##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062345_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3161094
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.272057711665646	32.0	32.0	32.0	32.0	32.0
2	30.975808058855574	32.0	32.0	32.0	32.0	32.0
3	30.951727471565224	32.0	32.0	32.0	32.0	32.0
4	31.025261507566682	32.0	32.0	32.0	32.0	32.0
5	30.863375780663276	32.0	32.0	32.0	32.0	32.0
6	34.51524377320004	36.0	36.0	36.0	32.0	36.0
7	34.42589970434286	36.0	36.0	36.0	32.0	36.0
8	34.440433280376986	36.0	36.0	36.0	32.0	36.0
9	34.56548270946704	36.0	36.0	36.0	32.0	36.0
10	34.288267289742095	36.0	36.0	36.0	32.0	36.0
11	34.53282344656628	36.0	36.0	36.0	32.0	36.0
12	34.34763819108195	36.0	36.0	36.0	32.0	36.0
13	34.45061614744769	36.0	36.0	36.0	32.0	36.0
14	34.36965999745658	36.0	36.0	36.0	32.0	36.0
15	34.315236750314924	36.0	36.0	36.0	32.0	36.0
16	34.323048286447666	36.0	36.0	36.0	32.0	36.0
17	34.26162303303856	36.0	36.0	36.0	32.0	36.0
18	34.26574913621677	36.0	36.0	36.0	32.0	36.0
19	34.260357964679315	36.0	36.0	36.0	32.0	36.0
20	34.250990638051256	36.0	36.0	36.0	32.0	36.0
21	34.2343998628323	36.0	36.0	36.0	32.0	36.0
22	34.1929898320012	36.0	36.0	36.0	32.0	36.0
23	34.16166554996467	36.0	36.0	36.0	32.0	36.0
24	34.123334516468034	36.0	36.0	36.0	32.0	36.0
25	33.69489265425198	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	18.0
4	98.0
5	297.0
6	531.0
7	112.0
8	438.0
9	578.0
10	395.0
11	110.0
12	225.0
13	203.0
14	425.0
15	526.0
16	873.0
17	1124.0
18	1535.0
19	1962.0
20	3072.0
21	4540.0
22	6890.0
23	10455.0
24	15329.0
25	22018.0
26	31179.0
27	41260.0
28	57362.0
29	77505.0
30	101514.0
31	140851.0
32	204440.0
33	301813.0
34	703017.0
35	1430398.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.90343134463405	18.25231107989598	11.410913485570383	26.433344089899585
2	16.48939687143408	20.358297893610285	37.753989954447974	25.39831528050766
3	18.50832356048007	24.137866671812773	29.344501223834353	28.00930854387281
4	12.610823722968375	16.218594276493757	36.683656842738046	34.48692515779982
5	14.048341093347064	36.5821171688761	34.34480916632041	15.02473257145642
6	32.7492509471756	35.922728321460205	17.827504694338657	13.500516037025537
7	29.114067101423263	30.994277002692932	21.76587657711643	18.125779318767375
8	26.873376913271365	33.98399282659782	20.2738532403476	18.868777019783213
9	26.89484663524685	15.110737749248596	19.641669717031675	38.352745898472875
10	15.768518893883115	27.1697751078094	32.65785653114691	24.403849467160573
11	35.54971667974185	21.446033912283628	24.09953059196847	18.90471881600605
12	24.243628908719774	24.033297053200023	29.801458131923685	21.921615906156514
13	28.618973339773383	20.427355435376466	25.892456430681037	25.06121479416911
14	22.515383748022	20.894816592276516	25.830031914071455	30.75976774563003
15	24.208480060655564	28.605786664833992	22.627007877432185	24.55872539707826
16	24.577367816994723	26.394955396390667	25.079332605352434	23.948344181262176
17	23.217343326071685	26.631891413538337	26.27702293088933	23.873742329500644
18	23.693312136309657	25.526734318494466	27.612322527982634	23.16763101721324
19	24.90656415516226	25.56634047929999	25.98718585077696	23.53990951476079
20	24.655845184176354	25.79102397618218	25.827890919456497	23.72523992018497
21	25.097798357050955	25.154941105591817	25.349714254581713	24.397546282775515
22	24.736815091929376	25.88238387801552	25.46281511220605	23.917985917849048
23	23.459677955846438	25.6787921601921	26.265732360590615	24.59579752337085
24	24.127230706071373	25.726325752776187	26.11839404367901	24.02804949747343
25	23.917458893530537	25.61860849358426	26.33253128678422	24.131401326100978
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	252.0
1	252.0
2	752.5
3	1253.0
4	1253.0
5	1253.0
6	3381.0
7	5509.0
8	5509.0
9	5509.0
10	6334.5
11	7160.0
12	7160.0
13	7160.0
14	9851.0
15	12542.0
16	12542.0
17	12542.0
18	20538.0
19	28534.0
20	28534.0
21	28534.0
22	45113.0
23	61692.0
24	61692.0
25	61692.0
26	91659.5
27	121627.0
28	121627.0
29	121627.0
30	150998.5
31	180370.0
32	180370.0
33	180370.0
34	210384.0
35	240398.0
36	240398.0
37	240398.0
38	263269.5
39	286141.0
40	286141.0
41	286141.0
42	312098.0
43	338055.0
44	338055.0
45	338055.0
46	367955.0
47	397855.0
48	397855.0
49	397855.0
50	397417.0
51	396979.0
52	396979.0
53	396979.0
54	368685.5
55	340392.0
56	340392.0
57	340392.0
58	308262.0
59	276132.0
60	276132.0
61	276132.0
62	240344.5
63	204557.0
64	204557.0
65	204557.0
66	166873.5
67	129190.0
68	129190.0
69	129190.0
70	97622.5
71	66055.0
72	66055.0
73	66055.0
74	50019.5
75	33984.0
76	33984.0
77	33984.0
78	26505.0
79	19026.0
80	19026.0
81	19026.0
82	13297.0
83	7568.0
84	7568.0
85	7568.0
86	5466.5
87	3365.0
88	3365.0
89	3365.0
90	2222.0
91	1079.0
92	1079.0
93	1079.0
94	652.0
95	225.0
96	225.0
97	225.0
98	689.5
99	1154.0
100	1154.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007402500526716383
2	0.0029420194401052293
3	0.004017596439713593
4	0.014045770230179805
5	0.034513367840374246
6	0.045458945542271124
7	0.06595817777010111
8	0.08731154467409068
9	0.09496712214189139
10	0.11609904672243217
11	0.11745933528076039
12	0.13216943248128654
13	0.11923087386835066
14	0.12229943177899803
15	0.11366318116449559
16	0.11078443095966144
17	0.1223627010142691
18	0.1280252975710308
19	0.13017645157024751
20	0.12002173930923915
21	0.12932231689408794
22	0.1505175107098998
23	0.1386861637142078
24	0.13432058648050327
25	0.13191635554020223
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3161094.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.2291448939593
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.39346041281085	43.857334426282094
2	10.183067210455695	10.840719200205443
3	2.8093547967450845	4.4861866060345115
4	1.2289885188324738	2.616720317677847
5	0.7320985954610653	1.948449110723057
6	0.4830911736602226	1.5428718047851704
7	0.345958317008515	1.2890545768321584
8	0.25690125722882906	1.0939707395578921
9	0.19215003852755203	0.9205184017946635
>10	1.1754611752362323	11.635286715611908
>50	0.10083885501370353	3.7243398371733565
>100	0.08378888184141778	9.221766785803101
>500	0.011190417187094275	3.955699589191428
>1k	0.0036503499912307626	2.8670818883273856
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3781	0.11961048927997713	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.32692352710802E-5
2	0.0	0.0	0.0	0.0	6.32692352710802E-5
3	0.0	0.0	0.0	0.0	6.32692352710802E-5
4	0.0	0.0	0.0	0.0	9.49038529066203E-5
5	0.0	0.0	0.0	3.16346176355401E-5	9.49038529066203E-5
6	3.16346176355401E-5	0.0	0.0	3.16346176355401E-5	1.898077058132406E-4
7	3.16346176355401E-5	0.0	0.0	9.49038529066203E-5	1.898077058132406E-4
8	3.16346176355401E-5	0.0	0.0	1.581730881777005E-4	1.898077058132406E-4
9	3.16346176355401E-5	0.0	0.0	2.847115587198609E-4	1.898077058132406E-4
10	3.16346176355401E-5	0.0	0.0	2.847115587198609E-4	2.530769410843208E-4
11	3.16346176355401E-5	0.0	0.0	2.847115587198609E-4	2.530769410843208E-4
12	3.16346176355401E-5	0.0	0.0	2.847115587198609E-4	5.377884998041817E-4
13	3.16346176355401E-5	0.0	0.0	3.479807939909411E-4	6.01057735075262E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATT	120	3.6379788E-12	14.24411	2
GCGTTAT	135	0.0	14.066697	1
GTATCAA	2805	0.0	12.490776	1
CGCATCG	285	0.0	11.667756	13
TATGCCG	100	1.9344516E-6	11.398536	5
GGTATCA	1035	0.0	11.192198	1
AGAACCG	305	0.0	10.588804	5
CGTCGTA	350	0.0	10.585529	10
TACCGTC	395	0.0	10.582093	7
CCGTCGT	340	0.0	10.337892	9
ATCCCCG	195	1.4551915E-11	10.232211	12
CAACGGA	195	1.4551915E-11	10.231886	14
GTATAGA	270	0.0	10.198356	1
ACCGTCG	365	0.0	10.150507	8
CGTCTTA	370	0.0	10.015242	15
TGGCGAG	1965	0.0	9.914223	18
CGCGTAA	125	2.5727095E-6	9.8798275	10
ATACCGT	385	0.0	9.870114	6
GCATCGC	320	0.0	9.797945	14
CTTACAC	330	0.0	9.784918	3
>>END_MODULE