FastQCFastQC Report
Thu 2 Feb 2017
SRR4062347_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062347_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1637403
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24350.14871109922236617No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4600.014.0344911
GTATCAA14650.013.4146161
TAGACCG500.001499588413.300025
GTACTAG1052.0116204E-812.658561
AAGACGG1750.012.4857345
CGAACGA1001.4391117E-712.35228516
GACTTAT550.003083313312.083171
AAAGCGT951.039085E-612.00075210
GAAAGCG1052.7177884E-711.7626449
CGCGTAA658.019589E-411.69304210
ACCGTCG1552.5465852E-1111.6462498
CCGTCGT1552.5465852E-1111.6458929
GTTGTAT907.514487E-611.6036791
CGCATCG1406.7848305E-1011.53679213
GCATCGC1501.7644197E-1011.40106414
GTTTACG1001.928136E-611.4007159
TTACGAC1001.928136E-611.40071511
CGTCGTA1604.5474735E-1111.28195910
TAACGAA1104.962185E-711.22832113
TAAGACT1104.970043E-711.2269484
Produced by FastQC (version 0.11.5)