FastQCFastQC Report
Thu 2 Feb 2017
SRR4062342_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062342_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2362288
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG106790.45206172998381233No Hit
TCCATGTACTCTGCGTTGATACCAC87880.3720122186625847No Hit
GTATCAACGCAGAGTACTTTTTTTT67570.2860362496020807No Hit
TATCAACGCAGAGTACTTTTTTTTT47060.19921364372168No Hit
GTACTTTTTTTTTTTTTTTTTTTTT46470.1967160651029849No Hit
GAGTACATGGAAGCAGTGGTATCAA39920.16898870925136986No Hit
CATGTACTCTGCGTTGATACCACTG35930.15209830469443184No Hit
CCCATGTACTCTGCGTTGATACCAC31400.1329219807237729No Hit
GTACATGGGAAGCAGTGGTATCAAC30600.1295354334441863No Hit
GAGTACTTTTTTTTTTTTTTTTTTT27360.11581991696186071No Hit
GCTTCCATGTACTCTGCGTTGATAC27060.11454996173201573No Hit
GTACATGGGGTGGTATCAACGCAAA25650.10858117215174443No Hit
GTCTTGCGCCGGTCCAAGAATTTCA25350.10731121692189945No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24620.1042209925292767No Hit
GGTATCAACGCAGAGTACTTTTTTT24390.10324736018639558No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG24240.10261238257147308No Hit
CCATAGGGTCTTCTCGTCTTATTAT24170.10231605968450926No Hit
GCGTTGATACCACTGCTTCCATGTA23850.10096144077267463No Hit
CATGGAAGCAGTGGTATCAACGCAG23820.10083444524969012No Hit
GAATAGGACCGCGGTTCTATTTTGT23820.10083444524969012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGAC2300.013.21809915
CGCATCG3750.012.66707713
AATCGTA2700.012.66707613
CGGTCCA5300.012.18984910
AGGATCG1103.8187864E-812.0905325
TCCAACG2550.011.92498718
CGCCGGT5200.011.8763917
CAACGGA2700.011.61124114
GCATCGC4050.011.49395514
CGCCAGT4150.011.44906518
CCCCTAG1001.9448453E-611.3931261
GATATAC6250.011.3931261
GTCTAAA1105.010679E-711.2205021
ACTGTTC6700.011.2028058
GTCTTAA855.358162E-511.169731
CGAACGA1900.011.00175516
TCTAGAT3650.010.9258392
TCGCCAG4350.010.92220817
CGGACAT2350.010.9145535
CGCGCTA700.00148991410.85887516
Produced by FastQC (version 0.11.5)