FastQCFastQC Report
Thu 2 Feb 2017
SRR4062349_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062349_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158080
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG87955.563638663967612No Hit
TCCATGTACTCTGCGTTGATACCAC70754.475581983805668No Hit
GTACTTTTTTTTTTTTTTTTTTTTT39422.4936740890688256No Hit
GTATCAACGCAGAGTACTTTTTTTT38982.46584008097166No Hit
GAGTACATGGAAGCAGTGGTATCAA34782.2001518218623484No Hit
TATCAACGCAGAGTACTTTTTTTTT28131.7794787449392713No Hit
CATGTACTCTGCGTTGATACCACTG27631.7478491902834008No Hit
GAGTACTTTTTTTTTTTTTTTTTTT26241.6599190283400809No Hit
GCTTCCATGTACTCTGCGTTGATAC21251.344256072874494No Hit
GCGTTGATACCACTGCTTCCATGTA19681.2449392712550607No Hit
CATGGAAGCAGTGGTATCAACGCAG19131.2101467611336032No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18801.1892712550607287No Hit
CCCATGTACTCTGCGTTGATACCAC14900.9425607287449393No Hit
GTATCAACGCAGAGTACATGGAAGC14890.9419281376518219No Hit
GGTATCAACGCAGAGTACTTTTTTT13990.884994939271255No Hit
GTACATGGGGTGGTATCAACGCAAA12930.8179402834008097No Hit
ACGCAGAGTACATGGAAGCAGTGGT11300.7148279352226721No Hit
ACTCTGCGTTGATACCACTGCTTCC10650.6737095141700405No Hit
TATCAACGCAGAGTACATGGAAGCA10130.6408147773279352No Hit
GTACATGGGAAGCAGTGGTATCAAC9750.6167763157894737No Hit
CAGTGGTATCAACGCAGAGTACATG9610.6079200404858299No Hit
GCAGAGTACTTTTTTTTTTTTTTTT8920.5642712550607287No Hit
GTATCAACGCAGAGTACATGGGAAG8700.5503542510121457No Hit
GTACATGGGTGGTATCAACGCAAAA8540.5402327935222672No Hit
GGTATCAACGCAGAGTACATGGAAG6800.43016194331983804No Hit
AAGCAGTGGTATCAACGCAGAGTAC6780.42889676113360325No Hit
AAAAAGTACTCTGCGTTGATACCAC6490.41055161943319834No Hit
ATACCACTGCTTCCATGTACTCTGC6440.4073886639676113No Hit
GTACATGGGAGTGGTATCAACGCAA6080.38461538461538464No Hit
GTACTCTGCGTTGATACCACTGCTT5490.3472925101214575No Hit
CCACTGCTTCCATGTACTCTGCGTT5360.3390688259109312No Hit
GTATCAACGCAGAGTACATGGGGTG5240.3314777327935223No Hit
GTGGTATCAACGCAGAGTACATGGA5130.3245192307692308No Hit
ATCAACGCAGAGTACTTTTTTTTTT4940.3125No Hit
GAAGCAGTGGTATCAACGCAGAGTA4930.3118674089068826No Hit
TATCAACGCAGAGTACATGGGAAGC4800.30364372469635625No Hit
GTATCAACGCAGAGTACATGGGCAG4780.3023785425101214No Hit
GCGTTGATACCACTGCTTCCCATGT4540.28719635627530365No Hit
ACATGGAAGCAGTGGTATCAACGCA4440.2808704453441295No Hit
GGTATCAACGCAGAGTACATGGGAA4320.2732793522267206No Hit
GTTGATACCACTGCTTCCATGTACT4060.256831983805668No Hit
TGGAAGCAGTGGTATCAACGCAGAG4060.256831983805668No Hit
TATCAACGCAGAGTACATGGGGTGG4010.253669028340081No Hit
GATACCACTGCTTCCATGTACTCTG3870.24481275303643726No Hit
CTGCTTCCATGTACTCTGCGTTGAT3820.2416497975708502No Hit
GTACATGGGCAGTGGTATCAACGCA3760.23785425101214577No Hit
GCAGAGTACATGGAAGCAGTGGTAT3430.21697874493927125No Hit
GGTATCAACGCAGAGTACATGGGGT3320.21002024291497975No Hit
GAGTACATGGGAAGCAGTGGTATCA3200.20242914979757085No Hit
GGTATCAACGCAGAGTACATGGGCA3010.1904099190283401No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT3000.18977732793522267No Hit
TATCAACGCAGAGTACATGGGCAGT2970.18787955465587045No Hit
GAGTACATGGGGTGGTATCAACGCA2900.18345141700404857No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA2850.18028846153846154No Hit
GTATCAACGCAGAGTACATGGGAGT2820.1783906882591093No Hit
GTATCAACGCAGAGTACATGGGTGG2810.1777580971659919No Hit
ACGCAGAGTACATGGGAAGCAGTGG2800.1771255060728745No Hit
AAAGTACTCTGCGTTGATACCACTG2770.17522773279352227No Hit
CTTCCATGTACTCTGCGTTGATACC2730.17269736842105263No Hit
GTACTCTGCGTTGATACCACTGCCC2590.1638410931174089No Hit
CAACGCAGAGTACTTTTTTTTTTTT2580.1632085020242915No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT2560.16194331983805668No Hit
ATCAACGCAGAGTACATGGAAGCAG2540.16067813765182185No Hit
CCCCATGTACTCTGCGTTGATACCA2410.15245445344129555No Hit
ACGCAGAGTACATGGGGTGGTATCA2360.1492914979757085No Hit
GCTTCCCATGTACTCTGCGTTGATA2360.1492914979757085No Hit
CCATGTACTCTGCGTTGATACCACT2190.13853744939271256No Hit
GTATCAACGCAGAGTACATGGGGCA2190.13853744939271256No Hit
TCCATGTACTCTGCGTTGATACCAA2170.13727226720647773No Hit
GTACATGGAAGCAGTGGTATCAACC2130.1347419028340081No Hit
GTATCAACGCAGAGTACATGGGACG2090.13221153846153846No Hit
GTATCAACGCAGAGTACATGGGGTA2090.13221153846153846No Hit
GCAGTGGTATCAACGCAGAGTACAT2070.13094635627530365No Hit
TATCAACGCAGAGTACATGGGTGGT2050.12968117408906885No Hit
CACTGCTTCCATGTACTCTGCGTTG2040.12904858299595143No Hit
TATCAACGCAGAGTACATGGGAGTG1970.12462044534412954No Hit
TGATACCACTGCTTCCATGTACTCT1900.1201923076923077No Hit
GTATCAACGCAGAGTACATGGAATC1840.11639676113360325No Hit
GCGTTGATACCACTGCCCATGTACT1840.11639676113360325No Hit
GCCCATGTACTCTGCGTTGATACCA1780.11260121457489877No Hit
GTATCAACGCAGAGTACATGGGGGT1710.10817307692307693No Hit
GTACATGGGGTATCAACGCAAAAAA1690.10690789473684212No Hit
GTGGTATCAACGCAGAGTACATGGG1680.1062753036437247No Hit
TATCAACGCAGAGTACATGGGGCAG1640.10374493927125505No Hit
AACGCAGAGTACTTTTTTTTTTTTT1640.10374493927125505No Hit
TATCAACGCAGAGTACATGGGGTAT1600.10121457489878542No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATAGG307.730069E-418.981953
ATACCAA350.002154026616.29087819
AAAAGTA2100.015.8182932
AAAAAGT2100.015.3663421
ATCAACC550.00304025212.09474319
AAGTACT3000.011.3963874
AAAGTAC2950.011.2604793
AGTACTC3050.010.9016395
ATGGGTG2950.010.3050845
TGATACA750.002618190110.13654617
TGGGTGG2350.09.7052046
TCCATGT16900.09.3224961
ACATGGA20750.09.1936673
GAGTGGT1451.6573995E-69.1753229
GGGGTGG2950.09.0198087
CATGGGT3550.08.8281884
TGGAAGC21600.08.7990866
TACTCTG25500.08.7949457
ATGGAAG22000.08.6795455
GAAGCAG21750.08.6510188
Produced by FastQC (version 0.11.5)