FastQCFastQC Report
Thu 2 Feb 2017
SRR4062350_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062350_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340994
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA22930.6724458494870877No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC16360.47977383766283277No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC8380.24575212467081534No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA7320.21466653372200095No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA6910.20264286175123314No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC4890.14340428277330391No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG4800.1407649401455744No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG4790.14047167985360445No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA4530.13284691226238585No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA3940.115544555036159No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3810.1117321712405497No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3700.10850630802888028No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAG254.436235E-544.032
ACTATAC353.2130565E-431.428573
TAGACTA353.2130565E-431.428575
GTCTATA353.2130565E-431.428571
GACGGTT454.0610812E-529.3333347
CTAGACG602.934903E-729.3333324
TGGACCT300.005739304229.3333325
GTAGAAC300.005739304229.3333323
TCGTGCG407.021711E-427.532
ACTAGAC756.981463E-826.43
CGACCTA701.1282846E-625.14285530
ACGGAGC450.001396158524.44444526
TCAAACG450.001396158524.44444522
GGGGCTA1500.023.46666736
CGCTTAA1700.023.29411714
GCTAGTT1800.023.22222339
GGGCTAG1850.022.59459537
GGCTAGT1850.022.59459538
CGGAAGG500.002576675722.015
TCCGAGA602.866924E-422.02
Produced by FastQC (version 0.11.5)