##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062342_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2362288
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15079956381271	32.0	32.0	32.0	32.0	32.0
2	30.819981729577428	32.0	32.0	32.0	32.0	32.0
3	30.83181263249866	32.0	32.0	32.0	32.0	32.0
4	30.884778655269805	32.0	32.0	32.0	32.0	32.0
5	30.686240627730403	32.0	32.0	32.0	32.0	32.0
6	34.3446095480314	36.0	36.0	36.0	32.0	36.0
7	34.1994782177279	36.0	36.0	36.0	32.0	36.0
8	34.18937995705858	36.0	36.0	36.0	32.0	36.0
9	34.35247268749619	36.0	36.0	36.0	32.0	36.0
10	34.02936856132698	36.0	36.0	36.0	32.0	36.0
11	34.371525402491145	36.0	36.0	36.0	32.0	36.0
12	34.132773395961884	36.0	36.0	36.0	32.0	36.0
13	34.27027652851812	36.0	36.0	36.0	32.0	36.0
14	34.16794099618675	36.0	36.0	36.0	32.0	36.0
15	34.12274117296452	36.0	36.0	36.0	32.0	36.0
16	34.10976096056027	36.0	36.0	36.0	32.0	36.0
17	34.02286512059495	36.0	36.0	36.0	32.0	36.0
18	34.05265615369506	36.0	36.0	36.0	32.0	36.0
19	34.024941920714156	36.0	36.0	36.0	32.0	36.0
20	34.002153420751405	36.0	36.0	36.0	32.0	36.0
21	33.96289741132326	36.0	36.0	36.0	32.0	36.0
22	33.91552511802117	36.0	36.0	36.0	32.0	36.0
23	33.878885216366506	36.0	36.0	36.0	32.0	36.0
24	33.84938500301403	36.0	36.0	36.0	32.0	36.0
25	33.38996388247326	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3.0
3	15.0
4	81.0
5	256.0
6	411.0
7	101.0
8	379.0
9	434.0
10	274.0
11	85.0
12	179.0
13	128.0
14	508.0
15	576.0
16	889.0
17	1208.0
18	1657.0
19	2464.0
20	3562.0
21	5172.0
22	7570.0
23	10900.0
24	14804.0
25	20389.0
26	27989.0
27	35834.0
28	47979.0
29	64011.0
30	83226.0
31	113924.0
32	164837.0
33	238997.0
34	530810.0
35	982636.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.34071165120556	17.55140436975245	11.860980433994792	25.246903545047196
2	16.32969871640463	20.015214675515505	37.501889134376405	26.15319747370346
3	19.04221037623065	24.40567309353103	28.11755980099637	28.43455672924195
4	12.358763729878053	15.9668167005446	35.63246032555841	36.04195924401893
5	14.604885431660236	36.78793645711982	33.141457990056026	15.465720121163923
6	33.783305896498405	35.01514708105817	17.45550214066605	13.746044881777381
7	30.364590967280602	30.659979717175755	20.567156566627894	18.40827274891575
8	28.51985758417647	32.334984795335515	19.570947544860857	19.574210075627157
9	27.295470204332762	15.181480812924212	18.901648916471114	38.62140006627192
10	16.45185544341953	26.41093040879261	30.939724578246665	26.197489569541194
11	36.67457828261422	21.5615410697635	22.25993896753412	19.503941680088158
12	25.00152600568013	24.031537450722734	28.27921665041753	22.687719893179604
13	29.33455740084015	19.684556786263318	25.54901843603336	25.431867376863178
14	23.833917760816924	20.46277501399786	24.845790405499287	30.85751681968593
15	25.64890193650265	27.050602166587627	22.99959780688204	24.300898090027687
16	25.824673912905283	25.88633771676098	24.299310594434832	23.989677775898905
17	23.618961153785673	25.900202981440913	25.81089792902982	24.669937935743594
18	24.67090747938125	24.684641303458594	27.195599750586975	23.44885146657319
19	25.11023206169774	25.907014243482934	25.467103327774748	23.51565036704458
20	25.04825440689676	24.49047839041778	26.075138702280693	24.38612850040477
21	26.46226531060342	24.61556590377512	24.2511599359439	24.67100884967756
22	25.095169554221954	25.09856127460852	24.885010079769025	24.921259091400504
23	24.031437582663546	24.79380744056703	25.452784277817344	25.721970698952077
24	24.19226712876928	26.19996337542898	25.103642449234275	24.504127046567465
25	24.799056598175085	24.878153821633642	25.329932067947315	24.992857512243962
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	528.0
1	528.0
2	1213.5
3	1899.0
4	1899.0
5	1899.0
6	4703.5
7	7508.0
8	7508.0
9	7508.0
10	7117.0
11	6726.0
12	6726.0
13	6726.0
14	6812.0
15	6898.0
16	6898.0
17	6898.0
18	11547.0
19	16196.0
20	16196.0
21	16196.0
22	26692.5
23	37189.0
24	37189.0
25	37189.0
26	57550.0
27	77911.0
28	77911.0
29	77911.0
30	96753.0
31	115595.0
32	115595.0
33	115595.0
34	136717.5
35	157840.0
36	157840.0
37	157840.0
38	171261.5
39	184683.0
40	184683.0
41	184683.0
42	208776.0
43	232869.0
44	232869.0
45	232869.0
46	283105.0
47	333341.0
48	333341.0
49	333341.0
50	320930.5
51	308520.0
52	308520.0
53	308520.0
54	279304.0
55	250088.0
56	250088.0
57	250088.0
58	231281.5
59	212475.0
60	212475.0
61	212475.0
62	189899.5
63	167324.0
64	167324.0
65	167324.0
66	139656.0
67	111988.0
68	111988.0
69	111988.0
70	86066.0
71	60144.0
72	60144.0
73	60144.0
74	47318.5
75	34493.0
76	34493.0
77	34493.0
78	28553.0
79	22613.0
80	22613.0
81	22613.0
82	15708.0
83	8803.0
84	8803.0
85	8803.0
86	6430.0
87	4057.0
88	4057.0
89	4057.0
90	2730.0
91	1403.0
92	1403.0
93	1403.0
94	852.0
95	301.0
96	301.0
97	301.0
98	598.5
99	896.0
100	896.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009820987110801054
2	0.004021524894509052
3	0.005079820919379855
4	0.01595910405505171
5	0.036363051414560796
6	0.04686134798127917
7	0.07069417446136965
8	0.0913097810258529
9	0.09795588006204153
10	0.12073041051726123
11	0.12225435679307518
12	0.13495390909152483
13	0.12449794436580129
14	0.12695319114350156
15	0.11535426671091754
16	0.11510027566494856
17	0.12525991750370827
18	0.13330296729272637
19	0.13436126331759718
20	0.12352431202292016
21	0.1314826981299486
22	0.15252162310438017
23	0.14088036683080132
24	0.13613920063938012
25	0.13402260858963852
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2362288.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.62373285958525
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.03959427394007	25.284876574229326
2	15.63412794187309	11.451602504714748
3	6.117611910547168	6.721493530514892
4	2.9455835200428426	4.315130558145834
5	1.6299565312441084	2.984754628651022
6	1.0142913756003022	2.2288281829060024
7	0.6502986874013862	1.667145578442911
8	0.4656071352640365	1.3641817071541478
9	0.3520210578404137	1.1603092664966328
>10	1.8077778836398262	11.952699148727367
>50	0.15358424740866208	3.928818510771214
>100	0.15305288064889677	12.26529070623455
>500	0.023633273422362266	6.068402086298438
>1k	0.012511732988309437	7.49382551328273
>5k	2.3169875904276735E-4	0.6595489692960714
>10k+	1.1584937952138368E-4	0.4530925341339818
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	10679	0.45206172998381233	No Hit
TCCATGTACTCTGCGTTGATACCAC	8788	0.3720122186625847	No Hit
GTATCAACGCAGAGTACTTTTTTTT	6757	0.2860362496020807	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4706	0.19921364372168	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4647	0.1967160651029849	No Hit
GAGTACATGGAAGCAGTGGTATCAA	3992	0.16898870925136986	No Hit
CATGTACTCTGCGTTGATACCACTG	3593	0.15209830469443184	No Hit
CCCATGTACTCTGCGTTGATACCAC	3140	0.1329219807237729	No Hit
GTACATGGGAAGCAGTGGTATCAAC	3060	0.1295354334441863	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2736	0.11581991696186071	No Hit
GCTTCCATGTACTCTGCGTTGATAC	2706	0.11454996173201573	No Hit
GTACATGGGGTGGTATCAACGCAAA	2565	0.10858117215174443	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2535	0.10731121692189945	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2462	0.1042209925292767	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2439	0.10324736018639558	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2424	0.10261238257147308	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	2417	0.10231605968450926	No Hit
GCGTTGATACCACTGCTTCCATGTA	2385	0.10096144077267463	No Hit
CATGGAAGCAGTGGTATCAACGCAG	2382	0.10083444524969012	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2382	0.10083444524969012	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.233184099483213E-5
2	0.0	0.0	0.0	0.0	4.233184099483213E-5
3	0.0	0.0	0.0	0.0	4.233184099483213E-5
4	0.0	0.0	0.0	0.0	4.233184099483213E-5
5	0.0	0.0	0.0	0.0	4.233184099483213E-5
6	0.0	0.0	0.0	0.0	4.233184099483213E-5
7	0.0	0.0	0.0	0.0	4.233184099483213E-5
8	0.0	0.0	0.0	8.466368198966426E-5	4.233184099483213E-5
9	0.0	0.0	0.0	8.466368198966426E-5	4.233184099483213E-5
10	0.0	0.0	0.0	8.466368198966426E-5	1.269955229844964E-4
11	4.233184099483213E-5	0.0	0.0	8.466368198966426E-5	1.269955229844964E-4
12	4.233184099483213E-5	0.0	0.0	1.269955229844964E-4	4.656502509431534E-4
13	4.233184099483213E-5	0.0	0.0	1.269955229844964E-4	5.503139329328177E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGGAC	230	0.0	13.218099	15
CGCATCG	375	0.0	12.667077	13
AATCGTA	270	0.0	12.667076	13
CGGTCCA	530	0.0	12.189849	10
AGGATCG	110	3.8187864E-8	12.090532	5
TCCAACG	255	0.0	11.924987	18
CGCCGGT	520	0.0	11.876391	7
CAACGGA	270	0.0	11.611241	14
GCATCGC	405	0.0	11.493955	14
CGCCAGT	415	0.0	11.449065	18
CCCCTAG	100	1.9448453E-6	11.393126	1
GATATAC	625	0.0	11.393126	1
GTCTAAA	110	5.010679E-7	11.220502	1
ACTGTTC	670	0.0	11.202805	8
GTCTTAA	85	5.358162E-5	11.16973	1
CGAACGA	190	0.0	11.001755	16
TCTAGAT	365	0.0	10.925839	2
TCGCCAG	435	0.0	10.922208	17
CGGACAT	235	0.0	10.914553	5
CGCGCTA	70	0.001489914	10.858875	16
>>END_MODULE