FastQCFastQC Report
Thu 2 Feb 2017
SRR4062344_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062344_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences970213
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCACCG609.750742E-625.6665465
GTATCAA20700.021.5770031
CGGCGTT2800.018.85705641
TCGCGCT708.1219996E-418.85705620
GGCGTTA2850.018.52622842
GCGGCGT2950.017.8982240
GACGGAG750.001290661617.5999187
GCGTTAT3050.017.31139443
GATATAC2550.017.25661
AACGCAG25250.017.2514046
ACCGTCG2055.456968E-1217.170658
ATAGGGG1307.3134834E-716.9229973
CGTCGTA1954.9112714E-1116.92299710
TACCGTC1854.2200554E-1016.6485717
ATGCGGC3050.016.59008635
TAGGACG1602.1276719E-816.4999244
TATACTG1602.1276719E-816.4999245
CCGTCGT2007.2759576E-1116.4999229
ATACACT3750.016.426594
TTAGGAC1906.166374E-1016.210453
Produced by FastQC (version 0.11.5)