##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062351_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	589051
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.320933161984275	32.0	32.0	32.0	21.0	32.0
2	30.454627867536086	32.0	32.0	32.0	32.0	32.0
3	30.51858667585659	32.0	32.0	32.0	32.0	32.0
4	30.319632765244435	32.0	32.0	32.0	21.0	32.0
5	30.482871601949576	32.0	32.0	32.0	32.0	32.0
6	33.86542082094759	36.0	36.0	36.0	32.0	36.0
7	33.53242758267111	36.0	36.0	36.0	21.0	36.0
8	33.458680148238436	36.0	36.0	36.0	21.0	36.0
9	33.40605312613	36.0	36.0	36.0	21.0	36.0
10	33.446103987600395	36.0	36.0	36.0	21.0	36.0
11	33.76114122546265	36.0	36.0	36.0	32.0	36.0
12	33.61204547653769	36.0	36.0	36.0	27.0	36.0
13	33.60103115010415	36.0	36.0	36.0	27.0	36.0
14	33.54226034757602	36.0	36.0	36.0	27.0	36.0
15	33.64928164114822	36.0	36.0	36.0	27.0	36.0
16	33.49587047641036	36.0	36.0	36.0	27.0	36.0
17	33.404715381180914	36.0	36.0	36.0	21.0	36.0
18	33.47079454919863	36.0	36.0	36.0	27.0	36.0
19	33.42711921378624	36.0	36.0	36.0	21.0	36.0
20	33.252519730889176	36.0	36.0	36.0	21.0	36.0
21	32.98894493006548	36.0	36.0	36.0	14.0	36.0
22	32.94458883865743	36.0	36.0	36.0	14.0	36.0
23	32.747522710257684	36.0	36.0	36.0	14.0	36.0
24	32.760647210513184	36.0	36.0	36.0	14.0	36.0
25	31.967119994703346	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	3.0
4	23.0
5	47.0
6	112.0
7	31.0
8	57.0
9	117.0
10	74.0
11	21.0
12	41.0
13	30.0
14	474.0
15	575.0
16	813.0
17	1257.0
18	1749.0
19	2385.0
20	3258.0
21	4171.0
22	5229.0
23	6216.0
24	6880.0
25	7969.0
26	9354.0
27	11026.0
28	13996.0
29	17857.0
30	23334.0
31	32200.0
32	45804.0
33	61782.0
34	120671.0
35	211494.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.10950801191175	15.234856485081444	14.575601275386335	20.080034227620466
2	10.252805889013954	23.811391289732764	37.31785701552895	28.617945805724336
3	20.850084207095126	33.391508665181725	24.813250393871897	20.945156733851253
4	12.529542799706608	19.44408600690011	30.19460894841216	37.83176224498112
5	11.370448551501145	40.05087874521738	32.34383570716531	16.234836996116165
6	31.375333103479903	29.06513627307946	21.900163218582122	17.65936740485851
7	25.3890895254144	33.17614603470125	23.601027379807775	17.833737060076576
8	32.59280907047356	27.20938174861141	23.08311825563634	17.114690925278694
9	28.141407962481523	18.82703777335984	17.36606004995667	35.66549421420197
10	16.96625546857207	29.01580999452718	31.0230776290787	22.994856907822054
11	32.823271466449114	24.659392208851724	16.67057583792236	25.846760486776805
12	21.90593722802437	26.163715731070496	29.311663674934724	22.61868336597041
13	34.8793177001273	22.436149112027778	23.498418493345095	19.18611469449982
14	29.045033101326602	17.579481426713915	24.416286362593375	28.9591991093661
15	29.098998660802295	20.377048734560137	33.71175298940198	16.812199615235585
16	25.99602660036403	25.370955434363278	28.603548867887014	20.02946909738568
17	23.212218764288057	23.287005325143074	32.27159079513412	21.229185115434756
18	27.521847255345588	19.798432403121595	34.51415885729633	18.165561484236488
19	20.31289838961357	36.051646592079635	26.785890335981616	16.849564682325184
20	21.65059368615707	19.14361664156857	40.141206544314855	19.06458312795951
21	22.30715621281659	28.978242393336735	23.062043175250725	25.652558218595956
22	19.811046486916574	29.506432478106454	25.84773209880531	24.83478893617166
23	18.87893870779106	28.607393959174793	24.680585587423334	27.833081745610816
24	17.69868332035805	33.93280276322544	25.644996243400453	22.723517673016055
25	26.585426383997174	24.29882236153229	22.475080571685	26.64067068278554
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	693.0
1	693.0
2	1414.5
3	2136.0
4	2136.0
5	2136.0
6	5716.5
7	9297.0
8	9297.0
9	9297.0
10	7873.0
11	6449.0
12	6449.0
13	6449.0
14	4232.0
15	2015.0
16	2015.0
17	2015.0
18	3123.0
19	4231.0
20	4231.0
21	4231.0
22	5923.5
23	7616.0
24	7616.0
25	7616.0
26	10622.0
27	13628.0
28	13628.0
29	13628.0
30	15593.0
31	17558.0
32	17558.0
33	17558.0
34	17627.5
35	17697.0
36	17697.0
37	17697.0
38	21700.0
39	25703.0
40	25703.0
41	25703.0
42	45849.0
43	65995.0
44	65995.0
45	65995.0
46	139203.0
47	212411.0
48	212411.0
49	212411.0
50	154905.5
51	97400.0
52	97400.0
53	97400.0
54	67302.0
55	37204.0
56	37204.0
57	37204.0
58	31017.5
59	24831.0
60	24831.0
61	24831.0
62	21523.0
63	18215.0
64	18215.0
65	18215.0
66	15187.5
67	12160.0
68	12160.0
69	12160.0
70	9611.5
71	7063.0
72	7063.0
73	7063.0
74	5527.0
75	3991.0
76	3991.0
77	3991.0
78	2839.0
79	1687.0
80	1687.0
81	1687.0
82	1116.5
83	546.0
84	546.0
85	546.0
86	352.0
87	158.0
88	158.0
89	158.0
90	101.5
91	45.0
92	45.0
93	45.0
94	39.5
95	34.0
96	34.0
97	34.0
98	161.0
99	288.0
100	288.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008997523134669155
2	0.003734820923824932
3	0.004583643861057871
4	0.012732344058494086
5	0.03395291748931756
6	0.04583643861057871
7	0.0626431327677909
8	0.08556135207308026
9	0.091842641808604
10	0.116967800750699
11	0.11730732992559219
12	0.1295303802217465
13	0.11679803616325242
14	0.11985379873729099
15	0.10847957137836961
16	0.10830980679092303
17	0.12053285708707735
18	0.12936061563429993
19	0.12647461764770793
20	0.11764685910048535
21	0.12749320517238744
22	0.14667660355385187
23	0.13360473032046463
24	0.12783273434728062
25	0.12817226352217378
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	589051.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.880090173426073
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.13117550012237	15.125963537376208
2	14.50352929685106	6.346770576960561
3	6.039448660907062	3.9643104389527157
4	3.05881312628383	2.6770842810699804
5	1.8743408966846355	2.050537391760005
6	1.1125291230407843	1.4605342519616995
7	0.813971656776807	1.2466841274222689
8	0.5221303381436895	0.9139407104692301
9	0.35918956995053536	0.7073190161884662
>10	2.129494225839903	8.642797102731649
>50	0.22109474878843258	3.3779838974119993
>100	0.17066963064370233	7.276049331514132
>500	0.02249736040303349	3.3776444186021495
>1k	0.031030841935218607	15.844155013317202
>5k	0.006981939435424187	11.773294865001441
>10k+	0.0031030841935218603	15.214931039260307
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	35333	5.998292168250288	No Hit
TCCATGTACTCTGCGTTGATACCAC	29923	5.079865750164247	No Hit
GAGTACATGGAAGCAGTGGTATCAA	13051	2.215597630765418	No Hit
CATGTACTCTGCGTTGATACCACTG	11330	1.92343277576984	No Hit
CCCATGTACTCTGCGTTGATACCAC	8515	1.4455454621076953	No Hit
GCGTTGATACCACTGCTTCCATGTA	8469	1.437736291085152	No Hit
GCTTCCATGTACTCTGCGTTGATAC	8459	1.4360386452106864	No Hit
GTATCAACGCAGAGTACTTTTTTTT	8295	1.408197252869446	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	7814	1.3265404863076373	No Hit
CATGGAAGCAGTGGTATCAACGCAG	7707	1.3083756754508524	No Hit
GTACATGGGAAGCAGTGGTATCAAC	7619	1.2934363917555525	No Hit
GTATCAACGCAGAGTACATGGAAGC	6776	1.150324844538079	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5707	0.9688465005576766	No Hit
ACTCTGCGTTGATACCACTGCTTCC	4842	0.8220001324163781	No Hit
CAGTGGTATCAACGCAGAGTACATG	4817	0.8177560177302136	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	4758	0.8077399070708648	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	4440	0.7537547682628499	No Hit
TATCAACGCAGAGTACATGGAAGCA	3881	0.6588563638802073	No Hit
GTATCAACGCAGAGTACATGGGAAG	3743	0.6354288508125783	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3435	0.5831413578790291	No Hit
GTACATGGGGTGGTATCAACGCAAA	3201	0.5434164444165276	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2842	0.4824709575232026	No Hit
ATACCACTGCTTCCATGTACTCTGC	2726	0.46277826537939837	No Hit
GTACTCTGCGTTGATACCACTGCTT	2665	0.45242262554515655	No Hit
GGTATCAACGCAGAGTACATGGAAG	2577	0.4374833418498568	No Hit
GCGTTGATACCACTGCTTCCCATGT	2567	0.43578569597539096	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	2538	0.43086252293943983	No Hit
AAAAAGTACTCTGCGTTGATACCAC	2378	0.4037001889479858	No Hit
GAGTACATGGGAAGCAGTGGTATCA	2341	0.3974188992124621	No Hit
GTGGTATCAACGCAGAGTACATGGA	2295	0.38960972818991907	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	2206	0.37450067990717273	No Hit
CCACTGCTTCCATGTACTCTGCGTT	2157	0.36618221512228993	No Hit
TATCAACGCAGAGTACATGGGAAGC	2087	0.35429869400102876	No Hit
GCTTCCCATGTACTCTGCGTTGATA	1936	0.32866424129659405	No Hit
GTACATGGGTGGTATCAACGCAAAA	1924	0.3266270662472349	No Hit
ACATGGAAGCAGTGGTATCAACGCA	1886	0.32017601192426465	No Hit
TGGAAGCAGTGGTATCAACGCAGAG	1767	0.2999740260181207	No Hit
CTGCTTCCATGTACTCTGCGTTGAT	1709	0.2901276799462186	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	1706	0.28961838618387886	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	1635	0.2775651004751711	No Hit
GATACCACTGCTTCCATGTACTCTG	1626	0.2760372191881518	No Hit
GTATCAACGCAGAGTACATGGGGTG	1585	0.2690768711028417	No Hit
GGTATCAACGCAGAGTACATGGGAA	1558	0.2644932272417838	No Hit
GCAGAGTACATGGAAGCAGTGGTAT	1534	0.2604188771430657	No Hit
CCCCATGTACTCTGCGTTGATACCA	1530	0.2597398187932794	No Hit
GTTGATACCACTGCTTCCATGTACT	1475	0.25040276648371707	No Hit
CCATGTACTCTGCGTTGATACCACT	1456	0.24717723932223185	No Hit
GTACATGGGAGTGGTATCAACGCAA	1411	0.23953783288713543	No Hit
GCAGTGGTATCAACGCAGAGTACAT	1292	0.21933584698099148	No Hit
ATCAACGCAGAGTACATGGAAGCAG	1282	0.21763820110652557	No Hit
CTTCCATGTACTCTGCGTTGATACC	1256	0.2132243218329143	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	1142	0.19387115886400327	No Hit
TATCAACGCAGAGTACATGGGGTGG	1138	0.19319210051421692	No Hit
CACTGCTTCCATGTACTCTGCGTTG	979	0.16619953111020946	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	908	0.15414624540150174	No Hit
TCCATGTACTCTGCGTTGATACCAA	904	0.1534671870517154	No Hit
AAAGTACTCTGCGTTGATACCACTG	896	0.1521090703521427	No Hit
GTGGTATCAACGCAGAGTACATGGG	883	0.14990213071533703	No Hit
TGATACCACTGCTTCCATGTACTCT	836	0.14192319510534743	No Hit
GTATCAACGCAGAGTACATGGGAGT	826	0.14022554923088154	No Hit
GTATCAACGCAGAGTACATGGAATC	818	0.13886743253130884	No Hit
GTATCAACGCAGAGTACATGGGCAG	779	0.1322466136208919	No Hit
GGTATCAACGCAGAGTACATGGGGT	752	0.12766296975983404	No Hit
GTACATGGAAGCAGTGGTATCAACC	750	0.12732344058494086	No Hit
CTGCGTTGATACCACTGCTTCCATG	701	0.11900497580005806	No Hit
ACGCAGAGTACATGGGGTGGTATCA	675	0.11459109652644678	No Hit
GATACCACTGCTTCCCATGTACTCT	645	0.10949815890304913	No Hit
CTCTGCGTTGATACCACTGCTTCCA	636	0.10797027761602984	No Hit
GTATCAACGCAGAGTACATGGGTGG	622	0.10559357339177762	No Hit
TCCATGTACTCTGCGTTGATACAAC	609	0.10338663375497198	No Hit
GAGTACATGGGGTGGTATCAACGCA	601	0.10202851705539927	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	1.697645874465878E-4
10	0.0	0.0	0.0	5.092937623397634E-4	3.395291748931756E-4
11	0.0	0.0	0.0	5.092937623397634E-4	3.395291748931756E-4
12	0.0	0.0	0.0	5.092937623397634E-4	5.092937623397634E-4
13	0.0	0.0	0.0	8.488229372329391E-4	6.790583497863512E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGGTTG	30	7.71354E-4	19.00051	9
GGAGGGC	25	0.006026786	19.00051	6
CAGGGGG	25	0.0060297456	18.998896	4
AAAGTAA	25	0.0060327067	18.99728	11
GGTCGAT	75	1.70985E-10	17.730795	11
CCTAGAA	45	3.5449426E-5	16.882168	2
GATCGGT	85	5.638867E-11	16.765156	7
CAGGATC	85	5.638867E-11	16.763731	4
CCAGGGG	40	2.7622632E-4	16.621206	3
AACCCGT	35	0.0021664242	16.287537	15
ATAGTTC	35	0.002167655	16.286152	9
GTTGGTT	35	0.002167655	16.286152	8
AGGCATA	35	0.0021688861	16.28477	5
GTCCTAA	35	0.0021787575	16.273703	1
GGATCGG	100	1.8189894E-12	16.150433	6
TCAGGAT	90	1.3460522E-10	15.829721	3
TCGATGG	85	1.0131771E-9	15.648809	13
TCCTCGG	55	1.1256454E-5	15.545873	10
GGATTTG	50	8.6783824E-5	15.204286	19
TCGGATG	50	8.690981E-5	15.2017	13
>>END_MODULE