##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062342_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2362288
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.199747448236625	32.0	32.0	32.0	32.0	32.0
2	31.413393286508672	32.0	32.0	32.0	32.0	32.0
3	31.512412119098094	32.0	32.0	32.0	32.0	32.0
4	31.62713521805978	32.0	32.0	32.0	32.0	32.0
5	31.551954291771366	32.0	32.0	32.0	32.0	32.0
6	35.15970448988438	36.0	36.0	36.0	36.0	36.0
7	35.17693312585087	36.0	36.0	36.0	36.0	36.0
8	35.12123204283305	36.0	36.0	36.0	36.0	36.0
9	35.2328602608996	36.0	36.0	36.0	36.0	36.0
10	35.11933007321715	36.0	36.0	36.0	36.0	36.0
11	35.24512040868853	36.0	36.0	36.0	36.0	36.0
12	35.15356129311921	36.0	36.0	36.0	36.0	36.0
13	35.19891732083472	36.0	36.0	36.0	36.0	36.0
14	35.15277265092148	36.0	36.0	36.0	36.0	36.0
15	35.12109742757869	36.0	36.0	36.0	36.0	36.0
16	35.128659587654006	36.0	36.0	36.0	36.0	36.0
17	35.103394674993055	36.0	36.0	36.0	36.0	36.0
18	35.114115213725	36.0	36.0	36.0	36.0	36.0
19	35.08988108139228	36.0	36.0	36.0	36.0	36.0
20	35.07173469111302	36.0	36.0	36.0	36.0	36.0
21	35.05143995990328	36.0	36.0	36.0	36.0	36.0
22	35.03013688424104	36.0	36.0	36.0	36.0	36.0
23	34.99011255189884	36.0	36.0	36.0	36.0	36.0
24	34.96069192240743	36.0	36.0	36.0	36.0	36.0
25	34.940267232445834	36.0	36.0	36.0	32.0	36.0
26	34.886765288567695	36.0	36.0	36.0	32.0	36.0
27	34.86374438679788	36.0	36.0	36.0	32.0	36.0
28	34.836307850693906	36.0	36.0	36.0	32.0	36.0
29	34.80089557242809	36.0	36.0	36.0	32.0	36.0
30	34.780090319215944	36.0	36.0	36.0	32.0	36.0
31	34.7795776806215	36.0	36.0	36.0	32.0	36.0
32	34.72681146413985	36.0	36.0	36.0	32.0	36.0
33	34.6938700954329	36.0	36.0	36.0	32.0	36.0
34	34.66983788598173	36.0	36.0	36.0	32.0	36.0
35	34.61512694472478	36.0	36.0	36.0	32.0	36.0
36	34.58771496108857	36.0	36.0	36.0	32.0	36.0
37	34.56075889137988	36.0	36.0	36.0	32.0	36.0
38	34.51132800065022	36.0	36.0	36.0	32.0	36.0
39	34.44736628218067	36.0	36.0	36.0	32.0	36.0
40	34.4086542369093	36.0	36.0	36.0	32.0	36.0
41	34.382928753818334	36.0	36.0	36.0	32.0	36.0
42	34.30403955825877	36.0	36.0	36.0	32.0	36.0
43	34.31007734873987	36.0	36.0	36.0	32.0	36.0
44	34.271075753676094	36.0	36.0	36.0	32.0	36.0
45	34.169320590884766	36.0	36.0	36.0	32.0	36.0
46	34.20505205123169	36.0	36.0	36.0	32.0	36.0
47	34.13341980317387	36.0	36.0	36.0	32.0	36.0
48	34.095249605467245	36.0	36.0	36.0	32.0	36.0
49	34.097210839660534	36.0	36.0	36.0	32.0	36.0
50	33.57574309313682	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	18.0
21	57.0
22	241.0
23	714.0
24	2033.0
25	4722.0
26	9788.0
27	18231.0
28	29918.0
29	42639.0
30	58372.0
31	80911.0
32	117526.0
33	179365.0
34	391929.0
35	1425820.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.560689774887486	18.306131003035652	12.402188041135851	25.73099118094101
2	15.678017763252589	20.552709785029084	37.095775935788126	26.6734965159302
3	18.58229397772746	24.50944658996961	28.50109703291014	28.40716239939279
4	12.12766452820045	16.352810943641433	35.72955845717176	35.78996607098636
5	14.306638309977446	36.978260059738695	33.15053033330398	15.564571296979876
6	34.03969983198414	35.252978376371615	17.099307151532187	13.608014640112062
7	30.229489570695506	30.687351150538078	20.57769465678584	18.505464621980572
8	28.57987679740997	32.32163055478418	19.517560940918298	19.58093170688756
9	27.309625244678042	14.832992420907187	18.906881802726847	38.95050053168792
10	16.448206145906003	26.438774611732352	31.04392859803716	26.069090644324483
11	36.81164193358303	21.30667386872388	22.28589401461634	19.595790183076748
12	24.906912281652364	24.080129095182297	28.30167193839193	22.71128668477341
13	29.369746618532545	19.50680865330561	25.71096326950821	25.412481458653644
14	23.78638845051916	20.23969981644914	24.977098474021798	30.996813259009908
15	25.689247035077855	26.96411275847822	22.987205624377722	24.3594345820662
16	25.831228029774522	25.730689907411797	24.324341485881483	24.113740576932194
17	23.65071490013072	25.74080721740956	25.869242022987883	24.739235859471833
18	24.830545640497686	24.302709915133125	27.404871886916414	23.461872557452775
19	25.199890953177594	25.604498689406203	25.71066694662124	23.48494341079496
20	25.157559112182764	24.232735381968666	26.25425011683588	24.355455389012686
21	26.475010667623934	24.35460875219279	24.532106161484123	24.63827441869916
22	25.133980276748645	24.787621153728928	25.153960905698202	24.924437663824225
23	24.06954613493359	24.479868669696497	25.63197205421185	25.818613141158064
24	24.273204621959728	25.85302892788686	25.327140467208064	24.546625982945347
25	24.909282864748075	24.40417933799774	25.621050439235184	25.065487358019006
26	23.712858042711137	25.30665185616656	26.600143589604652	24.380346511517647
27	25.08889686608915	25.25877454400141	25.462263703663567	24.190064886245878
28	24.098077795764105	24.503405173289625	26.278887248294875	25.119629782651398
29	23.95503850504257	24.95034475051306	26.433567795289992	24.66104894915438
30	24.41637090820425	24.83812304003576	26.594513454752345	24.150992597007647
31	24.525163739560966	24.732039446502714	25.46967177583766	25.27312503809866
32	23.78833571520492	25.34911069268438	25.48101670922428	25.381536882886422
33	23.76932871859824	24.957033181390244	25.934348394437933	25.33928970557358
34	24.256187221879806	25.090209153159986	26.636548972860208	24.017054652099997
35	25.461417066843673	24.9074202637443	25.797320225137664	23.833842444274364
36	24.030770168582325	25.36523912410341	25.666218513576666	24.937772193737597
37	25.284469971485272	25.160183686324444	25.32510853884031	24.230237803349972
38	24.4159475897943	25.065487358019006	25.590867836605867	24.927697215580828
39	24.466068489532184	25.033399822544922	25.381790873932392	25.118740813990502
40	25.23125884735477	25.30000575713037	25.58591501120947	23.882820384305386
41	23.840403879628564	25.184016512804536	26.583676503457664	24.391903104109236
42	25.52131662185136	25.80946946350318	25.271812751027817	23.397401163617644
43	24.183545782732672	24.58150741992509	25.711132596872183	25.523814200470053
44	23.974172497172233	25.106549243783995	25.96444633338526	24.954831925658514
45	24.164708113489972	25.30682118353054	25.896165073860594	24.63230562911889
46	24.231909260775367	24.752506682086757	25.933693041401423	25.081891015736453
47	24.736367765644058	24.81794125777266	26.033712245802477	24.411978730780806
48	24.906827617970375	26.145118630751202	24.526264367426833	24.42178938385159
49	23.713037408240403	25.42675805268369	26.140600189562065	24.71960434951384
50	23.478835110920144	26.42771723140942	24.826640308006866	25.266807349663566
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	71.0
1	71.5
2	72.0
3	247.0
4	422.0
5	457.0
6	492.0
7	459.5
8	427.0
9	590.0
10	753.0
11	1195.0
12	1637.0
13	2908.0
14	4179.0
15	5710.5
16	7242.0
17	8701.5
18	10161.0
19	11045.5
20	11930.0
21	12911.5
22	13893.0
23	13859.0
24	13825.0
25	15694.5
26	17564.0
27	23189.0
28	28814.0
29	33300.5
30	37787.0
31	39543.5
32	41300.0
33	48107.0
34	54914.0
35	63641.0
36	72368.0
37	86245.5
38	100123.0
39	104434.5
40	108746.0
41	117533.5
42	126321.0
43	125441.0
44	124561.0
45	138961.5
46	153362.0
47	163842.5
48	174323.0
49	173195.5
50	172068.0
51	161903.0
52	151738.0
53	148577.0
54	145416.0
55	149509.0
56	153602.0
57	151269.5
58	148937.0
59	137901.0
60	126865.0
61	113073.0
62	99281.0
63	87026.5
64	74772.0
65	64633.5
66	54495.0
67	47540.0
68	40585.0
69	37168.0
70	33751.0
71	26421.5
72	19092.0
73	16951.5
74	14811.0
75	10809.5
76	6808.0
77	6053.5
78	5299.0
79	4568.5
80	3838.0
81	3007.0
82	2176.0
83	1912.0
84	1648.0
85	1372.5
86	1097.0
87	791.5
88	486.0
89	312.0
90	138.0
91	95.0
92	52.0
93	35.0
94	18.0
95	14.0
96	10.0
97	10.0
98	10.0
99	9.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015154799076149902
2	0.00457183882744187
3	2.963228869638249E-4
4	1.269955229844964E-4
5	0.0
6	4.656502509431534E-4
7	8.466368198966426E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	8.466368198966426E-5
47	4.233184099483213E-5
48	0.0
49	4.233184099483213E-5
50	1.269955229844964E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2362288.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.695808864185594
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.81833392519451	28.413135726356003
2	15.587647808970328	12.687038717521956
3	5.8612529215113	7.155852857954198
4	2.872588365237468	4.676092282287494
5	1.5651701992850418	3.184793363501167
6	0.9784064222125424	2.389022444991198
7	0.6458482056919734	1.8398320593882442
8	0.4356944137751545	1.4184749268949663
9	0.3227925330767688	1.1822672905990639
>10	1.626799487891796	11.859097111666674
>50	0.12915101827968214	3.681444884995743
>100	0.12878427969610648	11.16973594122189
>500	0.019574860783950217	5.494086088453024
>1k	0.007955558393477087	4.849126304168467
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	3644	0.15425722858516827	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	3238	0.13707050114126643	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2548	0.10786153085483227	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	2380	0.10074978156770047	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	2375	0.1005381223627263	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2367	0.10019946763476764	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	4.233184099483213E-5
6	0.0	0.0	0.0	0.0	4.233184099483213E-5
7	0.0	0.0	0.0	0.0	4.233184099483213E-5
8	0.0	0.0	0.0	0.0	4.233184099483213E-5
9	0.0	0.0	0.0	0.0	4.233184099483213E-5
10	0.0	0.0	0.0	8.466368198966426E-5	4.233184099483213E-5
11	0.0	0.0	0.0	8.466368198966426E-5	4.233184099483213E-5
12	0.0	0.0	0.0	1.269955229844964E-4	2.539910459689928E-4
13	0.0	0.0	0.0	1.269955229844964E-4	3.809865689534892E-4
14	0.0	0.0	0.0	2.1165920497416064E-4	3.809865689534892E-4
15	0.0	0.0	0.0	2.539910459689928E-4	4.233184099483213E-4
16	0.0	0.0	0.0	4.656502509431534E-4	4.233184099483213E-4
17	0.0	0.0	0.0	5.926457739276498E-4	4.233184099483213E-4
18	0.0	0.0	0.0	9.313005018863069E-4	4.233184099483213E-4
19	0.0	0.0	0.0	0.0010582960248708031	4.233184099483213E-4
20	0.0	0.0	0.0	0.0014816144348191245	4.656502509431534E-4
21	0.0	0.0	0.0	0.001947264685762278	5.079820919379856E-4
22	0.0	0.0	0.0	0.0030478925516279134	5.079820919379856E-4
23	0.0	0.0	0.0	0.006222780626240323	5.079820919379856E-4
24	0.0	0.0	0.0	0.009820987110801054	5.079820919379856E-4
25	0.0	0.0	0.0	0.012360897570490982	5.503139329328177E-4
26	0.0	0.0	0.0	0.01553578564510339	5.926457739276498E-4
27	0.0	0.0	0.0	0.02027695183652459	5.926457739276498E-4
28	0.0	0.0	0.0	0.026161077734806255	6.34977614922482E-4
29	0.0	0.0	0.0	0.037040360870478115	6.34977614922482E-4
30	0.0	0.0	0.0	0.05748664007098203	6.34977614922482E-4
31	0.0	0.0	0.0	0.09672825667319142	7.196412969121462E-4
32	0.0	0.0	0.0	0.14464790067934138	7.619731379069784E-4
33	4.233184099483213E-5	0.0	0.0	0.19307552677742934	7.619731379069784E-4
34	4.233184099483213E-5	0.0	0.0	0.2506044986894062	8.043049789018105E-4
35	4.233184099483213E-5	0.0	0.0	0.31342495072573706	8.043049789018105E-4
36	4.233184099483213E-5	0.0	0.0	0.4079096198262024	8.043049789018105E-4
37	4.233184099483213E-5	0.0	0.0	0.5385456811362543	8.043049789018105E-4
38	4.233184099483213E-5	0.0	0.0	0.7113865879181539	8.043049789018105E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACG	835	0.0	21.078148	4
TTAGGAC	665	0.0	19.849909	3
GTCCTAA	545	0.0	19.783873	1
GGCGTTA	690	0.0	18.17379	42
GGACGTG	935	0.0	18.117525	6
CGATTAA	395	0.0	17.822664	41
TCGTATA	100	2.4847835E-5	17.599882	44
GTTATAT	340	0.0	17.474169	1
ACGATTA	410	0.0	17.170614	40
CGTAACG	90	2.2166123E-4	17.110996	15
AGGACGT	980	0.0	17.06111	5
GTATTAG	1025	0.0	16.959574	1
ATACCGT	580	0.0	16.689543	6
GCTATCG	120	6.4121505E-6	16.499887	13
CGTCTTA	735	0.0	16.462473	15
GACGTGA	605	0.0	16.363525	7
TACCGTC	610	0.0	16.229399	7
TTAACGG	705	0.0	16.226841	35
TAACGGC	710	0.0	16.112568	36
ACCGTCG	575	0.0	16.069458	8
>>END_MODULE