FastQCFastQC Report
Thu 2 Feb 2017
SRR4062346_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062346_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7071869
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA54600.012.8684471
GGTATCA20700.012.5680311
TATGCCG1603.6379788E-1211.87455
CGTCTTA6900.011.84175915
GCGTTAT6750.011.2531281
CGTTATT6600.011.0785522
CGCATCG9400.011.01650713
GCTAGTA1158.8806337E-710.7344982
AACCGCG6150.010.6591447
GATATAC8600.010.4884721
CGGTCCA14400.010.424510
CGAACGA5800.010.32065116
CGTCGTA11800.010.14493410
ATACCGT12100.010.1286056
CGACCAT10900.010.11095410
CCGTCGT10950.010.0646439
AGAACCG8500.010.0584015
ACGGTAT11050.09.9735619
CGCGTAA5350.09.94477410
ACCGTCG11500.09.8310658
Produced by FastQC (version 0.11.5)