##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062351_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	589051
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.56732099597488	32.0	32.0	32.0	32.0	32.0
2	31.354473551526098	32.0	32.0	32.0	32.0	32.0
3	31.471504165174153	32.0	32.0	32.0	32.0	32.0
4	31.634681886627813	32.0	32.0	32.0	32.0	32.0
5	31.5495415507316	32.0	32.0	32.0	32.0	32.0
6	35.1240351005261	36.0	36.0	36.0	36.0	36.0
7	35.12762901684234	36.0	36.0	36.0	36.0	36.0
8	35.13195631617636	36.0	36.0	36.0	36.0	36.0
9	35.093241502009164	36.0	36.0	36.0	36.0	36.0
10	35.07685582402882	36.0	36.0	36.0	36.0	36.0
11	35.118986301695436	36.0	36.0	36.0	36.0	36.0
12	35.02792118169734	36.0	36.0	36.0	36.0	36.0
13	35.0139886020056	36.0	36.0	36.0	36.0	36.0
14	34.94567363437122	36.0	36.0	36.0	32.0	36.0
15	34.96587562027736	36.0	36.0	36.0	36.0	36.0
16	34.89459656294616	36.0	36.0	36.0	32.0	36.0
17	34.87312304028004	36.0	36.0	36.0	32.0	36.0
18	34.844443010876816	36.0	36.0	36.0	32.0	36.0
19	34.71893774902343	36.0	36.0	36.0	32.0	36.0
20	34.64212436614147	36.0	36.0	36.0	32.0	36.0
21	34.415924936890015	36.0	36.0	36.0	32.0	36.0
22	34.35968532436071	36.0	36.0	36.0	32.0	36.0
23	34.15246897127753	36.0	36.0	36.0	32.0	36.0
24	34.106124936550486	36.0	36.0	36.0	32.0	36.0
25	34.05800516423875	36.0	36.0	36.0	32.0	36.0
26	33.90465851004412	36.0	36.0	36.0	32.0	36.0
27	33.77272426326413	36.0	36.0	36.0	32.0	36.0
28	33.577642682891636	36.0	36.0	36.0	27.0	36.0
29	33.46551996346666	36.0	36.0	36.0	21.0	36.0
30	33.3883874231603	36.0	36.0	36.0	21.0	36.0
31	33.24566463684808	36.0	36.0	36.0	14.0	36.0
32	33.11522092314588	36.0	36.0	36.0	14.0	36.0
33	32.914861361749665	36.0	36.0	36.0	14.0	36.0
34	32.728994603183764	36.0	36.0	36.0	14.0	36.0
35	32.369343231740544	36.0	36.0	36.0	14.0	36.0
36	32.29245854773186	36.0	36.0	36.0	14.0	36.0
37	31.96321710683795	36.0	36.0	36.0	14.0	36.0
38	31.727366560790152	36.0	32.0	36.0	14.0	36.0
39	31.003201760119243	36.0	32.0	36.0	14.0	36.0
40	30.518832834508387	36.0	32.0	36.0	14.0	36.0
41	30.648347935917265	36.0	32.0	36.0	14.0	36.0
42	29.853033099001614	36.0	14.0	36.0	14.0	36.0
43	30.10011696780075	36.0	21.0	36.0	14.0	36.0
44	30.10010168898788	36.0	21.0	36.0	14.0	36.0
45	29.193658953129695	36.0	14.0	36.0	14.0	36.0
46	29.808952026225235	36.0	14.0	36.0	14.0	36.0
47	29.292937283868458	36.0	14.0	36.0	14.0	36.0
48	29.452763852365923	36.0	14.0	36.0	14.0	36.0
49	29.6471952343685	36.0	14.0	36.0	14.0	36.0
50	28.768014993608364	36.0	14.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	9.0
20	12.0
21	51.0
22	192.0
23	594.0
24	1774.0
25	4767.0
26	10287.0
27	19308.0
28	30080.0
29	37581.0
30	44361.0
31	52936.0
32	70430.0
33	55760.0
34	74080.0
35	186829.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.29510629375141	16.083710679971	15.191152176385994	20.430030849891594
2	10.39056067229744	24.035142820763124	36.373838122320784	29.20045838461865
3	20.78336570194619	32.561692765275495	25.53102633401692	21.12391519876139
4	12.68837503034542	19.25469950819199	29.81320802443252	38.24371743703007
5	11.424307912218127	39.48299892539016	32.59259385010805	16.500099312283655
6	31.86955901726175	29.091347394439843	21.10507123358368	17.934022354714727
7	24.90208827419018	32.7020920090111	23.919830371224222	18.475989345574494
8	32.641316286705226	27.05504277218781	23.212760864509185	17.09088007659778
9	27.991803765718075	17.651612508933862	17.78250100585518	36.57408271949288
10	17.139942042369846	28.602786515938348	31.3938860981477	22.863385343544106
11	32.96692476542778	23.947162469803125	16.928924660173735	26.156988104595357
12	21.552463199281558	26.266316498910957	29.868890809115	22.312329492692484
13	35.49862405801875	21.530563567500945	24.088406606558685	18.88240576792162
14	28.914983592252625	17.016183658121285	24.854893718880028	29.213939030746065
15	29.552789147289456	20.103692210012376	33.46891865050734	16.874599992190827
16	25.949196249560735	24.40009438911062	29.20171598044991	20.448993380878736
17	22.99648078010223	22.741664134344905	33.09085291426379	21.171002171289075
18	27.92304910780221	18.320824512648308	35.234640124539304	18.521486255010178
19	20.817552300225277	34.66949381292961	27.656179176336178	16.856774710508937
20	21.78504068408338	18.245109506647132	40.9884712868665	18.981378522402984
21	22.369540158661984	28.085513817988595	23.97619221425649	25.568753809092932
22	19.928325391180053	27.924407224501785	26.86982960728358	25.277437777034585
23	19.120245954934294	26.414351219164384	25.996900098633226	28.468502727268095
24	17.947002891090925	32.571543041264675	26.57868333981268	22.902770727831715
25	26.962351307441974	22.168029593362885	23.458749751719292	27.410869347475852
26	18.467840645377056	22.311820198930143	29.396435962251143	29.82390319344165
27	26.938754029786892	27.503391047634246	28.737749362958386	16.820105559620472
28	23.800995160011613	19.206486365357158	29.246703596123258	27.745814878507975
29	18.827062512414035	28.399917833939675	34.78697090744265	17.98604874620364
30	28.00470587436402	21.868564861107103	31.482503212794814	18.644226051734062
31	17.355882597601905	25.431244493261197	30.070910668176438	27.14196224096046
32	18.65118639981937	27.953946262717487	30.153586022262928	23.24128131520021
33	18.804823351458534	26.493801046089388	29.320720956249968	25.38065464620211
34	16.61231370458585	29.77721793189384	33.418838097210596	20.19163026630971
35	24.447628473595664	26.196543253470413	29.417486771094524	19.9383415018394
36	22.395344375953865	28.656262360984027	29.5478659742535	19.400527288808608
37	24.93145754781844	28.108262272706437	29.867872221590318	17.0924079578848
38	22.124230329801662	28.41808264479646	24.171421489820066	25.28626553558181
39	19.123641246683224	28.03254726670526	31.600319836482747	21.243491650128764
40	23.116164814252077	28.140347779733844	24.564935803521255	24.178551602492824
41	17.31055545275367	28.293984731373005	29.678414941999932	24.7170448738734
42	26.483105877080256	27.613907794061976	27.93798839149751	17.964997937360263
43	24.74132120987826	23.12397398527462	24.150710210151583	27.983994594695538
44	22.864913224831128	25.147058573875608	33.70166589989662	18.286362301396654
45	25.690984312054475	25.82085422145111	27.191193971319972	21.29696749517444
46	20.762548573892584	25.580297801039297	32.81922957434925	20.83792405071887
47	24.656311009252185	23.829216535098887	32.38349885408709	19.130973601561838
48	19.6049238520943	37.1127457554609	23.005138774062008	20.27719161838279
49	18.54729047230206	26.08127309859418	36.31654984033641	19.054886588767356
50	18.394332579012683	36.29838502947962	20.791238789171057	24.51604360233664
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	162.0
1	134.0
2	106.0
3	378.5
4	651.0
5	702.5
6	754.0
7	689.0
8	624.0
9	808.5
10	993.0
11	1498.5
12	2004.0
13	3499.0
14	4994.0
15	6766.5
16	8539.0
17	9600.0
18	10661.0
19	11361.0
20	12061.0
21	12145.0
22	12229.0
23	11891.5
24	11554.0
25	8587.0
26	5620.0
27	4656.5
28	3693.0
29	3860.0
30	4027.0
31	5389.0
32	6751.0
33	7668.0
34	8585.0
35	8275.0
36	7965.0
37	8109.5
38	8254.0
39	10037.0
40	11820.0
41	16545.5
42	21271.0
43	30349.5
44	39428.0
45	57297.0
46	75166.0
47	88472.5
48	101779.0
49	88808.0
50	75837.0
51	57269.5
52	38702.0
53	30616.0
54	22530.0
55	20830.0
56	19130.0
57	17879.5
58	16629.0
59	15474.5
60	14320.0
61	13030.5
62	11741.0
63	10289.5
64	8838.0
65	7959.0
66	7080.0
67	5888.5
68	4697.0
69	4159.5
70	3622.0
71	3070.0
72	2518.0
73	2091.0
74	1664.0
75	1341.5
76	1019.0
77	762.5
78	506.0
79	382.0
80	258.0
81	201.0
82	144.0
83	106.0
84	68.0
85	50.0
86	32.0
87	19.5
88	7.0
89	7.5
90	8.0
91	4.5
92	1.0
93	1.0
94	1.0
95	3.0
96	5.0
97	4.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.011883521121261146
2	0.004413879273611284
3	5.092937623397634E-4
4	0.0
5	0.0
6	5.092937623397634E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	1.697645874465878E-4
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	589051.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.02260240733561
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.59711687856515	28.743991182145628
2	11.540986372092837	8.776366724291291
3	4.751670344303452	5.42012616816533
4	2.32102562056577	3.5300573739204673
5	1.408740168611516	2.6781983663179307
6	0.8722407277726558	1.9898917437350852
7	0.5897346882815415	1.569627330484014
8	0.4341975416117203	1.3207456392756003
9	0.32562876066964813	1.1143127609401928
>10	1.8293981085217081	13.339085430652094
>50	0.17836836175989737	4.654424503011216
>100	0.12296606757499581	9.049727532866365
>500	0.015314455302380433	3.9945467530246423
>1k	0.011711054054761508	9.463671088948535
>5k	0.0	0.0
>10k+	9.008503119047313E-4	4.355227402221602
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	13754	2.334942135740369	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	11676	1.9821713230263593	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	4553	0.7729381666443144	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	4252	0.7218390258228914	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	3630	0.6162454524311137	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	3000	0.5092937623397634	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2963	0.5030124726042396	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	2926	0.4967311828687159	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	2887	0.49011036395829904	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	2774	0.4709269655768346	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	2742	0.46549449877854376	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2320	0.39385384287608377	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATA	2043	0.3468290521533789	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1966	0.3337571789199916	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	1673	0.2840161547981414	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC	1561	0.2650025210041236	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	1560	0.264832756416677	No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA	1525	0.2588909958560464	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1519	0.2578724083313669	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	1486	0.25227017694562953	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTATA	1362	0.23121936810225258	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	1258	0.21356385100780748	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	1255	0.2130545572454677	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1226	0.20813138420951666	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	1223	0.2076220904471769	No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT	1222	0.20745232585973034	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1173	0.19913386107484754	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTCTCTTATA	1159	0.19675715685059528	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	959	0.16280423936127772	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	951	0.16144612266170502	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	917	0.15567412668852101	No Hit
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA	904	0.1534671870517154	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	898	0.15244859952703588	No Hit
GATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	886	0.1504114244776768	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	859	0.14582778061661894	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	836	0.14192319510534743	No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTG	811	0.13767908041918273	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTATGCGTTGATA	773	0.13122802609621237	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	749	0.12715367599749428	No Hit
ACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAA	725	0.12307932589877615	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	716	0.12155144461175686	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	709	0.12036309249963076	No Hit
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	693	0.11764685910048535	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA	692	0.11747709451303877	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	677	0.11493062570133997	No Hit
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	644	0.10932839431560255	No Hit
TGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC	630	0.10695169009135033	No Hit
GTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT	614	0.10423545669220491	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	597	0.10134945870561292	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	590	0.10016110659348681	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	3.395291748931756E-4
3	0.0	0.0	0.0	0.0	3.395291748931756E-4
4	0.0	0.0	0.0	0.0	3.395291748931756E-4
5	0.0	0.0	0.0	0.0	3.395291748931756E-4
6	0.0	0.0	0.0	0.0	3.395291748931756E-4
7	0.0	0.0	0.0	0.0	5.092937623397634E-4
8	0.0	0.0	0.0	0.0	5.092937623397634E-4
9	0.0	0.0	0.0	0.0	0.0010185875246795269
10	0.0	0.0	0.0	5.092937623397634E-4	0.0010185875246795269
11	0.0	0.0	0.0	5.092937623397634E-4	0.0010185875246795269
12	0.0	0.0	0.0	5.092937623397634E-4	0.0011883521121261148
13	0.0	0.0	0.0	0.0010185875246795269	0.0011883521121261148
14	0.0	0.0	0.0	0.0010185875246795269	0.0011883521121261148
15	0.0	0.0	0.0	0.0011883521121261148	0.0011883521121261148
16	0.0	0.0	0.0	0.001867410461912466	0.0011883521121261148
17	0.0	0.0	0.0	0.002546468811698817	0.0013581166995727024
18	0.0	0.0	0.0	0.002716233399145405	0.0023767042242522295
19	0.0	0.0	0.0	0.004413879273611283	0.0023767042242522295
20	0.0	0.0	0.0	0.00543246679829081	0.0023767042242522295
21	0.0	0.0	0.0	0.008488229372329391	0.002716233399145405
22	0.0	0.0	0.0	0.016806694157212195	0.002716233399145405
23	0.0	0.0	0.0	0.03361338831442439	0.002716233399145405
24	0.0	0.0	0.0	0.05330608045822857	0.002716233399145405
25	0.0	0.0	0.0	0.06909418709076125	0.002716233399145405
26	0.0	0.0	0.0	0.08233582491159509	0.002716233399145405
27	0.0	0.0	0.0	0.10151922329305951	0.002716233399145405
28	0.0	0.0	0.0	0.13071873233387263	0.0030557625740385806
29	0.0	0.0	0.0	0.1797806981059365	0.0030557625740385806
30	0.0	0.0	0.0	0.25294923529541585	0.0030557625740385806
31	0.0	0.0	0.0	0.3729727986201534	0.0030557625740385806
32	0.0	0.0	0.0	0.5171029333623065	0.0030557625740385806
33	0.0	0.0	0.0	0.627280150615142	0.0033952917489317564
34	0.0	0.0	0.0	0.7790496917923915	0.0033952917489317564
35	0.0	0.0	0.0	0.9391376977545238	0.0033952917489317564
36	0.0	0.0	0.0	1.1572851926233891	0.0033952917489317564
37	0.0	0.0	0.0	1.486458727682323	0.003734820923824932
38	0.0	0.0	0.0	1.7667400615566393	0.003734820923824932
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTGTG	20	7.8536524E-4	43.99966	5
ATACATG	235	0.0	38.39246	1
CTTGCTA	30	1.300384E-4	36.666386	42
TTAGGGT	30	0.00574287	29.333109	4
GCGACTA	30	0.00574287	29.333109	16
TAGCACC	30	0.00574287	29.333109	4
TATAGTA	30	0.00574287	29.333109	37
TCAGTAC	30	0.00574287	29.333109	3
CATGCGA	45	4.0662577E-5	29.333107	41
ACAATGC	80	4.307367E-9	27.499786	37
GCGATTA	40	7.028142E-4	27.499786	42
TATAGCC	90	5.12955E-10	26.888681	3
TGTCATA	50	8.334741E-5	26.399797	44
ACTCACT	75	7.000199E-8	26.399797	38
AAAAGTA	625	0.0	25.345955	2
TAGCCTG	105	1.1277734E-10	25.142662	5
CGTAGAA	45	0.0013967354	24.446333	2
ATAGCCT	100	1.7407729E-9	24.199814	4
TCGTATA	200	0.0	24.199814	44
TGGTATA	900	0.0	24.199814	44
>>END_MODULE