##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062347_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1637403
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.11800149382895	32.0	32.0	32.0	32.0	32.0
2	30.715652774546033	32.0	32.0	32.0	32.0	32.0
3	30.775840156638285	32.0	32.0	32.0	32.0	32.0
4	30.592981080405984	32.0	32.0	32.0	32.0	32.0
5	30.647989529761457	32.0	32.0	32.0	32.0	32.0
6	33.975315178975485	36.0	36.0	36.0	32.0	36.0
7	34.005931954442495	36.0	36.0	36.0	32.0	36.0
8	33.92453110199505	36.0	36.0	36.0	32.0	36.0
9	34.1269895071647	36.0	36.0	36.0	32.0	36.0
10	33.75183751342828	36.0	36.0	36.0	32.0	36.0
11	34.20303981365614	36.0	36.0	36.0	32.0	36.0
12	33.92789618682755	36.0	36.0	36.0	32.0	36.0
13	34.081803319036304	36.0	36.0	36.0	32.0	36.0
14	33.91577699564493	36.0	36.0	36.0	32.0	36.0
15	33.818424663934294	36.0	36.0	36.0	32.0	36.0
16	33.85098414990079	36.0	36.0	36.0	32.0	36.0
17	33.784144159989935	36.0	36.0	36.0	32.0	36.0
18	33.807327823388626	36.0	36.0	36.0	32.0	36.0
19	33.78519399317089	36.0	36.0	36.0	32.0	36.0
20	33.71157925080142	36.0	36.0	36.0	32.0	36.0
21	33.70932201785388	36.0	36.0	36.0	32.0	36.0
22	33.65368635577192	36.0	36.0	36.0	32.0	36.0
23	33.635779951545224	36.0	36.0	36.0	27.0	36.0
24	33.62254313690643	36.0	36.0	36.0	27.0	36.0
25	32.90253834883654	36.0	32.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	13.0
4	69.0
5	139.0
6	281.0
7	79.0
8	236.0
9	291.0
10	205.0
11	65.0
12	106.0
13	101.0
14	387.0
15	683.0
16	1043.0
17	1405.0
18	1721.0
19	2271.0
20	2909.0
21	3814.0
22	5022.0
23	6972.0
24	9165.0
25	12615.0
26	17698.0
27	24115.0
28	33792.0
29	46767.0
30	65730.0
31	97977.0
32	151084.0
33	228163.0
34	454339.0
35	468144.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.20775597382712	18.026076382166135	11.249967170901263	26.516200473105485
2	16.719455410713937	20.12460423710595	37.20207848808803	25.953861864092094
3	18.543801509541822	23.860606186458586	29.185289601653437	28.41030270234615
4	12.745062708322772	15.75987534686104	36.460126937550655	35.034935007265524
5	14.387505116597326	36.404574696211576	34.24552335917597	14.962396828015127
6	33.4636814503835	35.705954200152384	17.239801227268256	13.590563122195862
7	29.532554012232502	30.8793763475557	21.38207268122638	18.205996958985416
8	27.423082589063313	33.42292488185713	20.008215445159152	19.145777083920407
9	27.101726226936673	14.658257438767789	19.208202359425414	39.031813974870126
10	15.535919993543132	26.99734877111628	32.550866174085954	24.91586506125464
11	36.1588125197574	21.496970582642348	23.1983847999007	19.14583209769955
12	23.93121886607382	24.089785564987885	29.443506103564292	22.535489465374
13	28.76648168212824	20.027062854794885	25.679689773836223	25.526765689240648
14	22.846130939095776	20.16294773479535	25.632715756852726	31.35820556925615
15	24.69754767059583	27.62042301444104	22.50684327628616	25.175186038676966
16	25.18594196226318	26.231741309410484	24.100707328421347	24.48160939990499
17	23.579580718044042	26.143394795816356	25.725519210720215	24.551505275419387
18	24.110598943077598	25.47792044830534	26.517353853755303	23.89412675486176
19	24.982449445842782	25.441454531222863	25.803534082320052	23.772561940614306
20	24.849399432083608	25.409917258348152	25.429667150121187	24.311016159447053
21	25.61237137862061	24.760307290975543	25.088866236211437	24.5384550941924
22	24.949418465257153	25.183426871455623	25.241959554344263	24.62519510894296
23	24.25651052098234	24.970385179196423	25.692393593046802	25.08071070677444
24	24.217451951271567	25.585301972093855	25.595025154913543	24.60222092172104
25	24.2757571421408	25.131138498643057	25.82557020327843	24.76753415593771
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	99.0
1	99.0
2	335.5
3	572.0
4	572.0
5	572.0
6	1710.5
7	2849.0
8	2849.0
9	2849.0
10	3258.0
11	3667.0
12	3667.0
13	3667.0
14	4929.0
15	6191.0
16	6191.0
17	6191.0
18	10117.0
19	14043.0
20	14043.0
21	14043.0
22	21608.0
23	29173.0
24	29173.0
25	29173.0
26	42265.5
27	55358.0
28	55358.0
29	55358.0
30	67908.5
31	80459.0
32	80459.0
33	80459.0
34	96743.0
35	113027.0
36	113027.0
37	113027.0
38	126514.0
39	140001.0
40	140001.0
41	140001.0
42	156245.0
43	172489.0
44	172489.0
45	172489.0
46	191014.0
47	209539.0
48	209539.0
49	209539.0
50	211764.5
51	213990.0
52	213990.0
53	213990.0
54	200575.5
55	187161.0
56	187161.0
57	187161.0
58	169478.5
59	151796.0
60	151796.0
61	151796.0
62	132403.0
63	113010.0
64	113010.0
65	113010.0
66	91873.5
67	70737.0
68	70737.0
69	70737.0
70	53620.5
71	36504.0
72	36504.0
73	36504.0
74	27713.0
75	18922.0
76	18922.0
77	18922.0
78	14859.0
79	10796.0
80	10796.0
81	10796.0
82	7475.5
83	4155.0
84	4155.0
85	4155.0
86	2904.5
87	1654.0
88	1654.0
89	1654.0
90	1104.0
91	554.0
92	554.0
93	554.0
94	320.5
95	87.0
96	87.0
97	87.0
98	328.5
99	570.0
100	570.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008305835521249198
2	0.0036032668805419314
3	0.005191147200780748
4	0.013435910402020761
5	0.03499443936526316
6	0.04641496320698081
7	0.06760705825016809
8	0.0891655872134105
9	0.09655533793452192
10	0.11817493921777351
11	0.11848030081781943
12	0.13002296929955545
13	0.11915209633792047
14	0.12409895425866448
15	0.11335022593704787
16	0.11066304385664372
17	0.12141177217826034
18	0.12758007649918804
19	0.1299008246595371
20	0.11915209633792047
21	0.12703042561910538
22	0.14773394210221918
23	0.1369241414605934
24	0.13045047553961975
25	0.12880152289937175
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1637403.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.19330354433119
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.6255633565709	50.09266659375531
2	8.90697875540059	10.544669942626728
3	2.4405384799207357	4.3339060516070615
4	1.1162911335604804	2.6430783965076423
5	0.6599009902308589	1.9530859811969978
6	0.4331124652536438	1.5382414574755519
7	0.30556354391350343	1.2661120924867506
8	0.21495448169865733	1.0179092706722406
9	0.17731692083081757	0.9446376886456255
>10	0.9534589337448302	10.366568030678067
>50	0.0824141459074503	3.4069846511379267
>100	0.07770699618243349	9.1974194831303
>500	0.005786476999447956	2.3091399267788577
>1k	4.1331978567485405E-4	0.38558043330103353
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2435	0.14871109922236617	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.107232000918528E-5
2	0.0	0.0	0.0	6.107232000918528E-5	6.107232000918528E-5
3	0.0	0.0	0.0	6.107232000918528E-5	6.107232000918528E-5
4	0.0	0.0	0.0	6.107232000918528E-5	6.107232000918528E-5
5	0.0	0.0	0.0	6.107232000918528E-5	6.107232000918528E-5
6	0.0	0.0	0.0	6.107232000918528E-5	6.107232000918528E-5
7	0.0	0.0	0.0	1.2214464001837055E-4	6.107232000918528E-5
8	0.0	0.0	0.0	1.2214464001837055E-4	1.2214464001837055E-4
9	0.0	0.0	0.0	1.8321696002755584E-4	1.2214464001837055E-4
10	0.0	0.0	0.0	1.8321696002755584E-4	1.2214464001837055E-4
11	6.107232000918528E-5	0.0	0.0	1.8321696002755584E-4	1.2214464001837055E-4
12	6.107232000918528E-5	0.0	0.0	1.8321696002755584E-4	1.8321696002755584E-4
13	6.107232000918528E-5	0.0	0.0	2.442892800367411E-4	1.8321696002755584E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	460	0.0	14.034491	1
GTATCAA	1465	0.0	13.414616	1
TAGACCG	50	0.0014995884	13.30002	5
GTACTAG	105	2.0116204E-8	12.65856	1
AAGACGG	175	0.0	12.485734	5
CGAACGA	100	1.4391117E-7	12.352285	16
GACTTAT	55	0.0030833133	12.08317	1
AAAGCGT	95	1.039085E-6	12.000752	10
GAAAGCG	105	2.7177884E-7	11.762644	9
CGCGTAA	65	8.019589E-4	11.693042	10
ACCGTCG	155	2.5465852E-11	11.646249	8
CCGTCGT	155	2.5465852E-11	11.645892	9
GTTGTAT	90	7.514487E-6	11.603679	1
CGCATCG	140	6.7848305E-10	11.536792	13
GCATCGC	150	1.7644197E-10	11.401064	14
GTTTACG	100	1.928136E-6	11.400715	9
TTACGAC	100	1.928136E-6	11.400715	11
CGTCGTA	160	4.5474735E-11	11.281959	10
TAACGAA	110	4.962185E-7	11.228321	13
TAAGACT	110	4.970043E-7	11.226948	4
>>END_MODULE