##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062344_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	970213
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.258091779846282	32.0	32.0	32.0	32.0	32.0
2	30.991824475656376	32.0	32.0	32.0	32.0	32.0
3	31.012273593530495	32.0	32.0	32.0	32.0	32.0
4	30.959606807989587	32.0	32.0	32.0	32.0	32.0
5	30.934190739559252	32.0	32.0	32.0	32.0	32.0
6	34.41571077691187	36.0	36.0	36.0	32.0	36.0
7	34.422405183191735	36.0	36.0	36.0	32.0	36.0
8	34.34872960885909	36.0	36.0	36.0	32.0	36.0
9	34.48706727285658	36.0	36.0	36.0	32.0	36.0
10	34.24443395419356	36.0	36.0	36.0	32.0	36.0
11	34.49558086729409	36.0	36.0	36.0	32.0	36.0
12	34.347048534703205	36.0	36.0	36.0	32.0	36.0
13	34.41604884700576	36.0	36.0	36.0	32.0	36.0
14	34.339346102350724	36.0	36.0	36.0	32.0	36.0
15	34.26894300529884	36.0	36.0	36.0	32.0	36.0
16	34.26363695394723	36.0	36.0	36.0	32.0	36.0
17	34.252027132186434	36.0	36.0	36.0	32.0	36.0
18	34.22772731348683	36.0	36.0	36.0	32.0	36.0
19	34.219582710188384	36.0	36.0	36.0	32.0	36.0
20	34.15938458874495	36.0	36.0	36.0	32.0	36.0
21	34.12715249125707	36.0	36.0	36.0	32.0	36.0
22	34.13850566834293	36.0	36.0	36.0	32.0	36.0
23	34.09113462713858	36.0	36.0	36.0	32.0	36.0
24	34.0633819583947	36.0	36.0	36.0	32.0	36.0
25	33.445358905724824	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	7.0
4	39.0
5	106.0
6	151.0
7	37.0
8	127.0
9	193.0
10	107.0
11	35.0
12	74.0
13	56.0
14	191.0
15	319.0
16	463.0
17	663.0
18	790.0
19	985.0
20	1259.0
21	1720.0
22	2276.0
23	3098.0
24	4210.0
25	5735.0
26	8173.0
27	10973.0
28	15222.0
29	21230.0
30	29656.0
31	43859.0
32	70008.0
33	112535.0
34	268821.0
35	367095.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.91098952411679	19.728738224552718	11.625931702148055	24.73434054918243
2	15.845871423461904	20.843825405054954	39.301900581028	24.008402590455145
3	18.00245732156073	25.994009247940035	29.31139335492423	26.692140075575004
4	11.963366950053246	15.58028272682008	37.66907581338339	34.787274509743284
5	14.550652971416906	36.30667488766582	33.50765150937132	15.635020631545954
6	32.35174929082804	37.60348857952161	17.0303353396034	13.014426790046949
7	27.890014232966852	31.147507168052147	23.113513067513768	17.848965531467233
8	26.164612772313795	34.26104161637669	20.91660838186961	18.65773722943991
9	26.16662178766973	14.080862667174262	20.220412618529558	39.53210292662645
10	15.645680073554374	27.035050805971444	31.82072875379613	25.498540366678053
11	36.262602288792344	23.25652429649045	22.055063101736717	18.425810312980488
12	22.745450961410445	25.03733457455098	31.119232233954392	21.097982230084185
13	27.757230396456677	21.818945465277793	26.6418928541207	23.78193128414483
14	22.134837258260923	19.814864502280656	26.777053105199066	31.273245134259355
15	24.567621866090473	27.845347878532728	22.89186960913904	24.69516064623776
16	24.960273401546136	27.970620890618747	23.91623775698466	23.15286795085046
17	22.16039553485646	27.068462163202504	27.53955812952714	23.2315841724139
18	22.687961195108105	27.104907373961506	27.960885494607563	22.246245936322822
19	23.84843230747002	24.85066154767065	27.23109790079881	24.06980824406052
20	25.015737451833076	25.023270789431862	25.79693424118651	24.16405751754855
21	26.112623058592348	25.906523459955068	24.93464568310613	23.046207798346455
22	23.83811191121094	25.742076534319878	27.594325989297747	22.825485565171437
23	22.963866638180257	26.18706283421218	27.325177549817674	23.523892977789888
24	23.18478910393555	24.999870992644517	27.090306180897212	24.725033722522724
25	24.098612583249647	25.271254784951953	26.037259926743406	24.592872705054994
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	91.0
1	91.0
2	290.5
3	490.0
4	490.0
5	490.0
6	1119.5
7	1749.0
8	1749.0
9	1749.0
10	2022.5
11	2296.0
12	2296.0
13	2296.0
14	2919.5
15	3543.0
16	3543.0
17	3543.0
18	6012.5
19	8482.0
20	8482.0
21	8482.0
22	13544.0
23	18606.0
24	18606.0
25	18606.0
26	27139.5
27	35673.0
28	35673.0
29	35673.0
30	45720.0
31	55767.0
32	55767.0
33	55767.0
34	68188.5
35	80610.0
36	80610.0
37	80610.0
38	93964.0
39	107318.0
40	107318.0
41	107318.0
42	111853.5
43	116389.0
44	116389.0
45	116389.0
46	119294.5
47	122200.0
48	122200.0
49	122200.0
50	119413.5
51	116627.0
52	116627.0
53	116627.0
54	105816.0
55	95005.0
56	95005.0
57	95005.0
58	84859.0
59	74713.0
60	74713.0
61	74713.0
62	65089.0
63	55465.0
64	55465.0
65	55465.0
66	45535.5
67	35606.0
68	35606.0
69	35606.0
70	27047.0
71	18488.0
72	18488.0
73	18488.0
74	14350.5
75	10213.0
76	10213.0
77	10213.0
78	8366.5
79	6520.0
80	6520.0
81	6520.0
82	4520.5
83	2521.0
84	2521.0
85	2521.0
86	1785.0
87	1049.0
88	1049.0
89	1049.0
90	686.5
91	324.0
92	324.0
93	324.0
94	192.5
95	61.0
96	61.0
97	61.0
98	234.0
99	407.0
100	407.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00824561204601464
2	0.003710525420706587
3	0.005256577679334331
4	0.01649122409202928
5	0.034940781044987025
6	0.04328946324157685
7	0.06524340531409083
8	0.08606357573027779
9	0.096885941540672
10	0.1168815507522575
11	0.11780918210743413
12	0.13110523153163273
13	0.12151970752814073
14	0.12296268963619329
15	0.11296488503040054
16	0.11224339397637427
17	0.12327190008791884
18	0.1301776001764561
19	0.13120830168220793
20	0.12234426873274219
21	0.13028067032703128
22	0.1489363675811394
23	0.13708330026499335
24	0.13151751213393348
25	0.13296049424198603
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	970213.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.81102532195671
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.66807850761813	49.663195901223155
2	11.896989252285714	14.46936229351587
3	3.081428727800345	5.621545211822149
4	1.1382266259403786	2.768669126887429
5	0.5399169039391173	1.6416450258597066
6	0.3062775289210599	1.1175030340058945
7	0.21418403374538977	0.9117325489754808
8	0.16040210042370776	0.7803372952448909
9	0.1111201127506846	0.608159519123451
>10	0.7288680007990452	8.605224259429116
>50	0.08115627510961164	3.51594020484593
>100	0.06826981110811232	7.692021439904856
>500	0.0044045036176640065	1.7360306942397905
>1k	6.776159411790779E-4	0.8686334449222594
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATTCCATTCCATTCCATTCCATTCC	2740	0.28241221257600135	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2380	0.24530695836893546	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1692	0.1743946947732096	No Hit
GAATGGAATGGAATGGAATGGAATG	1620	0.16697364393179642	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0307015057518298E-4
2	0.0	0.0	0.0	0.0	1.0307015057518298E-4
3	0.0	0.0	0.0	0.0	1.0307015057518298E-4
4	0.0	0.0	0.0	0.0	1.0307015057518298E-4
5	0.0	0.0	0.0	0.0	1.0307015057518298E-4
6	0.0	0.0	0.0	0.0	1.0307015057518298E-4
7	0.0	0.0	0.0	0.0	1.0307015057518298E-4
8	0.0	0.0	0.0	0.0	1.0307015057518298E-4
9	0.0	0.0	0.0	0.0	3.092104517255489E-4
10	0.0	0.0	0.0	0.0	3.092104517255489E-4
11	0.0	0.0	0.0	0.0	3.092104517255489E-4
12	0.0	0.0	0.0	0.0	3.092104517255489E-4
13	0.0	0.0	0.0	0.0	3.092104517255489E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGGAA	75	5.813854E-8	15.20504	18
TTTTCGG	85	1.7087586E-8	14.532729	16
ACTATAC	40	0.0052981684	14.241487	3
CCCTTAT	40	0.005303612	14.239283	1
CGCAAGA	160	0.0	14.237813	2
GCGCAAG	165	0.0	13.807788	1
CGGACAT	90	3.627065E-8	13.723938	5
TTTCGGA	85	2.6862836E-7	13.41552	17
GGTATCA	495	0.0	13.232464	1
GTCCTAA	115	3.8016879E-10	13.207449	1
GTTTTCG	95	7.363269E-8	13.001625	15
GTATCAA	1465	0.0	12.829933	1
GCGTTAT	90	5.4474003E-7	12.65714	1
TATGGCG	145	7.2759576E-12	12.451118	16
AAGACGG	200	0.0	12.351543	5
AGACGGA	210	0.0	12.215812	6
CCGGTCC	235	0.0	12.129176	9
CCGAAAG	55	0.003059402	12.094919	19
ACCCGCA	55	0.0030641395	12.092421	7
CGGTCCA	190	0.0	12.0015	10
>>END_MODULE