##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062346_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7071869
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.286605280725645	32.0	32.0	32.0	32.0	32.0
2	30.93296411457848	32.0	32.0	32.0	32.0	32.0
3	30.90869895921432	32.0	32.0	32.0	32.0	32.0
4	30.9983888276211	32.0	32.0	32.0	32.0	32.0
5	30.777096266913315	32.0	32.0	32.0	32.0	32.0
6	34.45713629593535	36.0	36.0	36.0	32.0	36.0
7	34.36795534532667	36.0	36.0	36.0	32.0	36.0
8	34.372006183938076	36.0	36.0	36.0	32.0	36.0
9	34.53956287934632	36.0	36.0	36.0	32.0	36.0
10	34.200284677218995	36.0	36.0	36.0	32.0	36.0
11	34.487859857132534	36.0	36.0	36.0	32.0	36.0
12	34.273527266978505	36.0	36.0	36.0	32.0	36.0
13	34.394157895175944	36.0	36.0	36.0	32.0	36.0
14	34.29864622775111	36.0	36.0	36.0	32.0	36.0
15	34.23030517109409	36.0	36.0	36.0	32.0	36.0
16	34.23937292956077	36.0	36.0	36.0	32.0	36.0
17	34.16875567123769	36.0	36.0	36.0	32.0	36.0
18	34.17542024604811	36.0	36.0	36.0	32.0	36.0
19	34.16934561429235	36.0	36.0	36.0	32.0	36.0
20	34.168351393386956	36.0	36.0	36.0	32.0	36.0
21	34.15237046953217	36.0	36.0	36.0	32.0	36.0
22	34.10651851724063	36.0	36.0	36.0	32.0	36.0
23	34.066303264384565	36.0	36.0	36.0	32.0	36.0
24	34.02970982635566	36.0	36.0	36.0	32.0	36.0
25	33.593761847115665	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3.0
3	19.0
4	274.0
5	668.0
6	1179.0
7	277.0
8	1110.0
9	1261.0
10	848.0
11	287.0
12	553.0
13	382.0
14	831.0
15	1036.0
16	1756.0
17	2395.0
18	3161.0
19	4463.0
20	6848.0
21	10174.0
22	15840.0
23	24380.0
24	35933.0
25	51971.0
26	74632.0
27	98173.0
28	135192.0
29	182901.0
30	238907.0
31	330236.0
32	480028.0
33	704626.0
34	1607314.0
35	3054211.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.13289466669174	17.163872946852457	11.393896745948433	26.309335640507364
2	17.279849720516815	19.499283332937384	36.75939218487861	26.46147476166719
3	19.35687321511777	22.920238787987632	28.027595101211972	29.69529289568263
4	13.167522506908313	15.380502059942335	35.021593649061515	36.430381784087835
5	15.34136496192596	35.65996697873535	32.99208247847337	16.00658558086532
6	34.73928271357224	34.2773213825679	16.964749665313587	14.018646238546284
7	31.091155739487597	29.73174439370701	20.387604369145322	18.789495497660074
8	28.298963513067115	32.53642508390656	19.284468207786517	19.880143195239807
9	27.240957267696185	14.976695626868459	18.692642289443583	39.08970481599177
10	16.619718030697143	26.123111088396183	30.700188988493203	26.55698189241346
11	37.08417270739919	20.87173429866129	22.556103033440888	19.487989960498627
12	25.434117515625992	23.084586596315066	28.114526003864675	23.366769884194266
13	29.322369388808845	19.265586931157834	25.158534860538452	26.25350881949487
14	23.697655291772872	19.87005258805345	24.456156163426616	31.976135956747058
15	25.396060033140966	27.339273036404805	21.56374114937289	25.700925781081335
16	26.243326097004555	25.35333551815252	23.46664269500042	24.9366956898425
17	24.406172064308738	25.667570186808515	24.872821694110883	25.053436054771865
18	25.22046494663277	24.477251494993187	25.880918203476522	24.42136535489752
19	26.001829095532226	24.493101387368537	24.771246244428504	24.733823272670737
20	25.911588740429647	24.39388108932784	24.38358833041823	25.310941839824284
21	26.653030641129185	24.000009061736478	24.006791205131663	25.34016909200268
22	26.054236062999532	24.308057121719294	24.336182923644536	25.30152389163664
23	24.653763108318294	24.18270194768341	24.99658733422374	26.166947609774553
24	25.20696129071631	24.66376799322488	24.73718588716923	25.392084828889583
25	25.20973636035787	24.287577431233476	24.9003441671338	25.602342041274852
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	407.0
1	407.0
2	1178.5
3	1950.0
4	1950.0
5	1950.0
6	4512.5
7	7075.0
8	7075.0
9	7075.0
10	7868.5
11	8662.0
12	8662.0
13	8662.0
14	11988.0
15	15314.0
16	15314.0
17	15314.0
18	25859.5
19	36405.0
20	36405.0
21	36405.0
22	61950.5
23	87496.0
24	87496.0
25	87496.0
26	136582.5
27	185669.0
28	185669.0
29	185669.0
30	239125.5
31	292582.0
32	292582.0
33	292582.0
34	363933.0
35	435284.0
36	435284.0
37	435284.0
38	497488.5
39	559693.0
40	559693.0
41	559693.0
42	637020.5
43	714348.0
44	714348.0
45	714348.0
46	803156.5
47	891965.0
48	891965.0
49	891965.0
50	916862.0
51	941759.0
52	941759.0
53	941759.0
54	892509.5
55	843260.0
56	843260.0
57	843260.0
58	783772.5
59	724285.0
60	724285.0
61	724285.0
62	642031.5
63	559778.0
64	559778.0
65	559778.0
66	460702.0
67	361626.0
68	361626.0
69	361626.0
70	275784.0
71	189942.0
72	189942.0
73	189942.0
74	146833.5
75	103725.0
76	103725.0
77	103725.0
78	84699.0
79	65673.0
80	65673.0
81	65673.0
82	45753.5
83	25834.0
84	25834.0
85	25834.0
86	18735.5
87	11637.0
88	11637.0
89	11637.0
90	7938.5
91	4240.0
92	4240.0
93	4240.0
94	2503.5
95	767.0
96	767.0
97	767.0
98	1630.0
99	2493.0
100	2493.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008371195789967263
2	0.003464430690104695
3	0.004581532830995597
4	0.014706154766158707
5	0.03513922557106191
6	0.04683344671684388
7	0.06739378232260808
8	0.0886752851332512
9	0.09737171319208543
10	0.11985516134419345
11	0.12118437148651932
12	0.13345835450289026
13	0.1215520253556733
14	0.12525684511407098
15	0.11459488290860591
16	0.11275661356283607
17	0.12378622963745511
18	0.13143625822254343
19	0.13238367396228634
20	0.12232975469426823
21	0.13016361021393355
22	0.15182690742715962
23	0.14057104281767663
24	0.13515521851437012
25	0.13167664729083642
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	7071869.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.30907127465483
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.53895423171066	34.014491666580106
2	11.584044928504703	10.033884549148281
3	3.548813155213995	4.610874056387869
4	1.6859213629455791	2.9206275388509324
5	0.9988782281282043	2.163024418835266
6	0.6586822927398128	1.7116151018172967
7	0.4465795429828339	1.3538661679792434
8	0.33290955844167713	1.153440303565157
9	0.25885028879217037	1.0089509046088585
>10	1.6645691502719322	13.934979630674915
>50	0.15853082401570748	4.736594193215295
>100	0.09854086716148198	8.32399391694895
>500	0.01319575328071695	4.003784560863945
>1k	0.011072542662201226	8.871249707078038
>5k	4.572731481391268E-4	1.1586232834461856
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4140533428998755E-5
2	0.0	0.0	0.0	0.0	1.4140533428998755E-5
3	0.0	0.0	0.0	0.0	1.4140533428998755E-5
4	0.0	0.0	0.0	1.4140533428998755E-5	1.4140533428998755E-5
5	1.4140533428998755E-5	0.0	0.0	1.4140533428998755E-5	2.828106685799751E-5
6	1.4140533428998755E-5	0.0	0.0	2.828106685799751E-5	5.656213371599502E-5
7	1.4140533428998755E-5	0.0	0.0	4.2421600286996264E-5	5.656213371599502E-5
8	1.4140533428998755E-5	0.0	0.0	4.2421600286996264E-5	5.656213371599502E-5
9	1.4140533428998755E-5	0.0	0.0	7.070266714499378E-5	5.656213371599502E-5
10	1.4140533428998755E-5	0.0	0.0	1.272648008609888E-4	5.656213371599502E-5
11	1.4140533428998755E-5	0.0	0.0	1.272648008609888E-4	5.656213371599502E-5
12	1.4140533428998755E-5	0.0	0.0	1.272648008609888E-4	1.1312426743199004E-4
13	1.4140533428998755E-5	0.0	0.0	1.4140533428998756E-4	2.4038906829297883E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5460	0.0	12.868447	1
GGTATCA	2070	0.0	12.568031	1
TATGCCG	160	3.6379788E-12	11.8745	5
CGTCTTA	690	0.0	11.841759	15
GCGTTAT	675	0.0	11.253128	1
CGTTATT	660	0.0	11.078552	2
CGCATCG	940	0.0	11.016507	13
GCTAGTA	115	8.8806337E-7	10.734498	2
AACCGCG	615	0.0	10.659144	7
GATATAC	860	0.0	10.488472	1
CGGTCCA	1440	0.0	10.4245	10
CGAACGA	580	0.0	10.320651	16
CGTCGTA	1180	0.0	10.144934	10
ATACCGT	1210	0.0	10.128605	6
CGACCAT	1090	0.0	10.110954	10
CCGTCGT	1095	0.0	10.064643	9
AGAACCG	850	0.0	10.058401	5
ACGGTAT	1105	0.0	9.973561	9
CGCGTAA	535	0.0	9.944774	10
ACCGTCG	1150	0.0	9.831065	8
>>END_MODULE