##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062348_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	416228
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.557307533371134	32.0	32.0	32.0	32.0	32.0
2	30.741192327282164	32.0	32.0	32.0	32.0	32.0
3	30.760960339044946	32.0	32.0	32.0	32.0	32.0
4	30.61725544653411	32.0	32.0	32.0	32.0	32.0
5	30.768194355016963	32.0	32.0	32.0	32.0	32.0
6	34.20037095053672	36.0	36.0	36.0	32.0	36.0
7	33.90721431523107	36.0	36.0	36.0	32.0	36.0
8	33.909225232324594	36.0	36.0	36.0	32.0	36.0
9	33.831231920966395	36.0	36.0	36.0	32.0	36.0
10	33.914164832735906	36.0	36.0	36.0	32.0	36.0
11	34.09610598037614	36.0	36.0	36.0	32.0	36.0
12	34.00706343638583	36.0	36.0	36.0	32.0	36.0
13	33.87687997924215	36.0	36.0	36.0	32.0	36.0
14	33.80088797485993	36.0	36.0	36.0	32.0	36.0
15	33.88995214161469	36.0	36.0	36.0	32.0	36.0
16	33.68624407776507	36.0	36.0	36.0	27.0	36.0
17	33.623441959695164	36.0	36.0	36.0	27.0	36.0
18	33.597499447418244	36.0	36.0	36.0	27.0	36.0
19	33.61555926078976	36.0	36.0	36.0	27.0	36.0
20	33.521363291273055	36.0	36.0	36.0	27.0	36.0
21	33.13439268862258	36.0	36.0	36.0	14.0	36.0
22	33.04049463274936	36.0	36.0	36.0	14.0	36.0
23	32.61744284382598	36.0	36.0	36.0	14.0	36.0
24	32.63275416358342	36.0	36.0	36.0	14.0	36.0
25	31.640617642253765	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	19.0
5	34.0
6	77.0
7	13.0
8	58.0
9	80.0
10	52.0
11	13.0
12	28.0
13	19.0
14	291.0
15	352.0
16	465.0
17	624.0
18	839.0
19	1056.0
20	1498.0
21	2067.0
22	2681.0
23	3347.0
24	4049.0
25	4959.0
26	6060.0
27	7283.0
28	9257.0
29	12624.0
30	16881.0
31	23407.0
32	33688.0
33	45170.0
34	84193.0
35	155040.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.90906469772728	13.716581330475714	16.058020600821248	19.316333370975762
2	7.562419512945201	25.047331193418803	35.85205758548446	31.538191708151537
3	21.61865404483314	34.734869416880905	23.88073328367891	19.76574325460705
4	12.781402054424222	20.989007028293386	28.23379587913738	37.99579503814501
5	10.467229072031149	40.64989064340135	33.26555627658808	15.617324007979427
6	32.52130229427813	25.376230365233692	23.6333481558042	18.46911918468398
7	24.564191455243524	32.99883880553238	25.169310808244237	17.267658930979852
8	34.113327145323645	26.5270837641695	24.87435615041673	14.485232940090127
9	27.42974224795948	21.462582907273717	15.942726044066507	35.16494880070029
10	16.25865295342919	31.18406557724039	31.955194658379714	20.602086810950706
11	32.35683222044759	25.30764716069814	13.694748590451633	28.64077202840264
12	19.39617739394012	26.96633267979359	29.90148790550537	23.736002020760914
13	37.15087023870229	23.02211916834236	21.532514888826885	18.29449570412846
14	30.99210530113874	16.86511659233815	22.03323374755245	30.10954435897066
15	31.347275504191067	17.86682380671774	36.428992339422514	14.356908349668682
16	28.133207623408985	23.57830734152372	30.348604523633142	17.93988051143415
17	25.50970336659194	21.662032270789837	34.751474507615484	18.076789855002744
18	30.991590412685582	16.267115049697388	36.78448748664954	15.956807050967486
19	18.524532586626638	42.255083090364984	24.890543960432243	14.329840362576137
20	18.782952971059114	17.931226908866996	46.01677955665024	17.269040563423648
21	19.793693903914665	33.00849424936191	19.908923171667553	27.288888675055873
22	16.71619166776945	32.86045980053656	24.228673171083813	26.19467536061018
23	16.50627453999538	30.9341077065209	22.05351643698514	30.506101316498572
24	14.542712044630592	38.61465217150734	23.578049408317476	23.264586375544592
25	28.251587093483955	23.88422336461413	18.631118338597584	29.233071203304327
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	375.0
1	375.0
2	757.5
3	1140.0
4	1140.0
5	1140.0
6	3330.5
7	5521.0
8	5521.0
9	5521.0
10	4691.0
11	3861.0
12	3861.0
13	3861.0
14	2507.5
15	1154.0
16	1154.0
17	1154.0
18	1744.0
19	2334.0
20	2334.0
21	2334.0
22	3184.5
23	4035.0
24	4035.0
25	4035.0
26	5907.5
27	7780.0
28	7780.0
29	7780.0
30	8539.5
31	9299.0
32	9299.0
33	9299.0
34	8665.5
35	8032.0
36	8032.0
37	8032.0
38	10992.5
39	13953.0
40	13953.0
41	13953.0
42	32641.5
43	51330.0
44	51330.0
45	51330.0
46	123928.5
47	196527.0
48	196527.0
49	196527.0
50	133805.5
51	71084.0
52	71084.0
53	71084.0
54	42884.0
55	14684.0
56	14684.0
57	14684.0
58	11389.5
59	8095.0
60	8095.0
61	8095.0
62	7104.0
63	6113.0
64	6113.0
65	6113.0
66	5374.5
67	4636.0
68	4636.0
69	4636.0
70	3784.5
71	2933.0
72	2933.0
73	2933.0
74	2336.5
75	1740.0
76	1740.0
77	1740.0
78	1236.0
79	732.0
80	732.0
81	732.0
82	513.0
83	294.0
84	294.0
85	294.0
86	197.5
87	101.0
88	101.0
89	101.0
90	62.0
91	23.0
92	23.0
93	23.0
94	19.5
95	16.0
96	16.0
97	16.0
98	226.0
99	436.0
100	436.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007447841087096495
2	0.002883035259521224
3	0.004324552889281836
4	0.012733405729552073
5	0.037959964250362785
6	0.04636881709063302
7	0.06655006390728159
8	0.08889358716857107
9	0.096581681193961
10	0.11387989275108835
11	0.11820444564037019
12	0.13045734549333537
13	0.11532141038084896
14	0.12156798677647826
15	0.11123711042986056
16	0.10955533986180652
17	0.11916545739354392
18	0.12300950440623888
19	0.12877557492528136
20	0.11628242213402269
21	0.1285353219869879
22	0.14727505117387585
23	0.1393467042101925
24	0.131898863123096
25	0.12757431023381416
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	416228.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.362493633297136
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.85416699247767	11.499466638476989
2	12.453278701343384	3.826268295261251
3	4.460222385562141	2.0556041400386325
4	2.2207278357286957	1.3646366895067126
5	1.2808282376491562	0.9838357823116176
6	0.8585771702922915	0.791393178738576
7	0.5536180660901115	0.5953467810911327
8	0.44883724567192657	0.5516207463217275
9	0.3159063540966173	0.4367798418174655
>10	2.0236773376288255	5.962837675504772
>50	0.20017828378399513	2.15122480947942
>100	0.21581721220461972	7.087461679656343
>500	0.03440564252537416	3.7301671199438773
>1k	0.05942792799837355	19.943876913614652
>5k	0.014075035578562156	16.066674995435193
>10k+	0.006255571368249848	22.95280471280164
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	38229	9.184629578019738	No Hit
TCCATGTACTCTGCGTTGATACCAC	31307	7.52159873915258	No Hit
GAGTACATGGAAGCAGTGGTATCAA	14353	3.448350423325677	No Hit
CATGTACTCTGCGTTGATACCACTG	11647	2.7982259723036416	No Hit
CCCATGTACTCTGCGTTGATACCAC	9909	2.3806663655496507	No Hit
GCTTCCATGTACTCTGCGTTGATAC	9296	2.233391314375775	No Hit
GTACATGGGAAGCAGTGGTATCAAC	8921	2.1432964625157367	No Hit
GCGTTGATACCACTGCTTCCATGTA	8628	2.07290235159576	No Hit
CATGGAAGCAGTGGTATCAACGCAG	8066	1.9378802002748492	No Hit
GTATCAACGCAGAGTACATGGAAGC	6457	1.5513132225607118	No Hit
CAGTGGTATCAACGCAGAGTACATG	5284	1.2694965259425124	No Hit
ACTCTGCGTTGATACCACTGCTTCC	5259	1.2634902024851764	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	5054	1.2142383501350222	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4762	1.144084492153339	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4703	1.1299095687940264	No Hit
TATCAACGCAGAGTACATGGAAGCA	4038	0.9701413648288918	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3589	0.8622677955351394	No Hit
GTATCAACGCAGAGTACATGGGAAG	3151	0.7570370085626148	No Hit
GAGTACATGGGAAGCAGTGGTATCA	2784	0.6688641802089239	No Hit
GGTATCAACGCAGAGTACATGGAAG	2764	0.6640591214430552	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2758	0.6626176038132947	No Hit
ATACCACTGCTTCCATGTACTCTGC	2731	0.6561307744793718	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	2680	0.6438778746264067	No Hit
GTACTCTGCGTTGATACCACTGCTT	2672	0.6419558511200592	No Hit
GCGTTGATACCACTGCTTCCCATGT	2563	0.6157682808460747	No Hit
GTGGTATCAACGCAGAGTACATGGA	2434	0.5847756518062216	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	2306	0.5540232757046619	No Hit
GCTTCCCATGTACTCTGCGTTGATA	2265	0.544172905234631	No Hit
GTACATGGGGTGGTATCAACGCAAA	2217	0.532640764196546	No Hit
ACATGGAAGCAGTGGTATCAACGCA	2128	0.5112582526884304	No Hit
CCACTGCTTCCATGTACTCTGCGTT	2114	0.5078947115523224	No Hit
AAAAAGTACTCTGCGTTGATACCAC	2065	0.496122317575944	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1989	0.47786309426564283	No Hit
TATCAACGCAGAGTACATGGGAAGC	1937	0.46536994147438426	No Hit
TGGAAGCAGTGGTATCAACGCAGAG	1856	0.44590945347261596	No Hit
CTGCTTCCATGTACTCTGCGTTGAT	1813	0.43557857712599823	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	1759	0.42260491845815273	No Hit
GTTGATACCACTGCTTCCATGTACT	1633	0.3923330482331799	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1604	0.38536571302267025	No Hit
GCAGAGTACATGGAAGCAGTGGTAT	1540	0.3699895249718904	No Hit
CCCCATGTACTCTGCGTTGATACCA	1517	0.36446370739114137	No Hit
GATACCACTGCTTCCATGTACTCTG	1487	0.35725611924233835	No Hit
GGTATCAACGCAGAGTACATGGGAA	1449	0.34812650758718783	No Hit
GTACATGGGTGGTATCAACGCAAAA	1404	0.33731512536398317	No Hit
GCAGTGGTATCAACGCAGAGTACAT	1329	0.31929615499197556	No Hit
CTTCCATGTACTCTGCGTTGATACC	1323	0.3178546373622149	No Hit
CCATGTACTCTGCGTTGATACCACT	1318	0.3166533726707478	No Hit
ATCAACGCAGAGTACATGGAAGCAG	1162	0.27917391429697186	No Hit
GTACATGGGAGTGGTATCAACGCAA	1095	0.2630769674313117	No Hit
TCCATGTACTCTGCGTTGATACCAA	1037	0.24914229701029242	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	1036	0.24890204407199898	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	945	0.2270390266872964	No Hit
GTGGTATCAACGCAGAGTACATGGG	944	0.22679877374900295	No Hit
AAAGTACTCTGCGTTGATACCACTG	936	0.2248767502426555	No Hit
CACTGCTTCCATGTACTCTGCGTTG	904	0.21718865621726552	No Hit
GTACATGGAAGCAGTGGTATCAACC	841	0.2020527211047791	No Hit
GTATCAACGCAGAGTACATGGAATC	839	0.20157221522819224	No Hit
TGATACCACTGCTTCCATGTACTCT	825	0.19820867409208412	No Hit
GTATCAACGCAGAGTACATGGGGTG	810	0.1946048800176826	No Hit
ATACCACTGCTTCCCATGTACTCTG	679	0.1631317451012426	No Hit
TATCAACGCAGAGTACATGGGGTGG	661	0.15880719221196077	No Hit
TCCATGTACTCTGCGTTGATACAAC	660	0.1585669392736673	No Hit
GATACCACTGCTTCCCATGTACTCT	654	0.1571254216439067	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	642	0.15424238638438548	No Hit
CTGCGTTGATACCACTGCTTCCATG	641	0.15400213344609204	No Hit
CATGGGAAGCAGTGGTATCAACGCA	628	0.15087884524827738	No Hit
CTCTGCGTTGATACCACTGCTTCCA	627	0.15063859230998394	No Hit
GTACATGGAAGCAGTGGTATCAAAG	599	0.14391151003776775	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	575	0.13814543951872532	No Hit
GTATCAACGCAGAGTACATGGGAGT	572	0.137424680703845	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	527	0.12661329848064043	No Hit
TCCATGTACTCTGCGTTGATACAAA	511	0.12276925146794546	No Hit
AGTGGTATCAACGCAGAGTACATGG	506	0.12156798677647826	No Hit
ATCAACGCAGAGTACATGGGAAGCA	500	0.12012646914671765	No Hit
TACCACTGCTTCCATGTACTCTGCG	493	0.11844469857866362	No Hit
TACCACTGCTTCCCATGTACTCTGC	465	0.11171761630644744	No Hit
GTACATGGTAAGCAGTGGTATCAAC	455	0.10931508692351306	No Hit
CCACTGCTTCCCATGTACTCTGCGT	451	0.10835407517033933	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	446	0.10715281047887215	No Hit
GTTGATACCACTGCTTCCCATGTAC	441	0.10595154578740498	No Hit
GTACATGGAAAGCAGTGGTATCAAC	434	0.10426977521935094	No Hit
GAGTACATGGGGTGGTATCAACGCA	419	0.1006659811449494	No Hit
ACATGGGAAGCAGTGGTATCAACGC	418	0.10042572820665598	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	2.4025293829343532E-4	0.0
6	0.0	0.0	0.0	4.8050587658687063E-4	0.0
7	0.0	0.0	0.0	7.20758814880306E-4	0.0
8	0.0	0.0	0.0	7.20758814880306E-4	0.0
9	0.0	0.0	0.0	7.20758814880306E-4	0.0
10	0.0	0.0	0.0	9.610117531737413E-4	0.0
11	0.0	0.0	0.0	9.610117531737413E-4	0.0
12	0.0	0.0	0.0	9.610117531737413E-4	0.0
13	0.0	0.0	0.0	9.610117531737413E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTAGAG	25	0.0060444474	18.9893	3
ATACAAA	90	7.2759576E-12	16.88953	19
ATAACAA	35	0.0021660554	16.286333	19
ATACCAA	155	0.0	15.323163	19
TGATAAC	120	3.6379788E-12	14.2522545	17
AAAAGTA	320	0.0	14.240263	2
GTACATA	40	0.0053029126	14.23684	1
AAAAAGT	320	0.0	14.23684	1
GATAACA	130	1.4551915E-11	13.155929	18
GGAGTGG	260	0.0	10.963274	8
AAGTACT	420	0.0	10.852334	4
ACATGGC	70	0.0014944338	10.851028	3
TTGATAA	185	5.456968E-12	10.785491	16
GATACAA	300	0.0	10.768372	18
ATGGGGG	80	3.7568464E-4	10.687906	5
GGGGTGG	490	0.0	10.664953	7
AAAGTAC	420	0.0	10.624967	3
ATCAACC	170	1.364242E-10	10.618051	19
AGTACTC	445	0.0	10.24758	5
GGTGTGG	85	6.561766E-4	10.060415	8
>>END_MODULE