FastQCFastQC Report
Thu 2 Feb 2017
SRR4062348_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062348_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416228
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG382299.184629578019738No Hit
TCCATGTACTCTGCGTTGATACCAC313077.52159873915258No Hit
GAGTACATGGAAGCAGTGGTATCAA143533.448350423325677No Hit
CATGTACTCTGCGTTGATACCACTG116472.7982259723036416No Hit
CCCATGTACTCTGCGTTGATACCAC99092.3806663655496507No Hit
GCTTCCATGTACTCTGCGTTGATAC92962.233391314375775No Hit
GTACATGGGAAGCAGTGGTATCAAC89212.1432964625157367No Hit
GCGTTGATACCACTGCTTCCATGTA86282.07290235159576No Hit
CATGGAAGCAGTGGTATCAACGCAG80661.9378802002748492No Hit
GTATCAACGCAGAGTACATGGAAGC64571.5513132225607118No Hit
CAGTGGTATCAACGCAGAGTACATG52841.2694965259425124No Hit
ACTCTGCGTTGATACCACTGCTTCC52591.2634902024851764No Hit
ACGCAGAGTACATGGAAGCAGTGGT50541.2142383501350222No Hit
GTACTTTTTTTTTTTTTTTTTTTTT47621.144084492153339No Hit
GTATCAACGCAGAGTACTTTTTTTT47031.1299095687940264No Hit
TATCAACGCAGAGTACATGGAAGCA40380.9701413648288918No Hit
TATCAACGCAGAGTACTTTTTTTTT35890.8622677955351394No Hit
GTATCAACGCAGAGTACATGGGAAG31510.7570370085626148No Hit
GAGTACATGGGAAGCAGTGGTATCA27840.6688641802089239No Hit
GGTATCAACGCAGAGTACATGGAAG27640.6640591214430552No Hit
GAGTACTTTTTTTTTTTTTTTTTTT27580.6626176038132947No Hit
ATACCACTGCTTCCATGTACTCTGC27310.6561307744793718No Hit
AAGCAGTGGTATCAACGCAGAGTAC26800.6438778746264067No Hit
GTACTCTGCGTTGATACCACTGCTT26720.6419558511200592No Hit
GCGTTGATACCACTGCTTCCCATGT25630.6157682808460747No Hit
GTGGTATCAACGCAGAGTACATGGA24340.5847756518062216No Hit
GAAGCAGTGGTATCAACGCAGAGTA23060.5540232757046619No Hit
GCTTCCCATGTACTCTGCGTTGATA22650.544172905234631No Hit
GTACATGGGGTGGTATCAACGCAAA22170.532640764196546No Hit
ACATGGAAGCAGTGGTATCAACGCA21280.5112582526884304No Hit
CCACTGCTTCCATGTACTCTGCGTT21140.5078947115523224No Hit
AAAAAGTACTCTGCGTTGATACCAC20650.496122317575944No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19890.47786309426564283No Hit
TATCAACGCAGAGTACATGGGAAGC19370.46536994147438426No Hit
TGGAAGCAGTGGTATCAACGCAGAG18560.44590945347261596No Hit
CTGCTTCCATGTACTCTGCGTTGAT18130.43557857712599823No Hit
ACGCAGAGTACATGGGAAGCAGTGG17590.42260491845815273No Hit
GTTGATACCACTGCTTCCATGTACT16330.3923330482331799No Hit
GGTATCAACGCAGAGTACTTTTTTT16040.38536571302267025No Hit
GCAGAGTACATGGAAGCAGTGGTAT15400.3699895249718904No Hit
CCCCATGTACTCTGCGTTGATACCA15170.36446370739114137No Hit
GATACCACTGCTTCCATGTACTCTG14870.35725611924233835No Hit
GGTATCAACGCAGAGTACATGGGAA14490.34812650758718783No Hit
GTACATGGGTGGTATCAACGCAAAA14040.33731512536398317No Hit
GCAGTGGTATCAACGCAGAGTACAT13290.31929615499197556No Hit
CTTCCATGTACTCTGCGTTGATACC13230.3178546373622149No Hit
CCATGTACTCTGCGTTGATACCACT13180.3166533726707478No Hit
ATCAACGCAGAGTACATGGAAGCAG11620.27917391429697186No Hit
GTACATGGGAGTGGTATCAACGCAA10950.2630769674313117No Hit
TCCATGTACTCTGCGTTGATACCAA10370.24914229701029242No Hit
CTGCTTCCCATGTACTCTGCGTTGA10360.24890204407199898No Hit
GCAGAGTACTTTTTTTTTTTTTTTT9450.2270390266872964No Hit
GTGGTATCAACGCAGAGTACATGGG9440.22679877374900295No Hit
AAAGTACTCTGCGTTGATACCACTG9360.2248767502426555No Hit
CACTGCTTCCATGTACTCTGCGTTG9040.21718865621726552No Hit
GTACATGGAAGCAGTGGTATCAACC8410.2020527211047791No Hit
GTATCAACGCAGAGTACATGGAATC8390.20157221522819224No Hit
TGATACCACTGCTTCCATGTACTCT8250.19820867409208412No Hit
GTATCAACGCAGAGTACATGGGGTG8100.1946048800176826No Hit
ATACCACTGCTTCCCATGTACTCTG6790.1631317451012426No Hit
TATCAACGCAGAGTACATGGGGTGG6610.15880719221196077No Hit
TCCATGTACTCTGCGTTGATACAAC6600.1585669392736673No Hit
GATACCACTGCTTCCCATGTACTCT6540.1571254216439067No Hit
GGGAAGCAGTGGTATCAACGCAGAG6420.15424238638438548No Hit
CTGCGTTGATACCACTGCTTCCATG6410.15400213344609204No Hit
CATGGGAAGCAGTGGTATCAACGCA6280.15087884524827738No Hit
CTCTGCGTTGATACCACTGCTTCCA6270.15063859230998394No Hit
GTACATGGAAGCAGTGGTATCAAAG5990.14391151003776775No Hit
ATCAACGCAGAGTACTTTTTTTTTT5750.13814543951872532No Hit
GTATCAACGCAGAGTACATGGGAGT5720.137424680703845No Hit
GCAGAGTACATGGGAAGCAGTGGTA5270.12661329848064043No Hit
TCCATGTACTCTGCGTTGATACAAA5110.12276925146794546No Hit
AGTGGTATCAACGCAGAGTACATGG5060.12156798677647826No Hit
ATCAACGCAGAGTACATGGGAAGCA5000.12012646914671765No Hit
TACCACTGCTTCCATGTACTCTGCG4930.11844469857866362No Hit
TACCACTGCTTCCCATGTACTCTGC4650.11171761630644744No Hit
GTACATGGTAAGCAGTGGTATCAAC4550.10931508692351306No Hit
CCACTGCTTCCCATGTACTCTGCGT4510.10835407517033933No Hit
GGAAGCAGTGGTATCAACGCAGAGT4460.10715281047887215No Hit
GTTGATACCACTGCTTCCCATGTAC4410.10595154578740498No Hit
GTACATGGAAAGCAGTGGTATCAAC4340.10426977521935094No Hit
GAGTACATGGGGTGGTATCAACGCA4190.1006659811449494No Hit
ACATGGGAAGCAGTGGTATCAACGC4180.10042572820665598No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGAG250.006044447418.98933
ATACAAA907.2759576E-1216.8895319
ATAACAA350.002166055416.28633319
ATACCAA1550.015.32316319
TGATAAC1203.6379788E-1214.252254517
AAAAGTA3200.014.2402632
GTACATA400.005302912614.236841
AAAAAGT3200.014.236841
GATAACA1301.4551915E-1113.15592918
GGAGTGG2600.010.9632748
AAGTACT4200.010.8523344
ACATGGC700.001494433810.8510283
TTGATAA1855.456968E-1210.78549116
GATACAA3000.010.76837218
ATGGGGG803.7568464E-410.6879065
GGGGTGG4900.010.6649537
AAAGTAC4200.010.6249673
ATCAACC1701.364242E-1010.61805119
AGTACTC4450.010.247585
GGTGTGG856.561766E-410.0604158
Produced by FastQC (version 0.11.5)