FastQCFastQC Report
Thu 2 Feb 2017
SRR4062345_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062345_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3161094
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37810.11961048927997713No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATT1203.6379788E-1214.244112
GCGTTAT1350.014.0666971
GTATCAA28050.012.4907761
CGCATCG2850.011.66775613
TATGCCG1001.9344516E-611.3985365
GGTATCA10350.011.1921981
AGAACCG3050.010.5888045
CGTCGTA3500.010.58552910
TACCGTC3950.010.5820937
CCGTCGT3400.010.3378929
ATCCCCG1951.4551915E-1110.23221112
CAACGGA1951.4551915E-1110.23188614
GTATAGA2700.010.1983561
ACCGTCG3650.010.1505078
CGTCTTA3700.010.01524215
TGGCGAG19650.09.91422318
CGCGTAA1252.5727095E-69.879827510
ATACCGT3850.09.8701146
GCATCGC3200.09.79794514
CTTACAC3300.09.7849183
Produced by FastQC (version 0.11.5)