FastQCFastQC Report
Thu 2 Feb 2017
SRR4062347_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062347_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1637403
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12050.025.7473321
GGTATCA5750.024.8740021
GTATTAG5550.021.4092241
TATACCG755.4743607E-520.5332325
TTAACGG4250.020.18813735
TAATACT3750.019.946574
GTAATAC1605.2750693E-1119.2499073
TAACGGC4500.019.06657636
TATTAGA5350.018.9163722
CGGATCG1303.5897756E-818.61529526
CTAATAC5750.017.9825213
TAGGACG3850.017.7142014
GTCTAAA1751.9099389E-1017.603141
ACCGTCG3000.017.5999158
CGCAATA3050.017.31139236
ATCGATT1408.3779014E-817.28563129
CGTCGTA2950.017.1524610
TACCGTC3100.017.0321757
ATACCGT3250.016.9229956
CGGCAAA3150.016.76182436