FastQCFastQC Report
Thu 2 Feb 2017
SRR4062346_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062346_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7071869
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA58800.028.7417551
GGTATCA24400.022.636711
TCAACGC92400.018.0000974
CAACGCA95000.017.4843035
AACGCAG97650.017.1449976
TATCAAC101650.016.6660582
ATCAACG99950.016.640413
ACCGTCG15650.015.7442698
ATACCGT16950.015.5753046
TGTAGGA39550.015.4649272
TAGGACG31150.015.396554
TACCGTC16500.015.3331987
GTCCTAA19750.014.81883051
CGCAATA23100.014.76177536
ATACGAA23250.014.57191640
CGTCGTA17700.014.54224510
GTACATG190850.014.239811
CCGTCGT17850.014.0502979
TATACTG9100.014.0220535
TACATGG193200.013.9615262
Produced by FastQC (version 0.11.5)