##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062343_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2148925
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.259032772200054	32.0	32.0	32.0	32.0	32.0
2	31.392076968716918	32.0	32.0	32.0	32.0	32.0
3	31.495392812685413	32.0	32.0	32.0	32.0	32.0
4	31.603176006607956	32.0	32.0	32.0	32.0	32.0
5	31.532582105005993	32.0	32.0	32.0	32.0	32.0
6	35.13996347010715	36.0	36.0	36.0	36.0	36.0
7	35.14605209581535	36.0	36.0	36.0	36.0	36.0
8	35.084888025408056	36.0	36.0	36.0	36.0	36.0
9	35.22984282839094	36.0	36.0	36.0	36.0	36.0
10	35.095457030841004	36.0	36.0	36.0	36.0	36.0
11	35.228409553614014	36.0	36.0	36.0	36.0	36.0
12	35.13639005549286	36.0	36.0	36.0	36.0	36.0
13	35.18891119978594	36.0	36.0	36.0	36.0	36.0
14	35.148607326919276	36.0	36.0	36.0	36.0	36.0
15	35.10460858336145	36.0	36.0	36.0	36.0	36.0
16	35.11796409832824	36.0	36.0	36.0	36.0	36.0
17	35.09409728119874	36.0	36.0	36.0	36.0	36.0
18	35.10939469734868	36.0	36.0	36.0	36.0	36.0
19	35.09538536710215	36.0	36.0	36.0	36.0	36.0
20	35.08695929360029	36.0	36.0	36.0	36.0	36.0
21	35.08367020719662	36.0	36.0	36.0	36.0	36.0
22	35.06442011703526	36.0	36.0	36.0	36.0	36.0
23	35.0296739067208	36.0	36.0	36.0	36.0	36.0
24	35.01251974824621	36.0	36.0	36.0	36.0	36.0
25	34.981617785637006	36.0	36.0	36.0	36.0	36.0
26	34.94250846353409	36.0	36.0	36.0	32.0	36.0
27	34.91984410810056	36.0	36.0	36.0	32.0	36.0
28	34.90968554044464	36.0	36.0	36.0	32.0	36.0
29	34.881491443396115	36.0	36.0	36.0	32.0	36.0
30	34.87015414684086	36.0	36.0	36.0	32.0	36.0
31	34.86866363414265	36.0	36.0	36.0	32.0	36.0
32	34.83298300312947	36.0	36.0	36.0	32.0	36.0
33	34.823462894237814	36.0	36.0	36.0	32.0	36.0
34	34.805962981490744	36.0	36.0	36.0	32.0	36.0
35	34.7845369196226	36.0	36.0	36.0	32.0	36.0
36	34.75268099165862	36.0	36.0	36.0	32.0	36.0
37	34.751539025326615	36.0	36.0	36.0	32.0	36.0
38	34.71675605244483	36.0	36.0	36.0	32.0	36.0
39	34.70596926370162	36.0	36.0	36.0	32.0	36.0
40	34.70054236420536	36.0	36.0	36.0	32.0	36.0
41	34.67346370859848	36.0	36.0	36.0	32.0	36.0
42	34.65255232267297	36.0	36.0	36.0	32.0	36.0
43	34.64506718475517	36.0	36.0	36.0	32.0	36.0
44	34.611043661365564	36.0	36.0	36.0	32.0	36.0
45	34.58021243179729	36.0	36.0	36.0	32.0	36.0
46	34.56301964935956	36.0	36.0	36.0	32.0	36.0
47	34.550397524343566	36.0	36.0	36.0	32.0	36.0
48	34.50518142792326	36.0	36.0	36.0	32.0	36.0
49	34.490092488104516	36.0	36.0	36.0	32.0	36.0
50	33.984562048466096	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	5.0
20	12.0
21	51.0
22	171.0
23	538.0
24	1348.0
25	3022.0
26	6245.0
27	11742.0
28	20006.0
29	30724.0
30	45501.0
31	66164.0
32	99266.0
33	166451.0
34	372133.0
35	1325543.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.54850120032431	18.173161325634627	12.001753711510734	26.276583762530336
2	16.012950230868533	20.330671124261347	37.290503443249904	26.36587520162022
3	18.279541871343504	24.35372066660586	28.669989892578396	28.696747569472237
4	12.18421346536865	15.889051244345998	36.39386296372131	35.53287232656404
5	14.334243556310042	36.93104083718177	33.53743547933757	15.19728012717062
6	34.31429212282646	35.760042961155335	16.753035713787124	13.172629202231075
7	30.200197959629076	30.43124393009894	21.00531289394734	18.363245216324643
8	28.003689288220524	33.35939288685872	19.367739389573575	19.26917843534718
9	27.44516444268646	14.073501867212675	18.754726200309456	39.72660748979141
10	15.769326523726978	27.13719650522936	31.712879695661783	25.380597275381877
11	37.52150493851577	21.084914550298407	22.400362972183764	18.993217539002057
12	24.599648661540073	23.765510662307896	29.03558756122247	22.599253114929557
13	29.462126412043233	19.653082355131055	25.449375850716056	25.43541538210966
14	23.212955314866733	19.905766836906825	25.377991321242018	31.503286526984418
15	24.96155054271322	27.95951464104145	22.1497260258036	24.92920879044173
16	25.499959281966568	25.922914945845015	24.1260164966204	24.451109275568015
17	23.757646264992964	26.23153437183708	25.380085391532976	24.63073397163698
18	24.560652419232873	24.945449468920508	26.679898088579172	23.814000023267447
19	25.475668066591435	25.099805716811897	25.40023500122154	24.02429121537513
20	25.547052596065473	24.58410600649162	25.237874751329155	24.630966646113755
21	26.62978000628221	24.345381993322242	24.606256616680433	24.418581383715114
22	25.563060600067477	24.86759658899217	25.118791954116592	24.45055085682376
23	24.15319287550752	24.518166059774074	25.86046511627907	25.468175948439338
24	24.675547075863513	25.14685249601545	25.557755621997046	24.619844806123993
25	24.685459008574053	24.823341903509892	25.628116383773282	24.86308270414277
26	24.257617180683365	25.541049594564726	26.040741300882996	24.16059192386891
27	25.076352129553147	25.10711169538257	25.1303791430599	24.686157032004374
28	24.184417790290492	25.10487802040555	25.75366753144014	24.957036657863814
29	24.30982933327129	25.040613329920774	25.66929976616215	24.98025757064579
30	24.097816350035483	25.233081657107626	26.077224658841047	24.591877334015848
31	24.960107960957224	24.98453878101842	25.004734925602335	25.050618332422026
32	24.43803296997336	25.17844968996126	25.121118698884327	25.262398641181054
33	24.068034017008504	24.87336691601615	25.74538432006701	25.313214746908336
34	24.82338843840525	24.97402189466826	25.939388298800566	24.263201368125923
35	25.26612143280943	24.938608839303374	25.693497911746572	24.10177181614063
36	24.101027257814955	25.392649813278734	25.51922472864339	24.98709820026292
37	25.13721977267704	25.095012622590364	25.167839733820397	24.5999278709122
38	24.28679456007073	25.08063333992578	25.686610747234084	24.94596135276941
39	25.11707016298847	24.78169317216748	25.07425805926219	25.026978605581863
40	25.330944542038463	24.875321381621042	25.634398594646164	24.159335481694335
41	24.25277755156648	25.25146294077271	26.084484102516374	24.411275405144433
42	25.43043614830671	25.444257012227045	25.47324825203299	23.652058587433253
43	24.412113033260816	24.638691438742626	25.8622799771979	25.086915550798654
44	24.10256290936166	25.25718673290133	25.53420896494759	25.106041392789418
45	24.179950440336448	25.346766406459043	25.591633025815234	24.88165012738928
46	24.177123062518728	24.90195092986071	25.653769049068277	25.267156958552278
47	24.527624057435258	24.82568020088193	25.977745141289315	24.6689506003935
48	24.990541782519166	25.40875088707144	25.083146224274927	24.517561106134465
49	24.246634945379668	25.47911164884768	25.243179729399586	25.031073676373072
50	24.109414758269722	25.825855172169888	25.157520694077597	24.9072093754828
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	38.0
1	49.5
2	61.0
3	175.5
4	290.0
5	335.0
6	380.0
7	320.0
8	260.0
9	360.5
10	461.0
11	707.5
12	954.0
13	1783.5
14	2613.0
15	3425.5
16	4238.0
17	4702.0
18	5166.0
19	5755.5
20	6345.0
21	7560.5
22	8776.0
23	9929.0
24	11082.0
25	13589.0
26	16096.0
27	20098.5
28	24101.0
29	27877.0
30	31653.0
31	35765.5
32	39878.0
33	46226.0
34	52574.0
35	60866.0
36	69158.0
37	79653.0
38	90148.0
39	95308.0
40	100468.0
41	107390.5
42	114313.0
43	116482.5
44	118652.0
45	128536.5
46	138421.0
47	146892.0
48	155363.0
49	158852.0
50	162341.0
51	155385.5
52	148430.0
53	145615.0
54	142800.0
55	144610.0
56	146420.0
57	141899.0
58	137378.0
59	125530.0
60	113682.0
61	100309.5
62	86937.0
63	75924.0
64	64911.0
65	55919.0
66	46927.0
67	40695.0
68	34463.0
69	31527.0
70	28591.0
71	21787.5
72	14984.0
73	13627.5
74	12271.0
75	8782.5
76	5294.0
77	4828.0
78	4362.0
79	3779.0
80	3196.0
81	2448.0
82	1700.0
83	1522.5
84	1345.0
85	1096.5
86	848.0
87	613.0
88	378.0
89	236.5
90	95.0
91	64.0
92	33.0
93	28.0
94	23.0
95	19.0
96	15.0
97	12.0
98	9.0
99	6.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01577532952522773
2	0.0038623963144362883
3	4.188140581918867E-4
4	2.3267447677327035E-4
5	4.653489535465407E-5
6	3.257442674825785E-4
7	9.306979070930814E-5
8	4.653489535465407E-5
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	4.653489535465407E-5
47	4.653489535465407E-5
48	0.0
49	0.0
50	4.653489535465407E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2148925.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.19728657124067
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.71017665575455	41.57483427142425
2	14.082921580132332	15.265122732774831
3	4.338214555065199	7.053583723451876
4	1.716759640137164	3.721748567618155
5	0.9131430824940412	2.4744938661237805
6	0.5268801260760572	1.7133283908981292
7	0.33110391894323754	1.256145378587924
8	0.24034855427424928	1.0420991578387904
9	0.16554928907248467	0.8075090035373952
>10	0.8217888372505014	7.920285364762836
>50	0.07257407364120123	2.7956416481422197
>100	0.06706180405978808	7.91139138352967
>500	0.00984922226498664	3.640149052029079
>1k	0.0036286608344687525	2.823667459281053
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.306979070930814E-5
2	0.0	0.0	0.0	0.0	9.306979070930814E-5
3	0.0	0.0	0.0	0.0	9.306979070930814E-5
4	0.0	0.0	0.0	0.0	9.306979070930814E-5
5	0.0	0.0	0.0	0.0	9.306979070930814E-5
6	0.0	0.0	0.0	0.0	2.7920937212792444E-4
7	0.0	0.0	0.0	0.0	2.7920937212792444E-4
8	0.0	0.0	0.0	0.0	3.7227916283723257E-4
9	0.0	0.0	0.0	0.0	3.7227916283723257E-4
10	0.0	0.0	0.0	4.653489535465407E-5	3.7227916283723257E-4
11	0.0	0.0	0.0	4.653489535465407E-5	3.7227916283723257E-4
12	0.0	0.0	0.0	4.653489535465407E-5	6.98023430319811E-4
13	0.0	0.0	0.0	4.653489535465407E-5	7.445583256744651E-4
14	0.0	0.0	0.0	4.653489535465407E-5	7.445583256744651E-4
15	0.0	0.0	0.0	4.653489535465407E-5	7.910932210291193E-4
16	0.0	0.0	0.0	9.306979070930814E-5	7.910932210291193E-4
17	0.0	0.0	0.0	1.3960468606396222E-4	7.910932210291193E-4
18	0.0	0.0	0.0	3.257442674825785E-4	7.910932210291193E-4
19	0.0	0.0	0.0	3.7227916283723257E-4	7.910932210291193E-4
20	0.0	0.0	0.0	4.1881405819188663E-4	8.841630117384274E-4
21	0.0	0.0	0.0	7.445583256744651E-4	8.841630117384274E-4
22	0.0	0.0	0.0	9.306979070930814E-4	9.306979070930814E-4
23	0.0	0.0	0.0	0.0018148609188315089	9.306979070930814E-4
24	0.0	0.0	0.0	0.0027455588259245904	9.772328024477354E-4
25	0.0	0.0	0.0	0.003815861419081634	9.772328024477354E-4
26	0.0	0.0	0.0	0.004886164012238677	0.0010703025931570436
27	0.0	0.0	0.0	0.005956466605395721	0.0011168374885116978
28	0.0	0.0	0.0	0.009632723338413392	0.0011168374885116978
29	0.0	0.0	0.0	0.017404050862640623	0.0011168374885116978
30	0.0	0.0	0.0	0.03406354339960678	0.0011168374885116978
31	0.0	0.0	0.0	0.0663587607757367	0.0012099072792210058
32	0.0	0.0	0.0	0.10488965412939028	0.0012099072792210058
33	0.0	0.0	0.0	0.1427690589480787	0.0012099072792210058
34	0.0	0.0	0.0	0.1881405819188664	0.00125644217457566
35	4.653489535465407E-5	0.0	0.0	0.23816559442511953	0.00125644217457566
36	4.653489535465407E-5	0.0	0.0	0.3183452191211885	0.00125644217457566
37	4.653489535465407E-5	0.0	0.0	0.4279814325767535	0.00125644217457566
38	9.306979070930814E-5	0.0	0.0	0.5765208185488093	0.0013495119652849681
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAATC	40	7.0340117E-4	27.49994	15
ATGCGCG	50	0.0025811172	21.999952	9
GTATCAA	3645	0.0	20.160486	1
TAGGACG	805	0.0	19.130394	4
TCGTACC	60	0.00741402	18.333294	15
GTCCTAC	995	0.0	18.13188	1
CGCAATA	620	0.0	18.096737	36
AGGACGT	875	0.0	17.599962	5
CTAGCGG	670	0.0	17.07459	29
TGTAGGA	1215	0.0	16.658789	2
GGACCGT	120	6.4113246E-6	16.499966	6
GGACGTG	930	0.0	16.322546	6
CGAATGC	690	0.0	16.260834	43
TAGCGGC	705	0.0	16.226917	30
GTAGGAC	1235	0.0	16.210493	3
AATACGA	685	0.0	16.058361	39
ATACGAA	700	0.0	16.028538	40
TCTAGCG	715	0.0	15.999968	28
AACGCAG	4610	0.0	15.939229	6
GTATTAG	640	0.0	15.81357	1
>>END_MODULE