##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062350_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	340994
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.46398177094025	32.0	32.0	32.0	14.0	32.0
2	27.974181363894967	32.0	27.0	32.0	14.0	32.0
3	28.323615664791756	32.0	32.0	32.0	14.0	32.0
4	27.79048604960791	32.0	21.0	32.0	14.0	32.0
5	28.03239646445392	32.0	21.0	32.0	14.0	32.0
6	31.161765309653543	36.0	32.0	36.0	14.0	36.0
7	31.000542531540145	36.0	32.0	36.0	14.0	36.0
8	30.856314187346406	36.0	32.0	36.0	14.0	36.0
9	31.170099767151328	36.0	32.0	36.0	14.0	36.0
10	30.00594731872115	36.0	21.0	36.0	14.0	36.0
11	31.512355056100695	36.0	32.0	36.0	14.0	36.0
12	30.597934274503363	36.0	32.0	36.0	14.0	36.0
13	31.062825152348722	36.0	32.0	36.0	14.0	36.0
14	30.759632134289753	36.0	32.0	36.0	14.0	36.0
15	30.72179569141979	36.0	32.0	36.0	14.0	36.0
16	30.665835762506084	36.0	32.0	36.0	14.0	36.0
17	30.49131949535769	36.0	27.0	36.0	14.0	36.0
18	30.60459714833692	36.0	32.0	36.0	14.0	36.0
19	30.386115298216392	36.0	27.0	36.0	14.0	36.0
20	30.53063983530502	36.0	27.0	36.0	14.0	36.0
21	30.283221992175815	36.0	27.0	36.0	14.0	36.0
22	30.14549816125797	36.0	27.0	36.0	14.0	36.0
23	30.06195710188449	36.0	27.0	36.0	14.0	36.0
24	30.058537686880122	36.0	27.0	36.0	14.0	36.0
25	29.25479040686933	32.0	21.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	20.0
5	38.0
6	55.0
7	21.0
8	55.0
9	48.0
10	25.0
11	24.0
12	19.0
13	17.0
14	1917.0
15	2590.0
16	2385.0
17	2494.0
18	2631.0
19	3098.0
20	3924.0
21	4776.0
22	6169.0
23	7677.0
24	9232.0
25	11589.0
26	14043.0
27	16508.0
28	19383.0
29	22827.0
30	26102.0
31	30879.0
32	36005.0
33	39789.0
34	47896.0
35	28756.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.396236541794856	18.177045603188617	12.10219290656171	23.324524948454815
2	15.6445667060628	21.3208360583141	37.67687935691432	25.357717878708787
3	19.344119984632393	27.29157685122457	27.82533719283119	25.53896597131185
4	12.807449772693943	15.961577943980057	35.42190937087549	35.80906291245051
5	14.512854812780887	37.00613698501543	32.099482521913615	16.381525680290068
6	31.50296309335211	35.704981517338496	17.888282579358094	14.903772809951299
7	28.143405063253997	31.31740269801537	21.503129686092336	19.036062552638295
8	29.1633770159811	31.154777453296745	20.277357909102918	19.404487621619243
9	27.736227868313527	14.297245573662707	18.85713614802987	39.10939040999389
10	17.28974980770247	26.464837442530932	29.698371783053158	26.547040966713443
11	36.931324423984776	23.08317871151091	19.63736289545999	20.34813396904432
12	22.95052604070319	24.685594148512905	29.781861212169257	22.58201859861465
13	30.571197059462506	21.679417999048788	25.048000986442293	22.701383955046417
14	24.40135055783911	18.17586611861421	25.53934233705226	31.88344098649442
15	27.364001996300974	24.757948507177876	24.647858380060477	23.230191116460674
16	25.962927298988923	26.78640628486208	23.719424122503142	23.531242293645853
17	22.649375662431996	25.59563829818468	27.098276282218787	24.656709757164542
18	24.15055670393686	25.419876914403833	28.132634125716432	22.296932255942874
19	24.468912867943917	26.620861777875653	25.959039859061882	22.95118549511855
20	25.242356450410142	22.710164585131558	28.431597057079273	23.615881907379027
21	26.568422783420097	24.38949912062035	24.040977036299736	25.00110105965982
22	23.899624104311055	25.539175378832375	26.283918712557263	24.277281804299307
23	23.580952716531158	26.112642888393626	25.306330987218224	25.000073407856988
24	23.007267121779343	26.603831755120016	25.278426191000513	25.110474932100125
25	25.19781907225424	24.78647284557047	24.313762335248644	25.701945746926647
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	114.0
1	114.0
2	294.5
3	475.0
4	475.0
5	475.0
6	1014.5
7	1554.0
8	1554.0
9	1554.0
10	1624.0
11	1694.0
12	1694.0
13	1694.0
14	1485.5
15	1277.0
16	1277.0
17	1277.0
18	2128.5
19	2980.0
20	2980.0
21	2980.0
22	4233.0
23	5486.0
24	5486.0
25	5486.0
26	8075.5
27	10665.0
28	10665.0
29	10665.0
30	12974.0
31	15283.0
32	15283.0
33	15283.0
34	17486.5
35	19690.0
36	19690.0
37	19690.0
38	23341.0
39	26992.0
40	26992.0
41	26992.0
42	31547.5
43	36103.0
44	36103.0
45	36103.0
46	46975.5
47	57848.0
48	57848.0
49	57848.0
50	51213.5
51	44579.0
52	44579.0
53	44579.0
54	39204.0
55	33829.0
56	33829.0
57	33829.0
58	30043.5
59	26258.0
60	26258.0
61	26258.0
62	23466.0
63	20674.0
64	20674.0
65	20674.0
66	18095.0
67	15516.0
68	15516.0
69	15516.0
70	12367.0
71	9218.0
72	9218.0
73	9218.0
74	7628.5
75	6039.0
76	6039.0
77	6039.0
78	4343.0
79	2647.0
80	2647.0
81	2647.0
82	1986.5
83	1326.0
84	1326.0
85	1326.0
86	898.0
87	470.0
88	470.0
89	470.0
90	285.0
91	100.0
92	100.0
93	100.0
94	62.5
95	25.0
96	25.0
97	25.0
98	88.5
99	152.0
100	152.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009091069051068348
2	0.003812383795609307
3	0.004985424963489094
4	0.012903452846677654
5	0.032258632116694136
6	0.03929687912397286
7	0.065983565693238
8	0.08181962145961513
9	0.08973764934280369
10	0.11026586978069995
11	0.11085239036463986
12	0.12639518583904702
13	0.11026586978069995
14	0.115544555036159
15	0.1067467462770606
16	0.10733326686100048
17	0.11525129474418905
18	0.1225828020434377
19	0.12287606233540767
20	0.11143891094857973
21	0.12111650058358799
22	0.13900537839375474
23	0.12639518583904702
24	0.12287606233540767
25	0.11877041824782841
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	340994.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.98759578635753
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.11888507190476	34.35680616107793
2	14.771173256483335	13.881238365317868
3	5.122590476348397	7.2209463204510955
4	2.2311109641282414	4.19338160547873
5	1.1717363558599212	2.7528537128662776
6	0.7499416528560402	2.1142773148651486
7	0.46953600452751	1.5443657591515985
8	0.3339430493660901	1.2552944815421985
9	0.26672999078784954	1.1279700892114508
>10	1.5459324845613462	13.611858720888243
>50	0.1404133027563393	4.556800079605491
>100	0.06615026707631985	5.869299253113481
>500	0.00624059123361508	2.114484258303423
>1k	0.0056165321102535715	5.4004238781270475
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	4247	1.2454764599963637	No Hit
TCCATGTACTCTGCGTTGATACCAC	3891	1.1410757960550626	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2263	0.6636480407279893	No Hit
GAGTACATGGAAGCAGTGGTATCAA	1686	0.4944368522613301	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1670	0.489744687589811	No Hit
CATGTACTCTGCGTTGATACCACTG	1436	0.4211217792688435	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1121	0.32874478729831025	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1077	0.3158413344516326	No Hit
GCGTTGATACCACTGCTTCCATGTA	1026	0.3008850595611653	No Hit
CATGGAAGCAGTGGTATCAACGCAG	998	0.2926737713860068	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	915	0.2683331671525012	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	770	0.22581042481685892	No Hit
GGTATCAACGCAGAGTACTTTTTTT	767	0.22493064394094908	No Hit
CCCATGTACTCTGCGTTGATACCAC	766	0.22463738364897917	No Hit
GTATCAACGCAGAGTACATGGAAGC	701	0.20557546467093263	No Hit
GTACATGGGGTGGTATCAACGCAAA	691	0.20264286175123314	No Hit
ACTCTGCGTTGATACCACTGCTTCC	557	0.1633459826272603	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	536	0.15718751649589144	No Hit
CAGTGGTATCAACGCAGAGTACATG	510	0.1495627489046728	No Hit
GTACATGGGAAGCAGTGGTATCAAC	485	0.14223124160542414	No Hit
TATCAACGCAGAGTACATGGAAGCA	480	0.1407649401455744	No Hit
GTATCAACGCAGAGTACATGGGCAG	465	0.1363660357660252	No Hit
GTATCAACGCAGAGTACATGGGAAG	456	0.1337266931382957	No Hit
GTACATGGGTGGTATCAACGCAAAA	433	0.12698170642298692	No Hit
ATTCCATTCCATTCCATTCCATTCC	427	0.12522214467116724	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	367	0.10762652715297044	No Hit
ATACCACTGCTTCCATGTACTCTGC	363	0.10645348598509063	No Hit
GAATGGAATGGAATGGAATGGAATG	349	0.1023478418975114	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	5.865205839398934E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACCCT	25	0.0060253157	18.998531	4
CTACTCA	45	3.5281533E-5	16.887585	4
TTAAGAC	35	0.002168769	16.282068	3
GCCTACA	35	0.002168769	16.282068	2
GATCTAT	35	0.0021730256	16.27729	1
ACGTTAG	55	1.1286822E-5	15.537414	1
ACTTCCA	50	8.644455E-5	15.207753	18
TTTCGAC	45	6.747126E-4	14.776637	13
ATTTCGA	45	6.747126E-4	14.776637	12
TCAATAG	45	6.7546405E-4	14.774469	3
GTGTACC	40	0.0052706166	14.248899	4
TAAGACA	60	2.5585334E-5	14.248899	4
AATAGAC	40	0.0052757524	14.246808	3
TCTGGAG	40	0.0052757524	14.246808	2
GATTAAG	40	0.0052860365	14.242629	1
GACGAAC	50	0.0014929236	13.302877	16
TCGACTT	50	0.0014945767	13.300924	15
ACCGTAA	50	0.0014962314	13.298972	8
TAAGCTG	50	0.0014962314	13.298972	5
CCTACTC	50	0.0014978877	13.297021	3
>>END_MODULE