FastQCFastQC Report
Thu 2 Feb 2017
SRR4062343_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062343_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2148925
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38310.17827518410367973No Hit
TATCAACGCAGAGTACTTTTTTTTT25800.1200600300150075No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGAC551.1344027E-515.5372013
GCGTTAT1251.03682396E-1012.9101331
CGCCAGT3500.012.76196518
GCATCGC3500.012.75869314
CGCGTAA1650.012.66820510
CGCATCG3550.012.57840613
TCGCGTA1700.012.2956119
CATCGCC3900.012.18096715
CGGCACA550.003072202712.0892774
CGAACGA1353.6925485E-1011.96636916
TAACGAA1353.7107384E-1011.96385813
CCGATAA1459.640644E-1111.7945369
CGTCGTA3150.011.76333310
CTAGACA1701.8189894E-1211.733714
TCGCCAG3850.011.60151617
TTAGGAC5100.011.5428893
CGGTTCT4450.011.52841512
TCCAATA3800.011.492022
ATCGCCA4100.011.35689216
AATAGCG3400.011.1752235
Produced by FastQC (version 0.11.5)