FastQCFastQC Report
Thu 2 Feb 2017
SRR4062342_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062342_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2362288
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA36440.15425722858516827No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC32380.13707050114126643No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC25480.10786153085483227No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA23800.10074978156770047No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG23750.1005381223627263No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA23670.10019946763476764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG8350.021.0781484
TTAGGAC6650.019.8499093
GTCCTAA5450.019.7838731
GGCGTTA6900.018.1737942
GGACGTG9350.018.1175256
CGATTAA3950.017.82266441
TCGTATA1002.4847835E-517.59988244
GTTATAT3400.017.4741691
ACGATTA4100.017.17061440
CGTAACG902.2166123E-417.11099615
AGGACGT9800.017.061115
GTATTAG10250.016.9595741
ATACCGT5800.016.6895436
GCTATCG1206.4121505E-616.49988713
CGTCTTA7350.016.46247315
GACGTGA6050.016.3635257
TACCGTC6100.016.2293997
TTAACGG7050.016.22684135
TAACGGC7100.016.11256836
ACCGTCG5750.016.0694588
Produced by FastQC (version 0.11.5)