##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062345_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3161094
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.312881553031957	32.0	32.0	32.0	32.0	32.0
2	31.414350538136482	32.0	32.0	32.0	32.0	32.0
3	31.49251082062096	32.0	32.0	32.0	32.0	32.0
4	31.593056707582882	32.0	32.0	32.0	32.0	32.0
5	31.529730846346233	32.0	32.0	32.0	32.0	32.0
6	35.11930964406626	36.0	36.0	36.0	36.0	36.0
7	35.13168542283146	36.0	36.0	36.0	36.0	36.0
8	35.072177227251075	36.0	36.0	36.0	36.0	36.0
9	35.22050815319	36.0	36.0	36.0	36.0	36.0
10	35.084192371375224	36.0	36.0	36.0	36.0	36.0
11	35.21612011537778	36.0	36.0	36.0	36.0	36.0
12	35.13274897867637	36.0	36.0	36.0	36.0	36.0
13	35.18153145714743	36.0	36.0	36.0	36.0	36.0
14	35.139579525316236	36.0	36.0	36.0	36.0	36.0
15	35.097332126156324	36.0	36.0	36.0	36.0	36.0
16	35.11702720640386	36.0	36.0	36.0	36.0	36.0
17	35.090512335286455	36.0	36.0	36.0	36.0	36.0
18	35.10570960559857	36.0	36.0	36.0	36.0	36.0
19	35.094722270201395	36.0	36.0	36.0	36.0	36.0
20	35.08848582168072	36.0	36.0	36.0	36.0	36.0
21	35.08515406375135	36.0	36.0	36.0	36.0	36.0
22	35.06523469406478	36.0	36.0	36.0	36.0	36.0
23	35.028478115487864	36.0	36.0	36.0	36.0	36.0
24	35.00933347758719	36.0	36.0	36.0	36.0	36.0
25	34.98818731742871	36.0	36.0	36.0	36.0	36.0
26	34.944395516235836	36.0	36.0	36.0	32.0	36.0
27	34.92385104650479	36.0	36.0	36.0	32.0	36.0
28	34.91051926959464	36.0	36.0	36.0	32.0	36.0
29	34.89181372018675	36.0	36.0	36.0	32.0	36.0
30	34.87427327374637	36.0	36.0	36.0	32.0	36.0
31	34.87512867380723	36.0	36.0	36.0	32.0	36.0
32	34.84527223802898	36.0	36.0	36.0	32.0	36.0
33	34.834227960320064	36.0	36.0	36.0	32.0	36.0
34	34.825890024149864	36.0	36.0	36.0	32.0	36.0
35	34.80629585833259	36.0	36.0	36.0	32.0	36.0
36	34.77531291382034	36.0	36.0	36.0	32.0	36.0
37	34.76652608242589	36.0	36.0	36.0	32.0	36.0
38	34.73906723431825	36.0	36.0	36.0	32.0	36.0
39	34.72942879901705	36.0	36.0	36.0	32.0	36.0
40	34.73699421782459	36.0	36.0	36.0	32.0	36.0
41	34.711386627540975	36.0	36.0	36.0	32.0	36.0
42	34.705119493441195	36.0	36.0	36.0	32.0	36.0
43	34.690748519341724	36.0	36.0	36.0	32.0	36.0
44	34.658765604566014	36.0	36.0	36.0	32.0	36.0
45	34.63355977392637	36.0	36.0	36.0	32.0	36.0
46	34.62652486765658	36.0	36.0	36.0	32.0	36.0
47	34.61462677161767	36.0	36.0	36.0	32.0	36.0
48	34.58053794034597	36.0	36.0	36.0	32.0	36.0
49	34.564892091155784	36.0	36.0	36.0	32.0	36.0
50	34.0574101877388	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	16.0
21	77.0
22	241.0
23	711.0
24	1906.0
25	4353.0
26	9099.0
27	16767.0
28	28721.0
29	45323.0
30	66155.0
31	96635.0
32	144182.0
33	240153.0
34	536662.0
35	1970090.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.320684023319664	18.890594616075333	11.896308099047205	26.892413261557795
2	15.888194619540855	20.902182471953907	37.4307211391993	25.77890176930594
3	17.88640956433822	24.524117737124953	29.67442098829639	27.915051710240434
4	12.392884981373502	16.705387512147716	36.846933713961775	34.05479379251701
5	13.862194544040765	36.84414319852557	34.13033589004313	15.163326367390532
6	33.00651454772247	36.089417569127505	17.528761539967842	13.375306343182187
7	29.20998236686413	31.022139803498412	21.732792507910236	18.035085321727227
8	26.972465861502375	34.172568104586574	20.071437293544577	18.783528740366467
9	27.00580874849024	14.9249278888891	19.494991290989766	38.5742720716309
10	15.75214150544084	27.238481361199636	32.568882798170506	24.44049433518902
11	35.802130528228524	21.325781517411375	24.0005833423492	18.871504612010906
12	24.32281355758481	23.97837584076905	29.68946826636601	22.009342335280127
13	28.808760511392574	20.336978273977298	25.816536933099744	25.037724281530384
14	22.63026661023051	20.743008591329456	25.770919814469295	30.855804983970742
15	24.36608971451023	28.66551896273885	22.451973905236606	24.516417417514315
16	24.7531708958987	26.3324975467354	24.918967926926562	23.995363630439336
17	23.33489608344453	26.722900362975604	26.086348586913267	23.855854966666605
18	23.86398506339894	25.53410306684964	27.392162333673088	23.209749536078334
19	25.069959956900995	25.53603277852541	25.889391457514392	23.5046158070592
20	24.88628936690905	25.72482817657431	25.628057881227196	23.76082457528944
21	25.291339011114506	25.09909543974333	25.222913333168833	24.38665221597333
22	24.879741001058495	25.862850013318173	25.356158342649728	23.901250642973604
23	23.62685196960293	25.622743265464425	26.125195897369707	24.62520886756294
24	24.393010774118075	25.686898270029303	25.916312517122236	24.00377843873039
25	24.20073556812926	25.440812579442433	26.234778212859215	24.123673639569084
26	23.66690139552952	26.01574012035074	26.476340153124205	23.841018330995535
27	24.223322685121037	26.148478975949462	25.41778257780376	24.210415761125738
28	23.59670417899626	25.996822619004682	25.955033289108137	24.45143991289092
29	24.114752677395863	25.875187514196035	25.50088671833232	24.509173090075777
30	23.852533331814872	25.859085493819546	26.133642340278396	24.154738834087187
31	24.429896738281116	25.290326703350168	25.6820898081487	24.597686750220017
32	23.853134389549947	25.842983473443056	25.8063505862211	24.497531550785897
33	23.642479470714886	25.654599325423415	26.327309469443172	24.375611734418527
34	24.163469988554596	25.557607587752845	26.5169273675506	23.761995056141956
35	24.453591066890134	25.7141356758135	26.01621463961527	23.816058617681094
36	23.80359457833269	25.631632593020008	26.102102626495764	24.462670202151536
37	24.358592310130607	25.637769708841308	25.353849015562336	24.649788965465753
38	23.902168046885034	25.93722299937933	25.848930781558536	24.3116781721771
39	24.41689491043291	25.33926545683235	25.496362968010445	24.747476664724303
40	24.71875243191123	25.18817852300501	26.415823129587412	23.677245915496346
41	24.04490344165659	24.939530428389663	26.84383950619627	24.171726623757472
42	24.33227230825784	25.74023423536282	26.27185398472807	23.65563947165127
43	23.500155325972592	25.308674781578784	26.588484872642194	24.602685019806433
44	23.328221179123428	25.422053251184558	26.41841716823353	24.831308401458482
45	23.331954064004425	25.636725766459335	26.55558487030123	24.475735299235012
46	23.60256228154285	25.05149333030484	26.602541086315508	24.743403301836803
47	23.93634100610137	25.079268468216533	26.627277337300736	24.35711318838136
48	23.660732645090594	25.680128461855293	26.196278883196765	24.462860009857344
49	23.305926146431	25.382328201036792	26.564735678450457	24.74700997408175
50	22.814830186900544	26.121376372033346	26.43699505202789	24.62679838903822
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	35.0
1	52.5
2	70.0
3	220.5
4	371.0
5	422.5
6	474.0
7	448.0
8	422.0
9	563.0
10	704.0
11	989.0
12	1274.0
13	2138.0
14	3002.0
15	3771.0
16	4540.0
17	5514.0
18	6488.0
19	7140.5
20	7793.0
21	9760.5
22	11728.0
23	13582.5
24	15437.0
25	21253.0
26	27069.0
27	35712.5
28	44356.0
29	52664.5
30	60973.0
31	67951.5
32	74930.0
33	89406.5
34	103883.0
35	119716.0
36	135549.0
37	149245.5
38	162942.0
39	165695.5
40	168449.0
41	173684.0
42	178919.0
43	176871.5
44	174824.0
45	185175.0
46	195526.0
47	202925.0
48	210324.0
49	214380.0
50	218436.0
51	214607.5
52	210779.0
53	207164.0
54	203549.0
55	201875.0
56	200201.0
57	194376.0
58	188551.0
59	171750.0
60	154949.0
61	137155.0
62	119361.0
63	103738.5
64	88116.0
65	74379.5
66	60643.0
67	52273.5
68	43904.0
69	38099.5
70	32295.0
71	24863.0
72	17431.0
73	15278.0
74	13125.0
75	9734.5
76	6344.0
77	5561.5
78	4779.0
79	4217.5
80	3656.0
81	2884.0
82	2112.0
83	1736.5
84	1361.0
85	1111.0
86	861.0
87	617.0
88	373.0
89	236.5
90	100.0
91	69.5
92	39.0
93	28.5
94	18.0
95	17.0
96	16.0
97	11.5
98	7.0
99	6.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01581730881777005
2	0.004333942616068994
3	5.694231174397218E-4
4	1.8980770581324062E-4
5	0.0
6	4.745192645331015E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.581730881777005E-4
47	3.16346176355401E-5
48	0.0
49	3.16346176355401E-5
50	3.16346176355401E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3161094.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.28727697673175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.75563909959754	48.818881345614265
2	9.743494693692309	11.358435478651195
3	2.4837070922559685	4.343055696461899
4	1.1065966348062368	2.5800201821788162
5	0.6421476759960725	1.871451972537384
6	0.42469289572190805	1.4852515465795868
7	0.3117168732074559	1.271838940887463
8	0.22006115744475396	1.026141250864204
9	0.17127456170023095	0.8984815035200724
>10	0.9862902061630248	10.55220356271819
>50	0.08121892831517723	3.2651752628687505
>100	0.06278189452508855	7.59515971260494
>500	0.00824800669885519	3.2749207030024916
>1k	0.0021302798754151493	1.6589828415108112
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.49038529066203E-5
2	0.0	0.0	0.0	0.0	9.49038529066203E-5
3	0.0	0.0	0.0	0.0	9.49038529066203E-5
4	0.0	0.0	0.0	0.0	9.49038529066203E-5
5	0.0	0.0	0.0	3.16346176355401E-5	9.49038529066203E-5
6	0.0	0.0	0.0	3.16346176355401E-5	9.49038529066203E-5
7	0.0	0.0	0.0	3.16346176355401E-5	9.49038529066203E-5
8	0.0	0.0	0.0	6.32692352710802E-5	9.49038529066203E-5
9	0.0	0.0	0.0	1.265384705421604E-4	9.49038529066203E-5
10	0.0	0.0	0.0	1.265384705421604E-4	9.49038529066203E-5
11	0.0	0.0	0.0	1.265384705421604E-4	9.49038529066203E-5
12	3.16346176355401E-5	0.0	0.0	1.265384705421604E-4	2.214423234487807E-4
13	3.16346176355401E-5	0.0	0.0	1.265384705421604E-4	2.214423234487807E-4
14	3.16346176355401E-5	0.0	0.0	1.581730881777005E-4	2.214423234487807E-4
15	3.16346176355401E-5	0.0	0.0	2.214423234487807E-4	2.530769410843208E-4
16	3.16346176355401E-5	0.0	0.0	2.847115587198609E-4	2.530769410843208E-4
17	3.16346176355401E-5	0.0	0.0	3.479807939909411E-4	2.530769410843208E-4
18	3.16346176355401E-5	0.0	0.0	4.745192645331015E-4	2.530769410843208E-4
19	3.16346176355401E-5	0.0	0.0	5.377884998041817E-4	2.530769410843208E-4
20	3.16346176355401E-5	0.0	0.0	7.275962056174223E-4	4.112500292620213E-4
21	3.16346176355401E-5	0.0	0.0	8.541346761595827E-4	4.112500292620213E-4
22	3.16346176355401E-5	0.0	0.0	0.001202115470150524	4.428846468975614E-4
23	3.16346176355401E-5	0.0	0.0	0.001581730881777005	4.745192645331015E-4
24	3.16346176355401E-5	0.0	0.0	0.0021195193815811866	5.377884998041817E-4
25	3.16346176355401E-5	0.0	0.0	0.002752211734291989	5.377884998041817E-4
26	3.16346176355401E-5	0.0	0.0	0.0035747117928160315	6.32692352710802E-4
27	3.16346176355401E-5	0.0	0.0	0.004587019557153314	7.592308232529624E-4
28	3.16346176355401E-5	0.0	0.0	0.00945875067302649	7.592308232529624E-4
29	3.16346176355401E-5	0.0	0.0	0.019676732169305943	7.592308232529624E-4
30	3.16346176355401E-5	0.0	0.0	0.04333942616068994	7.592308232529624E-4
31	6.32692352710802E-5	0.0	0.0	0.10344519966821614	7.908654408885025E-4
32	6.32692352710802E-5	0.0	0.0	0.17658443564158485	8.857692937951228E-4
33	6.32692352710802E-5	0.0	0.0	0.2594671338466999	8.857692937951228E-4
34	6.32692352710802E-5	0.0	0.0	0.3533270443713474	9.174039114306629E-4
35	6.32692352710802E-5	0.0	0.0	0.45765801333335865	9.174039114306629E-4
36	6.32692352710802E-5	0.0	0.0	0.6069101393378369	9.174039114306629E-4
37	6.32692352710802E-5	0.0	0.0	0.8341732324315569	9.174039114306629E-4
38	6.32692352710802E-5	0.0	0.0	1.1449517160831029	9.49038529066203E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2985	0.0	25.578033	1
GGTATCA	1410	0.0	21.222757	1
CGTACCG	90	1.0027117E-5	19.555752	5
TCAACGC	4270	0.0	17.208605	4
AACGCAG	4360	0.0	17.105677	6
TATCAAC	4440	0.0	16.946651	2
CAACGCA	4355	0.0	16.923248	5
TAACGCA	120	6.4125015E-6	16.500168	4
ATCAACG	4580	0.0	16.091866	3
GGCGTTA	525	0.0	15.923717	42
ACGACGG	590	0.0	15.661174	6
CGTTACG	85	0.0029814795	15.529323	44
TAAGCGG	85	0.0029814795	15.529323	10
CGACGGT	570	0.0	15.438507	7
TAACGGC	645	0.0	15.348749	36
TTAACGG	660	0.0	15.333244	35
TAGGACC	1830	0.0	15.267913	4
CGGCGTT	550	0.0	14.799914	41
GTATTAG	1120	0.0	14.5377	1
GACGGTA	625	0.0	14.431917	8
>>END_MODULE