FastQCFastQC Report
Thu 2 Feb 2017
SRR4062341_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062341_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1565656
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA96010.613225382842719No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC70260.44875758148661005No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC30000.19161297245371908No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA27850.1778807094278692No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG25550.16319038153975074No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA20710.13227682198388407No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC18310.11694778418758654No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG17730.11324326672014796No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGTA852.3536995E-723.29398342
ATTCGTG1550.022.70954544
TCGTATA500.002580861821.99987244
CGCGCGA550.004484344319.99988410
AACGAAT1051.7934763E-618.85703331
TAGGACG3150.018.8570334
CGTCGTA3400.018.76459710
GTCGTAT1203.163641E-718.33322743
ATACCGT3600.018.3332276
GATATAC2350.017.790541
GTATAGG2003.6379788E-1217.6032711
GTATAGA2003.6379788E-1217.6032711
TACGCTG750.001291151417.5998975
TACCGTC3500.017.5998977
GTCGTAG3500.016.9713311
TATGGCG4300.016.88362116
GTATTAC1702.4629117E-916.8266561
TCCAACG1451.2499913E-716.68955828
AATCACG2650.016.60367834
CCGTCGT3600.016.4999059
Produced by FastQC (version 0.11.5)