##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062350_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	340994
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.736097995859165	32.0	32.0	32.0	32.0	32.0
2	30.64314328111345	32.0	32.0	32.0	32.0	32.0
3	31.075359097227516	32.0	32.0	32.0	32.0	32.0
4	31.36614720493616	32.0	32.0	32.0	32.0	32.0
5	31.1444160307806	32.0	32.0	32.0	32.0	32.0
6	34.71419145204901	36.0	36.0	36.0	32.0	36.0
7	34.76223335307952	36.0	36.0	36.0	32.0	36.0
8	34.65217569810613	36.0	36.0	36.0	32.0	36.0
9	34.850592679050074	36.0	36.0	36.0	32.0	36.0
10	34.66697654504185	36.0	36.0	36.0	32.0	36.0
11	34.87776911030693	36.0	36.0	36.0	32.0	36.0
12	34.75507486935254	36.0	36.0	36.0	32.0	36.0
13	34.80455081321079	36.0	36.0	36.0	32.0	36.0
14	34.74496325448542	36.0	36.0	36.0	32.0	36.0
15	34.72061091984023	36.0	36.0	36.0	32.0	36.0
16	34.69733191786366	36.0	36.0	36.0	32.0	36.0
17	34.68301201780677	36.0	36.0	36.0	32.0	36.0
18	34.70185985677168	36.0	36.0	36.0	32.0	36.0
19	34.663794670873976	36.0	36.0	36.0	32.0	36.0
20	34.63177064699086	36.0	36.0	36.0	32.0	36.0
21	34.594019249605566	36.0	36.0	36.0	32.0	36.0
22	34.5652181563312	36.0	36.0	36.0	32.0	36.0
23	34.50505874003648	36.0	36.0	36.0	32.0	36.0
24	34.474219487732924	36.0	36.0	36.0	32.0	36.0
25	34.44798735461621	36.0	36.0	36.0	32.0	36.0
26	34.3723760535376	36.0	36.0	36.0	32.0	36.0
27	34.36722640281061	36.0	36.0	36.0	32.0	36.0
28	34.34104705654645	36.0	36.0	36.0	32.0	36.0
29	34.274066405860516	36.0	36.0	36.0	32.0	36.0
30	34.263004627647405	36.0	36.0	36.0	32.0	36.0
31	34.19568966022862	36.0	36.0	36.0	32.0	36.0
32	34.13847457726529	36.0	36.0	36.0	32.0	36.0
33	34.06115943389033	36.0	36.0	36.0	32.0	36.0
34	34.014695273230615	36.0	36.0	36.0	32.0	36.0
35	33.923893089027956	36.0	36.0	36.0	32.0	36.0
36	33.85414992639166	36.0	36.0	36.0	32.0	36.0
37	33.81960972920345	36.0	36.0	36.0	32.0	36.0
38	33.71555804500959	36.0	36.0	36.0	32.0	36.0
39	33.57984891229758	36.0	36.0	36.0	27.0	36.0
40	33.488929423978135	36.0	36.0	36.0	21.0	36.0
41	33.429858003366625	36.0	36.0	36.0	21.0	36.0
42	33.32076224215089	36.0	36.0	36.0	21.0	36.0
43	33.30769163093779	36.0	36.0	36.0	21.0	36.0
44	33.25086658416277	36.0	36.0	36.0	21.0	36.0
45	33.0330181762729	36.0	36.0	36.0	14.0	36.0
46	33.10762359455005	36.0	36.0	36.0	14.0	36.0
47	32.935532590016244	36.0	36.0	36.0	14.0	36.0
48	32.88181903493903	36.0	36.0	36.0	14.0	36.0
49	32.88424429755362	36.0	36.0	36.0	14.0	36.0
50	32.150222584561604	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	3.0
21	15.0
22	39.0
23	139.0
24	373.0
25	882.0
26	1947.0
27	3771.0
28	6516.0
29	9712.0
30	14105.0
31	20110.0
32	29782.0
33	44725.0
34	78950.0
35	129925.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.10377837151396	18.699463584452456	12.518806805350666	23.677951238682923
2	15.973523606748724	21.806952252751604	36.54962270846347	25.669901432036202
3	19.631896443014625	26.80539955600001	28.456762788460694	25.10594121252467
4	13.216948098793527	16.18034334914984	35.77687583945758	34.825832712599045
5	15.278274691050283	36.17013789098928	31.74982551012628	16.801761907834155
6	32.35276867041649	35.829076171428234	17.129040393672614	14.68911476448266
7	27.92483152196226	31.105239388376337	21.797450981542195	19.172478108119204
8	28.835111468236978	31.535745496988216	20.319125849721694	19.31001718505311
9	27.481128700211734	13.778541557915974	18.895347132207604	39.84498260966468
10	17.074787239658175	26.12567963072664	30.168859276116294	26.630673853498887
11	37.44083473609506	22.5127128336569	19.7950697079714	20.251382722276638
12	22.853188032634005	24.457615090001582	29.901699150131673	22.787497727232736
13	31.09116289436178	21.254332920813855	25.065836935547253	22.588667249277115
14	24.463773556132953	17.948996170020585	25.611007818319383	31.976222455527076
15	27.548578567364824	24.52975712182619	24.54383361584075	23.377830694968242
16	26.18110582590896	26.541815985031995	23.846460641536215	23.430617547522832
17	22.76257060241529	25.221264890291323	27.48552760459128	24.5306369027021
18	24.552338164307876	24.8467714974457	28.183193839187787	22.417696499058636
19	24.74119779233652	26.11864138371936	26.389027372915653	22.751133451028462
20	25.044428934233448	22.60567634621137	28.87147574444125	23.478418975113932
21	26.57759374065233	24.268168941389	24.505416517592685	24.64882080036599
22	23.92417461890825	25.169944339196583	26.736247558608067	24.169633483287097
23	23.694845070587753	25.437104465181203	25.80104048751591	25.06700997671513
24	23.069614128107826	26.289318873645872	25.595465022844976	25.045601975401325
25	25.592239159633305	23.942943277594328	24.615682387373386	25.84913517539898
26	23.3860419831434	24.21919447263002	26.22392182853657	26.170841715690013
27	25.726552373355542	25.41774928591119	26.860883182695293	21.994815158037973
28	24.225352938761386	23.86083039584274	26.589617412623095	25.32419925277278
29	23.37049918766899	25.10982597934274	27.525704264591166	23.993970568397096
30	24.93386980416078	24.336205329126027	26.989917711162075	23.740007155551123
31	23.247036604749642	25.434758382845445	25.904561370581302	25.413643641823608
32	22.939113298181198	25.858519504742016	27.04944955043197	24.15291764664481
33	23.024745303436426	25.62391127116606	26.72099802342563	24.63034540197188
34	22.976650615553353	25.02947265934298	28.132459808676984	23.86141691642668
35	25.13797896737186	24.607764359490197	25.938579564449814	24.31567710868813
36	24.85439626503692	25.982861868537277	25.635934943136828	23.526806923288973
37	25.011877041824782	25.544730992334173	26.18403842882866	23.25935353701238
38	23.831797626937718	25.485492413356248	25.725672592479633	24.957037367226402
39	23.879599054528818	24.299547792629784	27.55356399232831	24.26728916051309
40	24.887827938321497	25.331530760072024	25.089004498612876	24.691636802993603
41	23.99133122576937	25.834765421092452	26.308087532331946	23.86581582080623
42	25.004838794817502	25.490477838319737	26.380229564156554	23.124453802706206
43	24.601605893358826	24.785480096423985	25.332410540947937	25.280503469269256
44	24.086934080951572	23.75613647160947	28.0084107051737	24.14851874226526
45	25.32361273218884	24.51392106605981	25.540332087954624	24.622134113796722
46	24.994574684598554	25.22449075350299	26.311020135251646	23.469914426646802
47	24.179897593506045	24.573452905329713	27.18669536707391	24.059954134090336
48	23.030610509275824	28.34038135568368	24.890760541241193	23.738247593799304
49	22.901869241101018	24.269928503140818	28.115157451450756	24.71304480430741
50	23.123574021830294	27.288749948679445	23.949981524601604	25.637694504888646
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	19.0
1	33.5
2	48.0
3	111.5
4	175.0
5	192.5
6	210.0
7	209.0
8	208.0
9	254.5
10	301.0
11	423.0
12	545.0
13	865.0
14	1185.0
15	1616.0
16	2047.0
17	2450.5
18	2854.0
19	3215.0
20	3576.0
21	3770.0
22	3964.0
23	4080.0
24	4196.0
25	3848.5
26	3501.0
27	3610.0
28	3719.0
29	4118.0
30	4517.0
31	5232.5
32	5948.0
33	6685.0
34	7422.0
35	8197.0
36	8972.0
37	10137.0
38	11302.0
39	12551.0
40	13800.0
41	15041.0
42	16282.0
43	17331.0
44	18380.0
45	20732.0
46	23084.0
47	25435.5
48	27787.0
49	26917.5
50	26048.0
51	24834.0
52	23620.0
53	21455.0
54	19290.0
55	19248.5
56	19207.0
57	17952.0
58	16697.0
59	15696.5
60	14696.0
61	13622.0
62	12548.0
63	11421.0
64	10294.0
65	9381.0
66	8468.0
67	7762.0
68	7056.0
69	6486.0
70	5916.0
71	5352.5
72	4789.0
73	4114.0
74	3439.0
75	2775.0
76	2111.0
77	1648.5
78	1186.0
79	958.5
80	731.0
81	555.0
82	379.0
83	338.0
84	297.0
85	206.0
86	115.0
87	74.5
88	34.0
89	24.0
90	14.0
91	9.5
92	5.0
93	4.5
94	4.0
95	4.0
96	4.0
97	3.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007918027883188562
2	0.0032258632116694136
3	8.7978087590984E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	340994.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.577044722754344
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.15588819877927	44.424080254449485
2	12.995785340701907	14.96517827537818
3	4.309743575876253	7.4442689583549075
4	1.9472045113901524	4.484571249466394
5	1.006029394090653	2.8962099707981492
6	0.5615843278285819	1.940061957539052
7	0.3671131124512432	1.479610166574034
8	0.2657460310785718	1.2240696893044318
9	0.18513313700710238	0.9593477018209567
>10	1.0914397109705012	11.574009887797201
>50	0.07536726903312219	3.020770893464032
>100	0.03640187824048668	3.760539471497858
>500	0.0015381075312881694	0.6674448343713524
>1k	0.0010254050208587796	1.1598366891840084
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	2293	0.6724458494870877	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	1636	0.47977383766283277	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	838	0.24575212467081534	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	732	0.21466653372200095	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	691	0.20264286175123314	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	489	0.14340428277330391	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	480	0.1407649401455744	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	479	0.14047167985360445	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	453	0.13284691226238585	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	394	0.115544555036159	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	381	0.1117321712405497	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	370	0.10850630802888028	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	2.932602919699467E-4	0.0	0.0	0.0	0.0
9	2.932602919699467E-4	0.0	0.0	0.0	0.0
10	2.932602919699467E-4	0.0	0.0	0.0	0.0
11	2.932602919699467E-4	0.0	0.0	0.0	0.0
12	2.932602919699467E-4	0.0	0.0	0.0	0.0
13	2.932602919699467E-4	0.0	0.0	0.0	0.0
14	2.932602919699467E-4	0.0	0.0	0.0	0.0
15	2.932602919699467E-4	0.0	0.0	2.932602919699467E-4	0.0
16	2.932602919699467E-4	0.0	0.0	5.865205839398934E-4	0.0
17	2.932602919699467E-4	0.0	0.0	5.865205839398934E-4	0.0
18	2.932602919699467E-4	0.0	0.0	8.7978087590984E-4	0.0
19	2.932602919699467E-4	0.0	0.0	0.0011730411678797868	0.0
20	2.932602919699467E-4	0.0	0.0	0.0011730411678797868	0.0
21	2.932602919699467E-4	0.0	0.0	0.00175956175181968	0.0
22	2.932602919699467E-4	0.0	0.0	0.00263934262772952	2.932602919699467E-4
23	2.932602919699467E-4	0.0	0.0	0.005865205839398934	2.932602919699467E-4
24	2.932602919699467E-4	0.0	0.0	0.009384329343038294	5.865205839398934E-4
25	2.932602919699467E-4	0.0	0.0	0.012610192554707708	5.865205839398934E-4
26	2.932602919699467E-4	0.0	0.0	0.016422576350317013	5.865205839398934E-4
27	2.932602919699467E-4	0.0	0.0	0.023460823357595736	5.865205839398934E-4
28	2.932602919699467E-4	0.0	0.0	0.03372493357654387	5.865205839398934E-4
29	2.932602919699467E-4	0.0	0.0	0.05630597605822976	5.865205839398934E-4
30	2.932602919699467E-4	0.0	0.0	0.09442981401432284	5.865205839398934E-4
31	2.932602919699467E-4	0.0	0.0	0.16745162671483957	5.865205839398934E-4
32	2.932602919699467E-4	0.0	0.0	0.25982861868537277	5.865205839398934E-4
33	2.932602919699467E-4	0.0	0.0	0.33695607547346873	5.865205839398934E-4
34	2.932602919699467E-4	0.0	0.0	0.4363713144512807	5.865205839398934E-4
35	2.932602919699467E-4	0.0	0.0	0.5522091297794096	5.865205839398934E-4
36	2.932602919699467E-4	0.0	0.0	0.6976662345965031	5.865205839398934E-4
37	2.932602919699467E-4	0.0	0.0	0.9299283858367009	5.865205839398934E-4
38	2.932602919699467E-4	0.0	0.0	1.1973817721132922	5.865205839398934E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTTAG	25	4.436235E-5	44.0	32
ACTATAC	35	3.2130565E-4	31.42857	3
TAGACTA	35	3.2130565E-4	31.42857	5
GTCTATA	35	3.2130565E-4	31.42857	1
GACGGTT	45	4.0610812E-5	29.333334	7
CTAGACG	60	2.934903E-7	29.333332	4
TGGACCT	30	0.0057393042	29.333332	5
GTAGAAC	30	0.0057393042	29.333332	3
TCGTGCG	40	7.021711E-4	27.5	32
ACTAGAC	75	6.981463E-8	26.4	3
CGACCTA	70	1.1282846E-6	25.142855	30
ACGGAGC	45	0.0013961585	24.444445	26
TCAAACG	45	0.0013961585	24.444445	22
GGGGCTA	150	0.0	23.466667	36
CGCTTAA	170	0.0	23.294117	14
GCTAGTT	180	0.0	23.222223	39
GGGCTAG	185	0.0	22.594595	37
GGCTAGT	185	0.0	22.594595	38
CGGAAGG	50	0.0025766757	22.0	15
TCCGAGA	60	2.866924E-4	22.0	2
>>END_MODULE