##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062349_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	158080
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.60936867408907	32.0	32.0	32.0	14.0	32.0
2	30.075183451417004	32.0	32.0	32.0	21.0	32.0
3	30.248779099190283	32.0	32.0	32.0	21.0	32.0
4	29.779453441295548	32.0	32.0	32.0	14.0	32.0
5	30.234672317813764	32.0	32.0	32.0	21.0	32.0
6	33.462000253036436	36.0	36.0	36.0	21.0	36.0
7	32.85986842105263	36.0	36.0	36.0	14.0	36.0
8	32.702726467611335	36.0	36.0	36.0	14.0	36.0
9	32.51001391700405	36.0	36.0	36.0	14.0	36.0
10	32.812898532388665	36.0	36.0	36.0	14.0	36.0
11	33.22293142712551	36.0	36.0	36.0	21.0	36.0
12	33.09016320850203	36.0	36.0	36.0	21.0	36.0
13	33.07126138663968	36.0	36.0	36.0	21.0	36.0
14	33.03588689271255	36.0	36.0	36.0	14.0	36.0
15	33.29019483805668	36.0	36.0	36.0	21.0	36.0
16	33.10397267206478	36.0	36.0	36.0	21.0	36.0
17	33.02732793522267	36.0	36.0	36.0	14.0	36.0
18	33.13106022267206	36.0	36.0	36.0	21.0	36.0
19	32.98858173076923	36.0	36.0	36.0	14.0	36.0
20	32.7455971659919	36.0	36.0	36.0	14.0	36.0
21	32.45983679149798	36.0	36.0	36.0	14.0	36.0
22	32.513233805668015	36.0	36.0	36.0	14.0	36.0
23	32.48715207489879	36.0	32.0	36.0	14.0	36.0
24	32.596938259109315	36.0	32.0	36.0	14.0	36.0
25	31.74266826923077	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	14.0
5	17.0
6	29.0
7	8.0
8	23.0
9	35.0
10	20.0
11	8.0
12	11.0
13	11.0
14	215.0
15	259.0
16	327.0
17	462.0
18	642.0
19	775.0
20	1197.0
21	1576.0
22	2061.0
23	2528.0
24	2692.0
25	3063.0
26	3168.0
27	3604.0
28	4169.0
29	5211.0
30	6238.0
31	8567.0
32	11719.0
33	16032.0
34	31503.0
35	51893.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	53.19441017757618	15.99324362794405	14.74508613109133	16.06726006338843
2	10.005946506743593	25.94574761507123	38.86548242617475	25.182823452010428
3	20.716007363779568	37.46860587963637	24.892611547975278	16.92277520860879
4	11.419098898456827	18.851509955647227	30.667948953186	39.06144219270995
5	9.839447654366303	43.57505837979458	30.431535847408824	16.153958118430296
6	28.103164556962025	30.5753164556962	23.372784810126582	17.94873417721519
7	24.290516373672983	34.87690466996271	23.80497192451588	17.027607031848426
8	34.141584290083784	25.04480315110219	23.919499977835898	16.89411258097813
9	29.570552924191386	17.592006281502496	18.9641853043236	33.873255489982526
10	17.391579734103534	29.901634775558804	31.025265864796904	21.681519625540755
11	31.94727708035115	25.67043741528483	18.150264121305785	24.232021383058232
12	21.991561929860126	27.97803061017636	30.416328806000404	19.614078653963105
13	35.13282407935241	23.505491582107705	25.84843993463307	15.513244403906814
14	28.792869721655624	17.946054148560133	28.40772320127706	24.853352928507178
15	25.98206255304595	22.13361877858147	37.94986128881063	13.934457379561952
16	23.661709566274826	26.54983279286583	30.69074280502635	19.097714835832996
17	19.40796766816797	25.019162184932505	34.45329177831411	21.11957836858542
18	23.233730940510956	20.642210552321348	38.86457072994596	17.259487777221732
19	18.007373995894888	35.39834781947647	31.758229227377544	14.836048957251096
20	20.549949641791613	18.700314817981997	44.09922150643889	16.65051403378751
21	22.725833016596987	27.75623970606867	26.565311035094386	22.95261624223996
22	19.19865174803908	28.814450625340548	30.80261540605953	21.184282220560842
23	17.505780987677785	28.189679749120973	29.353479679432354	24.951059583768888
24	15.148117176017434	33.69000557498353	30.84739749632558	20.31447975267346
25	23.824064160959878	25.712530012099865	28.235130152737042	22.228275674203214
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	472.0
1	472.0
2	725.0
3	978.0
4	978.0
5	978.0
6	2815.5
7	4653.0
8	4653.0
9	4653.0
10	4117.5
11	3582.0
12	3582.0
13	3582.0
14	2253.5
15	925.0
16	925.0
17	925.0
18	1564.5
19	2204.0
20	2204.0
21	2204.0
22	3009.5
23	3815.0
24	3815.0
25	3815.0
26	5097.5
27	6380.0
28	6380.0
29	6380.0
30	7024.0
31	7668.0
32	7668.0
33	7668.0
34	6803.0
35	5938.0
36	5938.0
37	5938.0
38	6615.5
39	7293.0
40	7293.0
41	7293.0
42	12496.5
43	17700.0
44	17700.0
45	17700.0
46	34373.0
47	51046.0
48	51046.0
49	51046.0
50	37475.0
51	23904.0
52	23904.0
53	23904.0
54	15726.5
55	7549.0
56	7549.0
57	7549.0
58	6069.5
59	4590.0
60	4590.0
61	4590.0
62	4105.5
63	3621.0
64	3621.0
65	3621.0
66	3139.5
67	2658.0
68	2658.0
69	2658.0
70	2133.5
71	1609.0
72	1609.0
73	1609.0
74	1207.5
75	806.0
76	806.0
77	806.0
78	599.5
79	393.0
80	393.0
81	393.0
82	265.0
83	137.0
84	137.0
85	137.0
86	87.0
87	37.0
88	37.0
89	37.0
90	22.5
91	8.0
92	8.0
93	8.0
94	9.0
95	10.0
96	10.0
97	10.0
98	57.0
99	104.0
100	104.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0044281376518218625
2	0.0025303643724696357
3	0.00569331983805668
4	0.018345141700404858
5	0.03985323886639676
6	0.05060728744939271
7	0.0714827935222672
8	0.1056427125506073
9	0.09868421052631579
10	0.12588562753036436
11	0.12525303643724697
12	0.1417004048582996
13	0.1277834008097166
14	0.13790485829959515
15	0.12525303643724697
16	0.12145748987854252
17	0.13727226720647773
18	0.13853744939271256
19	0.14423076923076925
20	0.13347672064777327
21	0.13917004048582995
22	0.15561740890688258
23	0.1486589068825911
24	0.14676113360323886
25	0.14359817813765183
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	158080.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.854504048582996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.69669873288977	24.0915991902834
2	11.393397465779548	6.802884615384615
3	3.2716023223291097	2.9301619433198383
4	1.2607534856125777	1.5055668016194332
5	0.7500953511039539	1.1196862348178138
6	0.4089502902911387	0.7325404858299596
7	0.3051235326524558	0.6376518218623481
8	0.20977242869856336	0.5010121457489879
9	0.18010764080179684	0.48393218623481776
>10	1.1060728058651523	6.553011133603238
>50	0.173750900538204	3.57413967611336
>100	0.173750900538204	11.792763157894736
>500	0.02966478789676654	6.283527327935223
>1k	0.03602152816035937	22.952302631578945
>5k	0.00423782684239522	10.039220647773279
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	8795	5.563638663967612	No Hit
TCCATGTACTCTGCGTTGATACCAC	7075	4.475581983805668	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3942	2.4936740890688256	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3898	2.46584008097166	No Hit
GAGTACATGGAAGCAGTGGTATCAA	3478	2.2001518218623484	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2813	1.7794787449392713	No Hit
CATGTACTCTGCGTTGATACCACTG	2763	1.7478491902834008	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2624	1.6599190283400809	No Hit
GCTTCCATGTACTCTGCGTTGATAC	2125	1.344256072874494	No Hit
GCGTTGATACCACTGCTTCCATGTA	1968	1.2449392712550607	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1913	1.2101467611336032	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1880	1.1892712550607287	No Hit
CCCATGTACTCTGCGTTGATACCAC	1490	0.9425607287449393	No Hit
GTATCAACGCAGAGTACATGGAAGC	1489	0.9419281376518219	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1399	0.884994939271255	No Hit
GTACATGGGGTGGTATCAACGCAAA	1293	0.8179402834008097	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1130	0.7148279352226721	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1065	0.6737095141700405	No Hit
TATCAACGCAGAGTACATGGAAGCA	1013	0.6408147773279352	No Hit
GTACATGGGAAGCAGTGGTATCAAC	975	0.6167763157894737	No Hit
CAGTGGTATCAACGCAGAGTACATG	961	0.6079200404858299	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	892	0.5642712550607287	No Hit
GTATCAACGCAGAGTACATGGGAAG	870	0.5503542510121457	No Hit
GTACATGGGTGGTATCAACGCAAAA	854	0.5402327935222672	No Hit
GGTATCAACGCAGAGTACATGGAAG	680	0.43016194331983804	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	678	0.42889676113360325	No Hit
AAAAAGTACTCTGCGTTGATACCAC	649	0.41055161943319834	No Hit
ATACCACTGCTTCCATGTACTCTGC	644	0.4073886639676113	No Hit
GTACATGGGAGTGGTATCAACGCAA	608	0.38461538461538464	No Hit
GTACTCTGCGTTGATACCACTGCTT	549	0.3472925101214575	No Hit
CCACTGCTTCCATGTACTCTGCGTT	536	0.3390688259109312	No Hit
GTATCAACGCAGAGTACATGGGGTG	524	0.3314777327935223	No Hit
GTGGTATCAACGCAGAGTACATGGA	513	0.3245192307692308	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	494	0.3125	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	493	0.3118674089068826	No Hit
TATCAACGCAGAGTACATGGGAAGC	480	0.30364372469635625	No Hit
GTATCAACGCAGAGTACATGGGCAG	478	0.3023785425101214	No Hit
GCGTTGATACCACTGCTTCCCATGT	454	0.28719635627530365	No Hit
ACATGGAAGCAGTGGTATCAACGCA	444	0.2808704453441295	No Hit
GGTATCAACGCAGAGTACATGGGAA	432	0.2732793522267206	No Hit
GTTGATACCACTGCTTCCATGTACT	406	0.256831983805668	No Hit
TGGAAGCAGTGGTATCAACGCAGAG	406	0.256831983805668	No Hit
TATCAACGCAGAGTACATGGGGTGG	401	0.253669028340081	No Hit
GATACCACTGCTTCCATGTACTCTG	387	0.24481275303643726	No Hit
CTGCTTCCATGTACTCTGCGTTGAT	382	0.2416497975708502	No Hit
GTACATGGGCAGTGGTATCAACGCA	376	0.23785425101214577	No Hit
GCAGAGTACATGGAAGCAGTGGTAT	343	0.21697874493927125	No Hit
GGTATCAACGCAGAGTACATGGGGT	332	0.21002024291497975	No Hit
GAGTACATGGGAAGCAGTGGTATCA	320	0.20242914979757085	No Hit
GGTATCAACGCAGAGTACATGGGCA	301	0.1904099190283401	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT	300	0.18977732793522267	No Hit
TATCAACGCAGAGTACATGGGCAGT	297	0.18787955465587045	No Hit
GAGTACATGGGGTGGTATCAACGCA	290	0.18345141700404857	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	285	0.18028846153846154	No Hit
GTATCAACGCAGAGTACATGGGAGT	282	0.1783906882591093	No Hit
GTATCAACGCAGAGTACATGGGTGG	281	0.1777580971659919	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	280	0.1771255060728745	No Hit
AAAGTACTCTGCGTTGATACCACTG	277	0.17522773279352227	No Hit
CTTCCATGTACTCTGCGTTGATACC	273	0.17269736842105263	No Hit
GTACTCTGCGTTGATACCACTGCCC	259	0.1638410931174089	No Hit
CAACGCAGAGTACTTTTTTTTTTTT	258	0.1632085020242915	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT	256	0.16194331983805668	No Hit
ATCAACGCAGAGTACATGGAAGCAG	254	0.16067813765182185	No Hit
CCCCATGTACTCTGCGTTGATACCA	241	0.15245445344129555	No Hit
ACGCAGAGTACATGGGGTGGTATCA	236	0.1492914979757085	No Hit
GCTTCCCATGTACTCTGCGTTGATA	236	0.1492914979757085	No Hit
CCATGTACTCTGCGTTGATACCACT	219	0.13853744939271256	No Hit
GTATCAACGCAGAGTACATGGGGCA	219	0.13853744939271256	No Hit
TCCATGTACTCTGCGTTGATACCAA	217	0.13727226720647773	No Hit
GTACATGGAAGCAGTGGTATCAACC	213	0.1347419028340081	No Hit
GTATCAACGCAGAGTACATGGGACG	209	0.13221153846153846	No Hit
GTATCAACGCAGAGTACATGGGGTA	209	0.13221153846153846	No Hit
GCAGTGGTATCAACGCAGAGTACAT	207	0.13094635627530365	No Hit
TATCAACGCAGAGTACATGGGTGGT	205	0.12968117408906885	No Hit
CACTGCTTCCATGTACTCTGCGTTG	204	0.12904858299595143	No Hit
TATCAACGCAGAGTACATGGGAGTG	197	0.12462044534412954	No Hit
TGATACCACTGCTTCCATGTACTCT	190	0.1201923076923077	No Hit
GTATCAACGCAGAGTACATGGAATC	184	0.11639676113360325	No Hit
GCGTTGATACCACTGCCCATGTACT	184	0.11639676113360325	No Hit
GCCCATGTACTCTGCGTTGATACCA	178	0.11260121457489877	No Hit
GTATCAACGCAGAGTACATGGGGGT	171	0.10817307692307693	No Hit
GTACATGGGGTATCAACGCAAAAAA	169	0.10690789473684212	No Hit
GTGGTATCAACGCAGAGTACATGGG	168	0.1062753036437247	No Hit
TATCAACGCAGAGTACATGGGGCAG	164	0.10374493927125505	No Hit
AACGCAGAGTACTTTTTTTTTTTTT	164	0.10374493927125505	No Hit
TATCAACGCAGAGTACATGGGGTAT	160	0.10121457489878542	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAATAGG	30	7.730069E-4	18.98195	3
ATACCAA	35	0.0021540266	16.290878	19
AAAAGTA	210	0.0	15.818293	2
AAAAAGT	210	0.0	15.366342	1
ATCAACC	55	0.003040252	12.094743	19
AAGTACT	300	0.0	11.396387	4
AAAGTAC	295	0.0	11.260479	3
AGTACTC	305	0.0	10.901639	5
ATGGGTG	295	0.0	10.305084	5
TGATACA	75	0.0026181901	10.136546	17
TGGGTGG	235	0.0	9.705204	6
TCCATGT	1690	0.0	9.322496	1
ACATGGA	2075	0.0	9.193667	3
GAGTGGT	145	1.6573995E-6	9.175322	9
GGGGTGG	295	0.0	9.019808	7
CATGGGT	355	0.0	8.828188	4
TGGAAGC	2160	0.0	8.799086	6
TACTCTG	2550	0.0	8.794945	7
ATGGAAG	2200	0.0	8.679545	5
GAAGCAG	2175	0.0	8.651018	8
>>END_MODULE