FastQCFastQC Report
Thu 2 Feb 2017
SRR4062345_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062345_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3161094
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29850.025.5780331
GGTATCA14100.021.2227571
CGTACCG901.0027117E-519.5557525
TCAACGC42700.017.2086054
AACGCAG43600.017.1056776
TATCAAC44400.016.9466512
CAACGCA43550.016.9232485
TAACGCA1206.4125015E-616.5001684
ATCAACG45800.016.0918663
GGCGTTA5250.015.92371742
ACGACGG5900.015.6611746
CGTTACG850.002981479515.52932344
TAAGCGG850.002981479515.52932310
CGACGGT5700.015.4385077
TAACGGC6450.015.34874936
TTAACGG6600.015.33324435
TAGGACC18300.015.2679134
CGGCGTT5500.014.79991441
GTATTAG11200.014.53771
GACGGTA6250.014.4319178
Produced by FastQC (version 0.11.5)