATACCACTG113301.92343277576984No Hit
CCCATGTACTCTGCGTTGATACCAC85151.4455454621076953No Hit
GCGTTGATACCACTGCTTCCATGTA84691.437736291085152No Hit
GCTTCCATGTACTCTGCGTTGATAC84591.4360386452106864No Hit
GTATCAACGCAGAGTACTTTTTTTT82951.408197252869446No Hit
GTACTTTTTTTTTTTTTTTTTTTTT78141.3265404863076373No Hit
CATGGAAGCAGTGGTATCAACGCAG77071.3083756754508524No Hit
GTACATGGGAAGCAGTGGTATCAAC76191.2934363917555525No Hit
GTATCAACGCAGAGTACATGGAAGC67761.150324844538079No Hit
TATCAACGCAGAGTACTTTTTTTTT57070.9688465005576766No Hit
ACTCTGCGTTGATACCACTGCTTCC48420.8220001324163781No Hit
CAGTGGTATCAACGCAGAGTACATG48170.8177560177302136No Hit
ACGCAGAGTACATGGAAGCAGTGGT47580.8077399070708648No Hit
GAGTACTTTTTTTTTTTTTTTTTTT44400.7537547682628499No Hit
TATCAACGCAGAGTACATGGAAGCA38810.6588563638802073No Hit
GTATCAACGCAGAGTACATGGGAAG37430.6354288508125783No Hit
ACGCAGAGTACTTTTTTTTTTTTTT34350.5831413578790291No Hit
GTACATGGGGTGGTATCAACGCAAA32010.5434164444165276No Hit
GGTATCAACGCAGAGTACTTTTTTT28420.4824709575232026No Hit
ATACCACTGCTTCCATGTACTCTGC27260.46277826537939837No Hit
GTACTCTGCGTTGATACCACTGCTT26650.45242262554515655No Hit
GGTATCAACGCAGAGTACATGGAAG25770.4374833418498568No Hit
GCGTTGATACCACTGCTTCCCATGT25670.43578569597539096No Hit
AAGCAGTGGTATCAACGCAGAGTAC25380.43086252293943983No Hit
AAAAAGTACTCTGCGTTGATACCAC23780.4037001889479858No Hit
GAGTACATGGGAAGCAGTGGTATCA23410.3974188992124621No Hit
GTGGTATCAACGCAGAGTACATGGA22950.38960972818991907No Hit
GAAGCAGTGGTATCAACGCAGAGTA22060.37450067990717273No Hit
CCACTGCTTCCATGTACTCTGCGTT21570.36618221512228993No Hit
TATCAACGCAGAGTACATGGGAAGC20870.35429869400102876No Hit
GCTTCCCATGTACTCTGCGTTGATA19360.32866424129659405No Hit
GTACATGGGTGGTATCAACGCAAAA19240.3266270662472349No Hit
ACATGGAAGCAGTGGTATCAACGCA18860.32017601192426465No Hit
TGGAAGCAGTGGTATCAACGCAGAG17670.2999740260181207No Hit
CTGCTTCCATGTACTCTGCGTTGAT17090.2901276799462186No Hit
ACGCAGAGTACATGGGAAGCAGTGG17060.28961838618387886No Hit
GCAGAGTACTTTTTTTTTTTTTTTT16350.2775651004751711No Hit
GATACCACTGCTTCCATGTACTCTG16260.2760372191881518No Hit
GTATCAACGCAGAGTACATGGGGTG15850.2690768711028417No Hit
GGTATCAACGCAGAGTACATGGGAA15580.2644932272417838No Hit
GCAGAGTACATGGAAGCAGTGGTAT15340.2604188771430657No Hit
CCCCATGTACTCTGCGTTGATACCA15300.2597398187932794No Hit
GTTGATACCACTGCTTCCATGTACT14750.25040276648371707No Hit
CCATGTACTCTGCGTTGATACCACT14560.24717723932223185No Hit
GTACATGGGAGTGGTATCAACGCAA14110.23953783288713543No Hit
GCAGTGGTATCAACGCAGAGTACAT12920.21933584698099148No Hit
ATCAACGCAGAGTACATGGAAGCAG12820.21763820110652557No Hit
CTTCCATGTACTCTGCGTTGATACC12560.2132243218329143No Hit
ATCAACGCAGAGTACTTTTTTTTTT11420.19387115886400327No Hit
TATCAACGCAGAGTACATGGGGTGG11380.19319210051421692No Hit
CACTGCTTCCATGTACTCTGCGTTG9790.16619953111020946No Hit
CTGCTTCCCATGTACTCTGCGTTGA9080.15414624540150174No Hit
TCCATGTACTCTGCGTTGATACCAA9040.1534671870517154No Hit
AAAGTACTCTGCGTTGATACCACTG8960.1521090703521427No Hit
GTGGTATCAACGCAGAGTACATGGG8830.14990213071533703No Hit
TGATACCACTGCTTCCATGTACTCT8360.14192319510534743No Hit
GTATCAACGCAGAGTACATGGGAGT8260.14022554923088154No Hit
GTATCAACGCAGAGTACATGGAATC8180.13886743253130884No Hit
GTATCAACGCAGAGTACATGGGCAG7790.1322466136208919No Hit
GGTATCAACGCAGAGTACATGGGGT7520.12766296975983404No Hit
GTACATGGAAGCAGTGGTATCAACC7500.12732344058494086No Hit
CTGCGTTGATACCACTGCTTCCATG7010.11900497580005806No Hit
ACGCAGAGTACATGGGGTGGTATCA6750.11459109652644678No Hit
GATACCACTGCTTCCCATGTACTCT6450.10949815890304913No Hit
CTCTGCGTTGATACCACTGCTTCCA6360.10797027761602984No Hit
GTATCAACGCAGAGTACATGGGTGG6220.10559357339177762No Hit
TCCATGTACTCTGCGTTGATACAAC6090.10338663375497198No Hit
GAGTACATGGGGTGGTATCAACGCA6010.10202851705539927No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGTTG307.71354E-419.000519
GGAGGGC250.00602678619.000516
CAGGGGG250.006029745618.9988964
AAAGTAA250.006032706718.9972811
GGTCGAT751.70985E-1017.73079511
CCTAGAA453.5449426E-516.8821682
GATCGGT855.638867E-1116.7651567
CAGGATC855.638867E-1116.7637314
CCAGGGG402.7622632E-416.6212063
AACCCGT350.002166424216.28753715
ATAGTTC350.00216765516.2861529
GTTGGTT350.00216765516.2861528
AGGCATA350.002168886116.284775
GTCCTAA350.002178757516.2737031
GGATCGG1001.8189894E-1216.1504336
TCAGGAT901.3460522E-1015.8297213
TCGATGG851.0131771E-915.64880913
TCCTCGG551.1256454E-515.54587310
GGATTTG508.6783824E-515.20428619
TCGGATG508.690981E-515.201713
Produced by FastQC (version 0.11.5)