FastQCFastQC Report
Thu 2 Feb 2017
SRR4062350_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062350_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340994
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG42471.2454764599963637No Hit
TCCATGTACTCTGCGTTGATACCAC38911.1410757960550626No Hit
GTATCAACGCAGAGTACTTTTTTTT22630.6636480407279893No Hit
GAGTACATGGAAGCAGTGGTATCAA16860.4944368522613301No Hit
TATCAACGCAGAGTACTTTTTTTTT16700.489744687589811No Hit
CATGTACTCTGCGTTGATACCACTG14360.4211217792688435No Hit
GTACTTTTTTTTTTTTTTTTTTTTT11210.32874478729831025No Hit
GCTTCCATGTACTCTGCGTTGATAC10770.3158413344516326No Hit
GCGTTGATACCACTGCTTCCATGTA10260.3008850595611653No Hit
CATGGAAGCAGTGGTATCAACGCAG9980.2926737713860068No Hit
GAGTACTTTTTTTTTTTTTTTTTTT9150.2683331671525012No Hit
ACGCAGAGTACTTTTTTTTTTTTTT7700.22581042481685892No Hit
GGTATCAACGCAGAGTACTTTTTTT7670.22493064394094908No Hit
CCCATGTACTCTGCGTTGATACCAC7660.22463738364897917No Hit
GTATCAACGCAGAGTACATGGAAGC7010.20557546467093263No Hit
GTACATGGGGTGGTATCAACGCAAA6910.20264286175123314No Hit
ACTCTGCGTTGATACCACTGCTTCC5570.1633459826272603No Hit
ACGCAGAGTACATGGAAGCAGTGGT5360.15718751649589144No Hit
CAGTGGTATCAACGCAGAGTACATG5100.1495627489046728No Hit
GTACATGGGAAGCAGTGGTATCAAC4850.14223124160542414No Hit
TATCAACGCAGAGTACATGGAAGCA4800.1407649401455744No Hit
GTATCAACGCAGAGTACATGGGCAG4650.1363660357660252No Hit
GTATCAACGCAGAGTACATGGGAAG4560.1337266931382957No Hit
GTACATGGGTGGTATCAACGCAAAA4330.12698170642298692No Hit
ATTCCATTCCATTCCATTCCATTCC4270.12522214467116724No Hit
GCAGAGTACTTTTTTTTTTTTTTTT3670.10762652715297044No Hit
ATACCACTGCTTCCATGTACTCTGC3630.10645348598509063No Hit
GAATGGAATGGAATGGAATGGAATG3490.1023478418975114No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACCCT250.006025315718.9985314
CTACTCA453.5281533E-516.8875854
TTAAGAC350.00216876916.2820683
GCCTACA350.00216876916.2820682
GATCTAT350.002173025616.277291
ACGTTAG551.1286822E-515.5374141
ACTTCCA508.644455E-515.20775318
TTTCGAC456.747126E-414.77663713
ATTTCGA456.747126E-414.77663712
TCAATAG456.7546405E-414.7744693
GTGTACC400.005270616614.2488994
TAAGACA602.5585334E-514.2488994
AATAGAC400.005275752414.2468083
TCTGGAG400.005275752414.2468082
GATTAAG400.005286036514.2426291
GACGAAC500.001492923613.30287716
TCGACTT500.001494576713.30092415
ACCGTAA500.001496231413.2989728
TAAGCTG500.001496231413.2989725
CCTACTC500.001497887713.2970213
Produced by FastQC (version 0.11.5)