FastQCFastQC Report
Thu 2 Feb 2017
SRR4062341_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062341_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1565656
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG146530.9359016284547819No Hit
TCCATGTACTCTGCGTTGATACCAC125600.8022196446729037No Hit
GAGTACATGGAAGCAGTGGTATCAA52680.33647237962873067No Hit
CATGTACTCTGCGTTGATACCACTG47510.3034510773758731No Hit
GCTTCCATGTACTCTGCGTTGATAC40390.2579749319135238No Hit
GCGTTGATACCACTGCTTCCATGTA32880.2100078178092761No Hit
CCCATGTACTCTGCGTTGATACCAC31250.19959684630595736No Hit
CATGGAAGCAGTGGTATCAACGCAG30470.19461490902216066No Hit
GTATCAACGCAGAGTACTTTTTTTT27770.17736974150132595No Hit
GTACTTTTTTTTTTTTTTTTTTTTT27500.1756452247492425No Hit
GTACATGGGAAGCAGTGGTATCAAC26720.17066328746544582No Hit
GTATCAACGCAGAGTACATGGAAGC24760.15814457326513615No Hit
ACTCTGCGTTGATACCACTGCTTCC20530.13112714414916174No Hit
ACGCAGAGTACATGGAAGCAGTGGT20520.13106327315834385No Hit
TATCAACGCAGAGTACTTTTTTTTT20320.12978585334198572No Hit
CAGTGGTATCAACGCAGAGTACATG18940.12097165660911464No Hit
TATCAACGCAGAGTACATGGAAGCA17340.11075229807824963No Hit
GAGTACTTTTTTTTTTTTTTTTTTT16880.10781423250062594No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA551.1325896E-515.5392571
GTCGTGA400.005293106714.244321
TTGCGCG1650.013.82150718
CGCAAGA2150.013.6935262
CAAGACG2500.013.6784814
ACGAACG1051.369699E-913.57252415
CGATAAC1051.369699E-913.57165610
GCGTAAC852.701654E-713.41070511
AAGACGG2700.013.3701685
CGCAGTT500.001497197913.30277617
TCGCGTA957.385097E-813.0002189
CCGATAA1102.744855E-912.9547649
TAACGAA1102.746674E-912.954349513
GATAACG1102.748493E-912.95352211
ATTCCGA1207.403287E-1012.666886
ATTTGCG1750.012.48752216
CGCGTAA951.0395634E-612.00020210
AGACGGA2950.011.9154556
CGAACGA1209.98989E-911.876718516
AACGAAC1209.998985E-911.87595814
Produced by FastQC (version 0.11.5)