##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062341_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1565656
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.089312722590403	32.0	32.0	32.0	32.0	32.0
2	30.72445479722238	32.0	32.0	32.0	32.0	32.0
3	30.711978876585917	32.0	32.0	32.0	32.0	32.0
4	30.77180555626523	32.0	32.0	32.0	32.0	32.0
5	30.58017725477372	32.0	32.0	32.0	32.0	32.0
6	34.2268461271186	36.0	36.0	36.0	32.0	36.0
7	34.09309835621618	36.0	36.0	36.0	32.0	36.0
8	34.07286977471424	36.0	36.0	36.0	32.0	36.0
9	34.24026669970926	36.0	36.0	36.0	32.0	36.0
10	33.88978677308425	36.0	36.0	36.0	32.0	36.0
11	34.24556607581742	36.0	36.0	36.0	32.0	36.0
12	34.004695795244935	36.0	36.0	36.0	32.0	36.0
13	34.12620780043637	36.0	36.0	36.0	32.0	36.0
14	34.031018946690715	36.0	36.0	36.0	32.0	36.0
15	33.975098616809824	36.0	36.0	36.0	32.0	36.0
16	33.96012789527202	36.0	36.0	36.0	32.0	36.0
17	33.89283852902553	36.0	36.0	36.0	32.0	36.0
18	33.89985858962633	36.0	36.0	36.0	32.0	36.0
19	33.90431422994579	36.0	36.0	36.0	32.0	36.0
20	33.87584054223916	36.0	36.0	36.0	32.0	36.0
21	33.84563658939128	36.0	36.0	36.0	32.0	36.0
22	33.806029549275195	36.0	36.0	36.0	32.0	36.0
23	33.73121618031036	36.0	36.0	36.0	27.0	36.0
24	33.72717250788168	36.0	36.0	36.0	27.0	36.0
25	33.217553536664504	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	11.0
4	52.0
5	151.0
6	260.0
7	87.0
8	246.0
9	253.0
10	193.0
11	61.0
12	126.0
13	92.0
14	287.0
15	361.0
16	586.0
17	773.0
18	1074.0
19	1626.0
20	2642.0
21	3764.0
22	5765.0
23	8279.0
24	11260.0
25	15456.0
26	21122.0
27	26968.0
28	35684.0
29	46382.0
30	58893.0
31	79253.0
32	112226.0
33	160192.0
34	345472.0
35	626058.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.9848447587432	16.812825807984638	11.379717169446021	25.822612263826137
2	16.060181207903653	20.199603348226404	37.02287614053353	26.717339303336413
3	19.04195862262789	24.61008309966211	28.089793624128923	28.25816465358108
4	12.590646368012756	15.90016506710003	35.11540643301751	36.393782131869706
5	14.217303632674133	36.817372125474726	33.97658210702256	14.98874213482859
6	34.34898462560865	34.4356972152287	17.424374097409483	13.790944061753166
7	29.734554337363722	30.48413218851423	21.543971867821714	18.23734160630034
8	28.344806159526836	32.8087882189588	20.110517581296396	18.735888040217965
9	27.05141639138421	14.903292584923067	18.292863029208817	39.75242799448391
10	15.392889116255276	27.44295945773117	32.4449418084154	24.71920961759816
11	36.87134332767813	21.212328561260712	21.78914673755604	20.127181373505117
12	23.753663813640674	23.7951705132313	29.522199688795688	22.92896598433234
13	30.106223857298982	19.977835657732932	24.932007394935198	24.983933090032888
14	23.793615275554597	19.402366564963504	25.153082334794185	31.65093582468771
15	25.570840199273732	26.686015989728105	23.552589426675592	24.19055438432257
16	25.965446927034947	25.50359259114797	24.466514441660117	24.064446040156966
17	24.071166694058803	25.643645972492322	26.39455295070886	23.89063438274001
18	25.25342256365783	24.260197979782475	27.239104268755188	23.247275187804505
19	24.717640949289212	26.888575938276247	25.07079761475353	23.32298549768101
20	24.750563717684557	24.11272818895382	26.96552976672384	24.171178326637786
21	25.559021862033326	25.36971803963101	23.966823226044113	25.104436872291547
22	24.46226276298367	25.66867735765779	24.728684649877504	25.140375229481037
23	23.59090699768722	25.123633823860498	25.02289752143924	26.262561657013038
24	23.71526334303348	26.44482108023408	24.955006235809535	24.8849093409229
25	25.119882221872608	24.64048124286111	24.589637622488357	25.649998912777928
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	327.0
1	327.0
2	698.0
3	1069.0
4	1069.0
5	1069.0
6	2720.0
7	4371.0
8	4371.0
9	4371.0
10	4295.0
11	4219.0
12	4219.0
13	4219.0
14	4406.0
15	4593.0
16	4593.0
17	4593.0
18	7277.0
19	9961.0
20	9961.0
21	9961.0
22	15846.5
23	21732.0
24	21732.0
25	21732.0
26	32169.5
27	42607.0
28	42607.0
29	42607.0
30	53817.0
31	65027.0
32	65027.0
33	65027.0
34	79806.0
35	94585.0
36	94585.0
37	94585.0
38	108836.0
39	123087.0
40	123087.0
41	123087.0
42	144928.5
43	166770.0
44	166770.0
45	166770.0
46	210732.0
47	254694.0
48	254694.0
49	254694.0
50	234866.0
51	215038.0
52	215038.0
53	215038.0
54	194625.0
55	174212.0
56	174212.0
57	174212.0
58	158348.0
59	142484.0
60	142484.0
61	142484.0
62	124243.0
63	106002.0
64	106002.0
65	106002.0
66	86534.5
67	67067.0
68	67067.0
69	67067.0
70	49968.5
71	32870.0
72	32870.0
73	32870.0
74	25160.5
75	17451.0
76	17451.0
77	17451.0
78	13922.0
79	10393.0
80	10393.0
81	10393.0
82	7131.0
83	3869.0
84	3869.0
85	3869.0
86	2833.5
87	1798.0
88	1798.0
89	1798.0
90	1199.0
91	600.0
92	600.0
93	600.0
94	378.0
95	156.0
96	156.0
97	156.0
98	415.0
99	674.0
100	674.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007983873852238296
2	0.0032574205317132243
3	0.00421548539398182
4	0.014818069869754275
5	0.03372388315185456
6	0.04573162942562096
7	0.06655357243225843
8	0.08814196732871077
9	0.09612584118094906
10	0.1185445589580342
11	0.12167423750811161
12	0.13112714414916174
13	0.12026907571011769
14	0.12486778704900693
15	0.1134987506834196
16	0.1113271369956108
17	0.12435681912246369
18	0.13170198306652292
19	0.1305523052318006
20	0.12327101227855929
21	0.12978585334198572
22	0.1498413444588083
23	0.138472308093221
24	0.1345123066625108
25	0.13016907928689317
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1565656.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.3577813984036
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.3173715061059	41.788411885203615
2	13.26439192093558	14.155170490000629
3	3.8871226673755697	6.222247246638155
4	1.562223111452557	3.334270371056778
5	0.8141783097219788	2.172137413473354
6	0.4621207485542088	1.4794642728613265
7	0.30177754347146046	1.1271526136848202
8	0.21472369721538112	0.916574407766025
9	0.15457441688747275	0.742297315146073
>10	0.860844403130566	8.415144643457605
>50	0.07493487418230238	2.8055938126382625
>100	0.07372896540183545	8.108446044439237
>500	0.008285406540101327	3.1055925427997275
>1k	0.0033621939583019884	3.546402030833028
>5k	1.2007835565364244E-4	0.33752679984875417
>10k+	2.4015671130728488E-4	1.7435681101526477
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	14653	0.9359016284547819	No Hit
TCCATGTACTCTGCGTTGATACCAC	12560	0.8022196446729037	No Hit
GAGTACATGGAAGCAGTGGTATCAA	5268	0.33647237962873067	No Hit
CATGTACTCTGCGTTGATACCACTG	4751	0.3034510773758731	No Hit
GCTTCCATGTACTCTGCGTTGATAC	4039	0.2579749319135238	No Hit
GCGTTGATACCACTGCTTCCATGTA	3288	0.2100078178092761	No Hit
CCCATGTACTCTGCGTTGATACCAC	3125	0.19959684630595736	No Hit
CATGGAAGCAGTGGTATCAACGCAG	3047	0.19461490902216066	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2777	0.17736974150132595	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2750	0.1756452247492425	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2672	0.17066328746544582	No Hit
GTATCAACGCAGAGTACATGGAAGC	2476	0.15814457326513615	No Hit
ACTCTGCGTTGATACCACTGCTTCC	2053	0.13112714414916174	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	2052	0.13106327315834385	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2032	0.12978585334198572	No Hit
CAGTGGTATCAACGCAGAGTACATG	1894	0.12097165660911464	No Hit
TATCAACGCAGAGTACATGGAAGCA	1734	0.11075229807824963	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1688	0.10781423250062594	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.277419816358127E-4	0.0
5	0.0	0.0	0.0	1.277419816358127E-4	6.387099081790636E-5
6	0.0	0.0	0.0	1.9161297245371908E-4	6.387099081790636E-5
7	0.0	0.0	0.0	2.554839632716254E-4	6.387099081790636E-5
8	0.0	0.0	0.0	2.554839632716254E-4	6.387099081790636E-5
9	0.0	0.0	0.0	3.193549540895318E-4	6.387099081790636E-5
10	0.0	0.0	0.0	3.193549540895318E-4	6.387099081790636E-5
11	0.0	0.0	0.0	3.193549540895318E-4	6.387099081790636E-5
12	0.0	0.0	0.0	3.193549540895318E-4	3.193549540895318E-4
13	0.0	0.0	0.0	3.193549540895318E-4	5.109679265432508E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAA	55	1.1325896E-5	15.539257	1
GTCGTGA	40	0.0052931067	14.24432	1
TTGCGCG	165	0.0	13.821507	18
CGCAAGA	215	0.0	13.693526	2
CAAGACG	250	0.0	13.678481	4
ACGAACG	105	1.369699E-9	13.572524	15
CGATAAC	105	1.369699E-9	13.571656	10
GCGTAAC	85	2.701654E-7	13.410705	11
AAGACGG	270	0.0	13.370168	5
CGCAGTT	50	0.0014971979	13.302776	17
TCGCGTA	95	7.385097E-8	13.000218	9
CCGATAA	110	2.744855E-9	12.954764	9
TAACGAA	110	2.746674E-9	12.9543495	13
GATAACG	110	2.748493E-9	12.953522	11
ATTCCGA	120	7.403287E-10	12.66688	6
ATTTGCG	175	0.0	12.487522	16
CGCGTAA	95	1.0395634E-6	12.000202	10
AGACGGA	295	0.0	11.915455	6
CGAACGA	120	9.98989E-9	11.8767185	16
AACGAAC	120	9.998985E-9	11.875958	14
>>END_MODULE