##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062346_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7071869
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.343205452476568	32.0	32.0	32.0	32.0	32.0
2	31.436078920579554	32.0	32.0	32.0	32.0	32.0
3	31.510672638308204	32.0	32.0	32.0	32.0	32.0
4	31.608378350899883	32.0	32.0	32.0	32.0	32.0
5	31.537853571665426	32.0	32.0	32.0	32.0	32.0
6	35.146784251801044	36.0	36.0	36.0	36.0	36.0
7	35.16234265651697	36.0	36.0	36.0	36.0	36.0
8	35.101368393560456	36.0	36.0	36.0	36.0	36.0
9	35.2380810504267	36.0	36.0	36.0	36.0	36.0
10	35.110436717648476	36.0	36.0	36.0	36.0	36.0
11	35.24286846942442	36.0	36.0	36.0	36.0	36.0
12	35.15556396760177	36.0	36.0	36.0	36.0	36.0
13	35.19909305446693	36.0	36.0	36.0	36.0	36.0
14	35.16211216582207	36.0	36.0	36.0	36.0	36.0
15	35.11608289124134	36.0	36.0	36.0	36.0	36.0
16	35.13458266831583	36.0	36.0	36.0	36.0	36.0
17	35.10580965229984	36.0	36.0	36.0	36.0	36.0
18	35.12015225395154	36.0	36.0	36.0	36.0	36.0
19	35.1105485692679	36.0	36.0	36.0	36.0	36.0
20	35.10697850879308	36.0	36.0	36.0	36.0	36.0
21	35.10715611389295	36.0	36.0	36.0	36.0	36.0
22	35.08562630331529	36.0	36.0	36.0	36.0	36.0
23	35.04650962850132	36.0	36.0	36.0	36.0	36.0
24	35.02327758050948	36.0	36.0	36.0	36.0	36.0
25	35.00006773315513	36.0	36.0	36.0	36.0	36.0
26	34.953002664500715	36.0	36.0	36.0	32.0	36.0
27	34.93609440446366	36.0	36.0	36.0	32.0	36.0
28	34.920944095542495	36.0	36.0	36.0	32.0	36.0
29	34.90212742911386	36.0	36.0	36.0	32.0	36.0
30	34.88843006000253	36.0	36.0	36.0	32.0	36.0
31	34.89210589166739	36.0	36.0	36.0	32.0	36.0
32	34.863096870148475	36.0	36.0	36.0	32.0	36.0
33	34.8532472815885	36.0	36.0	36.0	32.0	36.0
34	34.84621420447692	36.0	36.0	36.0	32.0	36.0
35	34.828845528671415	36.0	36.0	36.0	32.0	36.0
36	34.80139564236838	36.0	36.0	36.0	32.0	36.0
37	34.79354495961393	36.0	36.0	36.0	32.0	36.0
38	34.7669401115886	36.0	36.0	36.0	32.0	36.0
39	34.76393185450692	36.0	36.0	36.0	32.0	36.0
40	34.7700774717405	36.0	36.0	36.0	32.0	36.0
41	34.73871603108033	36.0	36.0	36.0	32.0	36.0
42	34.72828880172978	36.0	36.0	36.0	32.0	36.0
43	34.71974537424265	36.0	36.0	36.0	32.0	36.0
44	34.69338982382168	36.0	36.0	36.0	32.0	36.0
45	34.67022480195829	36.0	36.0	36.0	32.0	36.0
46	34.669487090329305	36.0	36.0	36.0	32.0	36.0
47	34.655228342040836	36.0	36.0	36.0	32.0	36.0
48	34.623839327340484	36.0	36.0	36.0	32.0	36.0
49	34.61624472964643	36.0	36.0	36.0	32.0	36.0
50	34.12120346120665	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	10.0
20	45.0
21	176.0
22	612.0
23	1558.0
24	4107.0
25	9692.0
26	19504.0
27	36711.0
28	61337.0
29	95282.0
30	141446.0
31	206648.0
32	311071.0
33	529478.0
34	1216167.0
35	4438024.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.49660783711961	17.780152205365514	11.925450202313488	26.797789755201386
2	16.6211533775923	19.91693206068646	36.45940408348312	27.00251047823811
3	18.667146692881452	23.183442036834563	28.39204698751498	29.757364282769
4	12.88119755798264	15.893051019972404	35.15541460249909	36.070336819545865
5	15.08430091111699	35.85725357751961	32.903677938604346	16.154767572759056
6	34.97580753838716	34.39065498030142	16.718470789248215	13.915066692063203
7	31.177679105107604	29.754278467058278	20.27829426090734	18.78974816692678
8	28.39651418833044	32.59886920966285	19.093144838110664	19.911471763896046
9	27.285361196594565	14.76768022710828	18.554246409258994	39.39271216703816
10	16.572761740920257	26.176220176024188	30.668427257348803	26.582590825706752
11	37.403577470114335	20.69704628295575	22.40693372572371	19.492442521206204
12	25.489456888978008	22.97737698478295	28.028729038957028	23.504437087282017
13	29.446204390946722	19.143454156178517	25.135985974853327	26.274355478021437
14	23.778947262739173	19.73455673457752	24.357676874387803	32.128819128295504
15	25.497361447164817	27.33137449237252	21.453607243007472	25.717656817455186
16	26.34989420759915	25.331987908712676	23.339558467499895	24.978559416188283
17	24.59372479891808	25.660755310936896	24.65642392414226	25.089095966002763
18	25.402846121725386	24.4218466150886	25.732815469291076	24.442491793894938
19	26.113224099598	24.470900125553797	24.64569125926965	24.770184515578556
20	26.055120647738246	24.314279577294208	24.275732483166756	25.354867291800797
21	26.815626816616657	23.92812989041511	23.974864353397948	25.28137893957029
22	26.158332401236507	24.28104932373606	24.285560153899908	25.275058121127525
23	24.737222932155557	24.07954672237283	24.900093596190768	26.28313674928085
24	25.34987568350036	24.575582494528675	24.595633770930995	25.478908051039973
25	25.363676844127063	24.157758012768618	24.84456372141509	25.634001421689227
26	25.112880343230337	24.89158099506651	25.0712647533488	24.924273908354355
27	25.68534569856993	24.56195102030312	24.263585764951245	25.489117516175707
28	25.01389660922735	24.460591676684057	24.821344965524673	25.70416674856392
29	25.111692538422304	24.47972381841349	24.614143729189554	25.79443991397465
30	24.869649027718133	24.642806590450135	25.119201161673104	25.36834322015863
31	25.71948094626753	24.21997635985621	24.18329581614139	25.877246877734866
32	25.255176531126356	24.52437962298227	24.212665704073423	26.007778141817955
33	24.925984912899263	24.213033357942574	24.894592928686887	25.966388800471275
34	25.596924943038395	24.239108501585648	25.064505578369733	25.09946097700622
35	25.985605785401283	24.21731793957156	24.877101088835214	24.91997518619194
36	25.006430407576836	24.551529447165947	24.64060066723521	25.801439478022004
37	25.921379482566774	24.35361854129368	24.25269755421092	25.472304421928634
38	25.22951146295272	24.34741084711835	24.67885081016065	25.744226879768277
39	25.74340672882939	24.127058914694263	24.23827421011334	25.891260146363003
40	26.001824977244347	24.185727987891177	24.854094440946234	24.958352593918242
41	25.114379239773815	24.23756718344189	25.398208026760678	25.24984555002362
42	26.023177182722137	24.68536959607142	24.638366462953428	24.653086758253014
43	25.07870267393245	23.918726435684825	25.02675035411431	25.97582053626842
44	24.799427138709724	24.38014618200648	24.826661806093973	25.993764873189818
45	24.843319354473337	24.54653783886551	24.976735287375938	25.63340751928521
46	25.026350925032716	24.024846087124487	25.090054127218053	25.858748860624747
47	25.309136981545887	24.073564752720817	25.183710379086705	25.433587886646592
48	25.64487549189613	24.579145908952782	24.41015239394282	25.36582620520827
49	24.92785146331189	24.46590851725336	24.7485070778319	25.85773294160285
50	24.7020659045293	24.881990128206617	24.740160517747366	25.67578344951672
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	39.0
1	57.5
2	76.0
3	235.0
4	394.0
5	448.0
6	502.0
7	483.0
8	464.0
9	633.0
10	802.0
11	1183.5
12	1565.0
13	2800.0
14	4035.0
15	5118.0
16	6201.0
17	6860.5
18	7520.0
19	7997.5
20	8475.0
21	10723.0
22	12971.0
23	15102.5
24	17234.0
25	24687.0
26	32140.0
27	43994.5
28	55849.0
29	68247.5
30	80646.0
31	92618.5
32	104591.0
33	125646.0
34	146701.0
35	174405.5
36	202110.0
37	236383.0
38	270656.0
39	287726.5
40	304797.0
41	327526.0
42	350255.0
43	355914.5
44	361574.0
45	396860.5
46	432147.0
47	462689.0
48	493231.0
49	512856.5
50	532482.0
51	523577.0
52	514672.0
53	514757.0
54	514842.0
55	521688.0
56	528534.0
57	516848.0
58	505162.0
59	466044.0
60	426926.0
61	378703.5
62	330481.0
63	291223.0
64	251965.0
65	214924.0
66	177883.0
67	153016.0
68	128149.0
69	115202.0
70	102255.0
71	79090.5
72	55926.0
73	49438.5
74	42951.0
75	31178.5
76	19406.0
77	17689.5
78	15973.0
79	14055.0
80	12137.0
81	9595.5
82	7054.0
83	5946.0
84	4838.0
85	4022.0
86	3206.0
87	2318.0
88	1430.0
89	901.5
90	373.0
91	244.0
92	115.0
93	87.5
94	60.0
95	47.5
96	35.0
97	30.5
98	26.0
99	19.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016388878244209558
2	0.004355284296131617
3	4.666376031569589E-4
4	1.555458677189863E-4
5	0.0
6	5.373402703019527E-4
7	2.8281066857997514E-5
8	1.4140533428998757E-5
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.555458677189863E-4
47	5.656213371599503E-5
48	0.0
49	0.0
50	4.242160028699627E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	7071869.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.52956610598734
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.2577648390169	36.87829408489836
2	11.529752736103605	10.72948784240442
3	3.4478659655756703	4.812831221095111
4	1.5672462131402787	2.9169314511467563
5	0.9019246906746788	2.098308225868483
6	0.594269853235394	1.6590671052547
7	0.3970612278544724	1.2932560654705423
8	0.29894690813956043	1.1127895939568164
9	0.2328491913579905	0.9750934657815584
>10	1.53430623658669	13.713826098565141
>50	0.1365901900406254	4.362800582750581
>100	0.08124730470889581	7.3639019669412535
>500	0.010835186782204504	3.5450296377680353
>1k	0.008942682475463147	7.48613469171555
>5k	3.967743074894983E-4	1.0522479663825837
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.656213371599502E-5
2	0.0	0.0	0.0	0.0	5.656213371599502E-5
3	0.0	0.0	0.0	0.0	5.656213371599502E-5
4	0.0	0.0	0.0	1.4140533428998755E-5	5.656213371599502E-5
5	0.0	0.0	0.0	1.4140533428998755E-5	7.070266714499378E-5
6	0.0	0.0	0.0	2.828106685799751E-5	9.898373400299129E-5
7	0.0	0.0	0.0	2.828106685799751E-5	9.898373400299129E-5
8	0.0	0.0	0.0	2.828106685799751E-5	1.272648008609888E-4
9	0.0	0.0	0.0	2.828106685799751E-5	1.555458677189863E-4
10	0.0	0.0	0.0	5.656213371599502E-5	1.6968640114798506E-4
11	0.0	0.0	0.0	7.070266714499378E-5	1.6968640114798506E-4
12	0.0	0.0	0.0	7.070266714499378E-5	3.252322688669714E-4
13	0.0	0.0	0.0	7.070266714499378E-5	4.2421600286996267E-4
14	0.0	0.0	0.0	8.484320057399253E-5	4.2421600286996267E-4
15	0.0	1.4140533428998755E-5	0.0	1.1312426743199004E-4	5.373402703019527E-4
16	0.0	1.4140533428998755E-5	0.0	1.555458677189863E-4	5.656213371599502E-4
17	0.0	1.4140533428998755E-5	0.0	1.555458677189863E-4	5.656213371599502E-4
18	0.0	1.4140533428998755E-5	0.0	2.2624853486398008E-4	6.080429374469465E-4
19	0.0	1.4140533428998755E-5	0.0	2.2624853486398008E-4	6.221834708759452E-4
20	0.0	1.4140533428998755E-5	0.0	3.252322688669714E-4	6.928861380209391E-4
21	0.0	2.828106685799751E-5	0.0	4.100754694409639E-4	7.635888051659328E-4
22	0.0	2.828106685799751E-5	0.0	5.79761870588949E-4	7.918698720239303E-4
23	0.0	2.828106685799751E-5	0.0	8.342914723109266E-4	8.625725391689241E-4
24	0.0	2.828106685799751E-5	0.0	0.0014140533428998756	8.767130725979229E-4
25	0.0	2.828106685799751E-5	0.0	0.0016827234780508518	8.767130725979229E-4
26	0.0	2.828106685799751E-5	0.0	0.0021069394809208144	9.332752063139179E-4
27	0.0	2.828106685799751E-5	0.0	0.00295537148666074	9.474157397429166E-4
28	0.0	2.828106685799751E-5	0.0	0.005741056572173495	9.756968066009142E-4
29	0.0	2.828106685799751E-5	0.0	0.010874070206900044	9.898373400299128E-4
30	0.0	2.828106685799751E-5	0.0	0.02012197906946523	9.898373400299128E-4
31	0.0	2.828106685799751E-5	0.0	0.04708797631856586	0.001032258940316909
32	0.0	2.828106685799751E-5	0.0	0.0818736885539028	0.0010605400071749067
33	0.0	2.828106685799751E-5	0.0	0.11974203707676147	0.0010746805406039053
34	0.0	2.828106685799751E-5	0.0	0.16114551895686982	0.0011171021408909016
35	0.0	2.828106685799751E-5	0.0	0.2112171478289544	0.0011171021408909016
36	0.0	2.828106685799751E-5	0.0	0.28579432113349384	0.0011171021408909016
37	0.0	2.828106685799751E-5	0.0	0.3985650752297589	0.0011171021408909016
38	0.0	2.828106685799751E-5	0.0	0.5611953501966737	0.0011312426743199005
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5880	0.0	28.741755	1
GGTATCA	2440	0.0	22.63671	1
TCAACGC	9240	0.0	18.000097	4
CAACGCA	9500	0.0	17.484303	5
AACGCAG	9765	0.0	17.144997	6
TATCAAC	10165	0.0	16.666058	2
ATCAACG	9995	0.0	16.64041	3
ACCGTCG	1565	0.0	15.744269	8
ATACCGT	1695	0.0	15.575304	6
TGTAGGA	3955	0.0	15.464927	2
TAGGACG	3115	0.0	15.39655	4
TACCGTC	1650	0.0	15.333198	7
GTCCTAA	1975	0.0	14.8188305	1
CGCAATA	2310	0.0	14.761775	36
ATACGAA	2325	0.0	14.571916	40
CGTCGTA	1770	0.0	14.542245	10
GTACATG	19085	0.0	14.23981	1
CCGTCGT	1785	0.0	14.050297	9
TATACTG	910	0.0	14.022053	5
TACATGG	19320	0.0	13.961526	2
>>END_MODULE