FastQCFastQC Report
Thu 2 Feb 2017
SRR4062348_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062348_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416228
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA135733.260953131456798No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC117862.831621130726429No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA45391.090508086913903No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC42061.0105038584621888No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG33190.7973995021959118No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA29940.7193172972505454No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC29440.7073046503358736No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA26820.6443583805029935No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG26610.6393130687988314No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT26330.6325859865266151No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA23070.5542635286429554No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATA20700.49732358226741114No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA20010.48074612952516405No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG16990.4081897421605466No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC15960.3834436895163228No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA15100.36278193682308735No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG14750.35437308398281714No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG13670.3284257666471261No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTATA13340.32049741968344275No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA12540.3012771846199679No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT11690.2808556848650259No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTCTCTTATA11690.2808556848650259No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10340.24842153819541213No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10090.24241521473807623No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG9670.23232459132975194No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9320.22391573848948174No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC9000.21622764446409182No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA8540.20517600930259378No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTATGCGTTGATA8390.20157221522819224No Hit
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA8290.19916968584525788No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC8060.19364386826450888No Hit
GATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC7960.19124133888157452No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTG7840.1883583036220533No Hit
ACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAA7260.17442363320103405No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC6990.1679368038671113No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA6960.16721604505223098No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA6810.16361225097782944No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6770.1626512392246557No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA6650.1597682039651345No Hit
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG6630.15928769808854762No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT6590.1583266863353739No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA6540.1571254216439067No Hit
TGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC6420.15424238638438548No Hit
GTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT6200.1489568217419299No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT6130.14727505117387585No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTTCGTTGATA5840.14030771596336625No Hit
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT5800.1393467042101925No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAG5390.12949633374016165No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG5370.12901582786357477No Hit
TGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTA5280.12685355141893387No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGGTGATA5050.12132773383818483No Hit
GCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTG5030.12084722796159796No Hit
ATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAA4900.11772393976378331No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAATCAGTGGTATA4850.11652267507231613No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA4840.11628242213402269No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTA4810.11556166331914239No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4730.1136396398127949No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG4590.11027609867668682No Hit
CTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGA4470.1073930634171656No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG4410.10595154578740498No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTAG207.851005E-443.99963814
ATGGTCG302.5239533E-643.99963834
ATACATG1150.040.183241
GTTCGTT357.2794264E-637.71397840
TGGTCGC357.2794264E-637.71397835
GTTCGAT301.299732E-436.66636711
GTATAAC250.002348350635.208171
TCTAGAT250.002349735535.203942
TATAACG250.002349735535.203942
CAGGTTA250.00235112135.1997122
ATTAGTC250.00235112135.1997116
ACTACTC250.00235112135.1997134
ACTAACA250.00235112135.1997111
ATAACGC250.00235112135.199713
AACTACT250.00235112135.1997133
GAATGAG451.0351123E-634.22194337
CTCCGAT551.3701538E-731.99973740
GGTCGCA353.2144197E-431.42831636
TGTTCGT353.2144197E-431.42831639
GCGATCA353.2144197E-431.42831642
Produced by FastQC (version 0.11.5)