##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062343_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2148925
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.217997836127367	32.0	32.0	32.0	32.0	32.0
2	30.843759554195703	32.0	32.0	32.0	32.0	32.0
3	30.843415661319032	32.0	32.0	32.0	32.0	32.0
4	30.918356387496072	32.0	32.0	32.0	32.0	32.0
5	30.703408913759205	32.0	32.0	32.0	32.0	32.0
6	34.37482182951941	36.0	36.0	36.0	32.0	36.0
7	34.26227578905732	36.0	36.0	36.0	32.0	36.0
8	34.25763719068837	36.0	36.0	36.0	32.0	36.0
9	34.43146968833254	36.0	36.0	36.0	32.0	36.0
10	34.07967332503461	36.0	36.0	36.0	32.0	36.0
11	34.40701373942785	36.0	36.0	36.0	32.0	36.0
12	34.181191060646604	36.0	36.0	36.0	32.0	36.0
13	34.309242528240866	36.0	36.0	36.0	32.0	36.0
14	34.21705364310062	36.0	36.0	36.0	32.0	36.0
15	34.14394453040474	36.0	36.0	36.0	32.0	36.0
16	34.14768826273602	36.0	36.0	36.0	32.0	36.0
17	34.077721186174486	36.0	36.0	36.0	32.0	36.0
18	34.08089672743348	36.0	36.0	36.0	32.0	36.0
19	34.075688076596435	36.0	36.0	36.0	32.0	36.0
20	34.06370301429784	36.0	36.0	36.0	32.0	36.0
21	34.05716253475575	36.0	36.0	36.0	32.0	36.0
22	34.00045604197447	36.0	36.0	36.0	32.0	36.0
23	33.972529985923195	36.0	36.0	36.0	32.0	36.0
24	33.94167735030306	36.0	36.0	36.0	32.0	36.0
25	33.48017497120653	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	13.0
4	76.0
5	220.0
6	343.0
7	86.0
8	314.0
9	407.0
10	246.0
11	97.0
12	163.0
13	104.0
14	306.0
15	351.0
16	666.0
17	849.0
18	1174.0
19	1684.0
20	2605.0
21	3823.0
22	5872.0
23	8866.0
24	12533.0
25	17708.0
26	24720.0
27	32413.0
28	43704.0
29	58623.0
30	75206.0
31	103575.0
32	148652.0
33	214895.0
34	478450.0
35	910181.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.19793941768864	17.539196719152347	11.430935634493764	25.83192822866525
2	16.609084943899706	19.881377307651142	37.59408608405504	25.915451664394112
3	18.843981638328685	24.085220567156735	28.280398953101788	28.790398841412788
4	12.41144291156997	15.413424654218952	36.195472988633604	35.979659445577475
5	14.598462334837855	36.69327508900508	33.584986816820845	15.123275759336224
6	34.129834408474	35.51046213667446	17.06359034906068	13.29611310579086
7	30.323359952092755	30.30221892326971	21.003099908125	18.37132121651254
8	28.034959921379766	33.17396589707642	19.54295002864422	19.24812415289959
9	27.332153946335097	14.38749663464808	18.841452565073563	39.43889685394326
10	15.827179923694459	27.0192804505838	31.690234353706792	25.463305272014953
11	37.30317762408481	21.243981243142635	22.381599084985897	19.071242047786658
12	24.665425647166817	23.79313960071479	29.01748998758204	22.523944764536353
13	29.37347270139376	19.81405685452779	25.359225291249583	25.453245152828867
14	23.123773173137447	20.07078263924383	25.367226873920522	31.438217313698203
15	24.92337494077543	27.993595128048522	22.248777190307344	24.8342527408687
16	25.412964990333325	25.954019240176095	24.20805478558617	24.424960983904402
17	23.629521398280012	26.303805536426978	25.51290736497423	24.553765700318785
18	24.509967919073127	25.072374291458832	26.674650010367472	23.74300777910057
19	25.374542655366707	25.165184587369367	25.437028559925896	24.023244197338034
20	25.458038954044216	24.71476813379527	25.234540544670796	24.592652367489716
21	26.521058443646123	24.41824273007264	24.590922433917164	24.46977639236407
22	25.5514740150552	24.90469328481468	25.074520996372286	24.469311703757835
23	24.121302948414513	24.69317541600786	25.759191922580992	25.426329712996633
24	24.483102151279173	25.291276341147924	25.572813959643483	24.652807547929427
25	24.488145095605272	25.069101576988384	25.587293510695602	24.855459816710745
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	250.0
1	250.0
2	601.5
3	953.0
4	953.0
5	953.0
6	2339.5
7	3726.0
8	3726.0
9	3726.0
10	4236.5
11	4747.0
12	4747.0
13	4747.0
14	5812.5
15	6878.0
16	6878.0
17	6878.0
18	11269.0
19	15660.0
20	15660.0
21	15660.0
22	25119.5
23	34579.0
24	34579.0
25	34579.0
26	50883.0
27	67187.0
28	67187.0
29	67187.0
30	84179.5
31	101172.0
32	101172.0
33	101172.0
34	122793.0
35	144414.0
36	144414.0
37	144414.0
38	163142.5
39	181871.0
40	181871.0
41	181871.0
42	204208.0
43	226545.0
44	226545.0
45	226545.0
46	251989.5
47	277434.0
48	277434.0
49	277434.0
50	279260.0
51	281086.0
52	281086.0
53	281086.0
54	260776.0
55	240466.0
56	240466.0
57	240466.0
58	219980.0
59	199494.0
60	199494.0
61	199494.0
62	176338.0
63	153182.0
64	153182.0
65	153182.0
66	126382.5
67	99583.0
68	99583.0
69	99583.0
70	75330.5
71	51078.0
72	51078.0
73	51078.0
74	39762.0
75	28446.0
76	28446.0
77	28446.0
78	23011.0
79	17576.0
80	17576.0
81	17576.0
82	12314.5
83	7053.0
84	7053.0
85	7053.0
86	5146.0
87	3239.0
88	3239.0
89	3239.0
90	2193.5
91	1148.0
92	1148.0
93	1148.0
94	696.5
95	245.0
96	245.0
97	245.0
98	579.0
99	913.0
100	913.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009865397815186663
2	0.004420815058692137
3	0.004700024430820062
4	0.014472352455297417
5	0.03411007829496143
6	0.04602301150575288
7	0.06710331910141117
8	0.08818362669706947
9	0.09525693079097688
10	0.11885012273578649
11	0.11680258734018173
12	0.13215910280721757
13	0.11959468106146096
14	0.12266598415486814
15	0.11326593529322801
16	0.11052037646730342
17	0.12243330967809486
18	0.12983235803948487
19	0.1322056377025722
20	0.12103726281745525
21	0.12820363670207197
22	0.14988889793734075
23	0.13923240690112498
24	0.13276405644682807
25	0.1307165210512233
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2148925.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.33476521244372
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.32028634929547	39.17883980687557
2	13.939937813132508	14.312068694264472
3	4.521512433461288	6.96332337530641
4	1.8251459046976906	3.7477374598443656
5	0.9429015788537496	2.4201815584449866
6	0.5497099290622096	1.6931538086014573
7	0.37451009281977354	1.3457771379215506
8	0.24529717956672217	1.0073818496265865
9	0.18947890588281274	0.8754169631584423
>10	0.9105394971199227	8.48280199619848
>50	0.08203270461152491	2.9569151846872135
>100	0.08164893034610868	8.988174423390095
>500	0.01199906904709658	4.176272479798093
>1k	0.004999612102956905	3.851955261882243
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3831	0.17827518410367973	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2580	0.1200600300150075	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3960468606396222E-4
2	0.0	0.0	0.0	0.0	1.3960468606396222E-4
3	0.0	0.0	0.0	0.0	1.3960468606396222E-4
4	0.0	0.0	0.0	0.0	1.3960468606396222E-4
5	0.0	0.0	0.0	0.0	1.8613958141861628E-4
6	0.0	0.0	0.0	0.0	2.7920937212792444E-4
7	0.0	0.0	0.0	0.0	2.7920937212792444E-4
8	0.0	0.0	0.0	0.0	2.7920937212792444E-4
9	0.0	0.0	0.0	0.0	2.7920937212792444E-4
10	4.653489535465407E-5	0.0	0.0	4.653489535465407E-5	2.7920937212792444E-4
11	4.653489535465407E-5	0.0	0.0	4.653489535465407E-5	2.7920937212792444E-4
12	4.653489535465407E-5	0.0	0.0	4.653489535465407E-5	4.653489535465407E-4
13	4.653489535465407E-5	0.0	0.0	4.653489535465407E-5	5.584187442558489E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTAGAC	55	1.1344027E-5	15.537201	3
GCGTTAT	125	1.03682396E-10	12.910133	1
CGCCAGT	350	0.0	12.761965	18
GCATCGC	350	0.0	12.758693	14
CGCGTAA	165	0.0	12.668205	10
CGCATCG	355	0.0	12.578406	13
TCGCGTA	170	0.0	12.295611	9
CATCGCC	390	0.0	12.180967	15
CGGCACA	55	0.0030722027	12.089277	4
CGAACGA	135	3.6925485E-10	11.966369	16
TAACGAA	135	3.7107384E-10	11.963858	13
CCGATAA	145	9.640644E-11	11.794536	9
CGTCGTA	315	0.0	11.763333	10
CTAGACA	170	1.8189894E-12	11.73371	4
TCGCCAG	385	0.0	11.601516	17
TTAGGAC	510	0.0	11.542889	3
CGGTTCT	445	0.0	11.528415	12
TCCAATA	380	0.0	11.49202	2
ATCGCCA	410	0.0	11.356892	16
AATAGCG	340	0.0	11.175223	5
>>END_MODULE