FastQCFastQC Report
Thu 2 Feb 2017
SRR4062344_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062344_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences970213
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCATTCCATTCCATTCCATTCC27400.28241221257600135No Hit
GTATCAACGCAGAGTACTTTTTTTT23800.24530695836893546No Hit
TATCAACGCAGAGTACTTTTTTTTT16920.1743946947732096No Hit
GAATGGAATGGAATGGAATGGAATG16200.16697364393179642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGAA755.813854E-815.2050418
TTTTCGG851.7087586E-814.53272916
ACTATAC400.005298168414.2414873
CCCTTAT400.00530361214.2392831
CGCAAGA1600.014.2378132
GCGCAAG1650.013.8077881
CGGACAT903.627065E-813.7239385
TTTCGGA852.6862836E-713.4155217
GGTATCA4950.013.2324641
GTCCTAA1153.8016879E-1013.2074491
GTTTTCG957.363269E-813.00162515
GTATCAA14650.012.8299331
GCGTTAT905.4474003E-712.657141
TATGGCG1457.2759576E-1212.45111816
AAGACGG2000.012.3515435
AGACGGA2100.012.2158126
CCGGTCC2350.012.1291769
CCGAAAG550.00305940212.09491919
ACCCGCA550.003064139512.0924217
CGGTCCA1900.012.001510
Produced by FastQC (version 0.11.5)