##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062341_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1565656
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28469791576183	32.0	32.0	32.0	32.0	32.0
2	31.430358265161697	32.0	32.0	32.0	32.0	32.0
3	31.50984571323458	32.0	32.0	32.0	32.0	32.0
4	31.610091233323285	32.0	32.0	32.0	32.0	32.0
5	31.558859672878334	32.0	32.0	32.0	32.0	32.0
6	35.14175910928071	36.0	36.0	36.0	36.0	36.0
7	35.16417463350825	36.0	36.0	36.0	36.0	36.0
8	35.10794708416153	36.0	36.0	36.0	36.0	36.0
9	35.21865020157685	36.0	36.0	36.0	36.0	36.0
10	35.11441785424129	36.0	36.0	36.0	36.0	36.0
11	35.233040974518026	36.0	36.0	36.0	36.0	36.0
12	35.14940446688161	36.0	36.0	36.0	36.0	36.0
13	35.18304404032559	36.0	36.0	36.0	36.0	36.0
14	35.14321728400108	36.0	36.0	36.0	36.0	36.0
15	35.113286060284	36.0	36.0	36.0	36.0	36.0
16	35.120460049972664	36.0	36.0	36.0	36.0	36.0
17	35.09871261630907	36.0	36.0	36.0	36.0	36.0
18	35.10558513492108	36.0	36.0	36.0	36.0	36.0
19	35.082896881562746	36.0	36.0	36.0	36.0	36.0
20	35.07694921489778	36.0	36.0	36.0	36.0	36.0
21	35.04981554057852	36.0	36.0	36.0	36.0	36.0
22	35.03309092163285	36.0	36.0	36.0	36.0	36.0
23	34.9732463580761	36.0	36.0	36.0	36.0	36.0
24	34.95781129443505	36.0	36.0	36.0	36.0	36.0
25	34.93132335583295	36.0	36.0	36.0	32.0	36.0
26	34.88566390062696	36.0	36.0	36.0	32.0	36.0
27	34.862381647054015	36.0	36.0	36.0	32.0	36.0
28	34.83353686889074	36.0	36.0	36.0	32.0	36.0
29	34.81464191367708	36.0	36.0	36.0	32.0	36.0
30	34.791162298742506	36.0	36.0	36.0	32.0	36.0
31	34.768822780994036	36.0	36.0	36.0	32.0	36.0
32	34.727728185501796	36.0	36.0	36.0	32.0	36.0
33	34.70004969163086	36.0	36.0	36.0	32.0	36.0
34	34.67863119356998	36.0	36.0	36.0	32.0	36.0
35	34.615084667385425	36.0	36.0	36.0	32.0	36.0
36	34.58792352854012	36.0	36.0	36.0	32.0	36.0
37	34.548620514340314	36.0	36.0	36.0	32.0	36.0
38	34.48845659582948	36.0	36.0	36.0	32.0	36.0
39	34.41255869744056	36.0	36.0	36.0	32.0	36.0
40	34.33184301021425	36.0	36.0	36.0	32.0	36.0
41	34.34021713582038	36.0	36.0	36.0	32.0	36.0
42	34.2357561303377	36.0	36.0	36.0	32.0	36.0
43	34.23375888445482	36.0	36.0	36.0	32.0	36.0
44	34.23756495679766	36.0	36.0	36.0	32.0	36.0
45	34.090007638970505	36.0	36.0	36.0	32.0	36.0
46	34.16907162237426	36.0	36.0	36.0	32.0	36.0
47	34.105233844471584	36.0	36.0	36.0	32.0	36.0
48	34.09171363313525	36.0	36.0	36.0	32.0	36.0
49	34.123511167204036	36.0	36.0	36.0	32.0	36.0
50	33.54892134670707	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	7.0
21	32.0
22	167.0
23	433.0
24	1235.0
25	2808.0
26	5876.0
27	10736.0
28	18615.0
29	27674.0
30	40077.0
31	57493.0
32	84654.0
33	125322.0
34	257236.0
35	933286.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.518006715147926	17.369568217010688	11.865536061979691	26.2468890058617
2	15.538772358227298	20.6947298361573	36.672826230672584	27.093671574942817
3	18.50621500248779	24.846980194130605	28.430482733336444	28.216322070045162
4	12.379123841057911	16.239751873340946	35.30976232266174	36.0713619629394
5	13.980018599232528	37.01726305139827	33.875129658111355	15.12758869125785
6	34.583272123399226	34.57618241624884	17.120556957174337	13.719988503177593
7	29.69355976025385	30.501655536082	21.51149422350759	18.29329048015656
8	28.339494754914234	32.83888670308165	20.025663364110635	18.795955177893482
9	27.201569182502418	14.568462037637897	18.289458220707484	39.9405105591522
10	15.41072879355363	27.44421507661964	32.5237472343861	24.621308895440635
11	37.134594061530755	21.03163146949266	21.781093675749975	20.05268079322661
12	23.733885349016642	23.905506701344358	29.442929992284384	22.917677957354616
13	30.367462584373577	19.73786067948515	24.921247068321524	24.97342966781975
14	23.894009922997135	19.198725645991203	25.125634238938822	31.781630192072846
15	25.657551850470345	26.70880448834227	23.425260721384518	24.20838293980287
16	26.099730719902713	25.275539454388447	24.52550240921377	24.09922741649507
17	24.29620555217749	25.489507273628437	26.34767790625782	23.86660926793625
18	25.51837696147813	23.989497054269904	27.227181449820392	23.26494453443157
19	24.89046125074729	26.600734771878372	25.11803359103149	23.390770386342847
20	24.96065546965617	23.866545396945433	27.011552984819144	24.161246148579256
21	25.70417767376742	25.21805556265233	24.12228484418033	24.95548191939992
22	24.636190836301207	25.344903350416693	24.906556740433402	25.1123490728487
23	23.731458251365563	24.79835928198787	25.07421809133041	26.39596437531616
24	23.90014153811565	26.162771387839985	25.00255483963272	24.93453223441165
25	25.28888849146939	24.26031005533783	24.82703735686511	25.623764096327672
26	23.867120235862796	24.93038062000848	25.727298972443503	25.475200171685223
27	25.571453754847806	25.448310484550884	24.99897806414691	23.981257696454396
28	24.67847343222266	23.99971641280077	25.592722794790173	25.729087360186405
29	23.993457055700613	25.556571813987233	26.28284885057765	24.167122279734503
30	25.418227247875652	24.623927606064168	25.938264855115044	24.019580290945136
31	24.1542842105801	24.90847287015794	25.31724721139254	25.61999570786942
32	24.10733903232894	25.476094365556673	25.24801105734593	25.168555544768456
33	23.74461567547405	24.87525995493263	25.604730541063937	25.77539382852938
34	23.994351249572066	25.553825361382067	26.34141854915767	24.1104048398882
35	25.6181434491357	24.841025103854232	25.599046022881144	23.941785424128927
36	24.54728241708268	25.55491116822597	25.436430480258753	24.461375934432596
37	25.730748005947667	25.27783881005789	25.2136484642859	23.777764719708543
38	24.598762435681913	25.328871731721396	24.772172175752527	25.30019365684416
39	24.49145917110783	25.083926481934725	25.549865359951358	24.874748987006086
40	25.421229184444094	25.076772930963127	24.89199415452692	24.610003730065863
41	23.671866616932455	25.32344269750188	26.110652659332573	24.894038026233094
42	26.228430766400795	25.3520569013883	25.096700680098312	23.322811652112595
43	25.07159938070687	24.03503707072307	25.078752931678476	25.814610616891574
44	24.692333437230147	24.67029794539797	26.139266863218996	24.49810175415289
45	25.010219358530865	24.953757402647835	25.487144046968176	24.548879191853125
46	24.198879571500928	24.67874385798623	26.331091667300054	24.791284903212784
47	25.15087934442773	24.208526144010015	26.489169069814235	24.151425441748025
48	24.633188899732765	26.72847675351418	24.58847920616023	24.049855140592825
49	23.886537017071436	24.86146382091596	26.72892385044991	24.523075311562692
50	23.833013126766033	26.709123843296357	24.36595267415064	25.09191035578697
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	34.0
1	36.5
2	39.0
3	184.0
4	329.0
5	368.5
6	408.0
7	367.0
8	326.0
9	437.0
10	548.0
11	772.5
12	997.0
13	1709.5
14	2422.0
15	3112.5
16	3803.0
17	4260.5
18	4718.0
19	5106.0
20	5494.0
21	6198.0
22	6902.0
23	7438.5
24	7975.0
25	8825.0
26	9675.0
27	11825.5
28	13976.0
29	16539.5
30	19103.0
31	21592.5
32	24082.0
33	28480.0
34	32878.0
35	37413.5
36	41949.0
37	49063.0
38	56177.0
39	60721.0
40	65265.0
41	71772.0
42	78279.0
43	83025.0
44	87771.0
45	103794.5
46	119818.0
47	131223.0
48	142628.0
49	138481.0
50	134334.0
51	123718.0
52	113102.0
53	109026.5
54	104951.0
55	104856.5
56	104762.0
57	101333.5
58	97905.0
59	89291.0
60	80677.0
61	71013.0
62	61349.0
63	53310.5
64	45272.0
65	38388.0
66	31504.0
67	26769.5
68	22035.0
69	19945.5
70	17856.0
71	13717.0
72	9578.0
73	8376.5
74	7175.0
75	5066.5
76	2958.0
77	2708.0
78	2458.0
79	2102.0
80	1746.0
81	1353.0
82	960.0
83	823.5
84	687.0
85	574.5
86	462.0
87	333.5
88	205.0
89	127.5
90	50.0
91	32.0
92	14.0
93	10.0
94	6.0
95	5.0
96	4.0
97	5.5
98	7.0
99	5.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015329037796297525
2	0.004407098366435539
3	5.748389173611572E-4
4	2.554839632716254E-4
5	0.0
6	3.8322594490743816E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	3.193549540895318E-4
47	6.387099081790636E-5
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1565656.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.44274678670358
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.62728604268341	44.37939996632892
2	13.156199309886746	14.851440526466837
3	3.902061462639092	6.6072920124567736
4	1.521424397065789	3.434934879947899
5	0.7435967130392989	2.0985320492751116
6	0.4566011124322976	1.5463092582926008
7	0.29367337010618677	1.1603012166830968
8	0.20797798250646438	0.9391078883057467
9	0.14931782861088022	0.7585117551921883
>10	0.7944833806609741	8.211859056467706
>50	0.0710026470943476	2.811377755790552
>100	0.06762453897986674	7.7780856724465774
>500	0.006364519487084357	2.468173301306253
>1k	0.0021593905402607643	1.888079446339197
>5k	2.273042673958699E-4	1.0665952147005684
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	9601	0.613225382842719	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	7026	0.44875758148661005	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	3000	0.19161297245371908	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	2785	0.1778807094278692	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	2555	0.16319038153975074	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	2071	0.13227682198388407	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	1831	0.11694778418758654	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	1773	0.11324326672014796	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	6.387099081790636E-5	6.387099081790636E-5
7	0.0	0.0	0.0	1.277419816358127E-4	6.387099081790636E-5
8	0.0	0.0	0.0	1.277419816358127E-4	6.387099081790636E-5
9	0.0	0.0	0.0	1.277419816358127E-4	6.387099081790636E-5
10	0.0	0.0	0.0	1.9161297245371908E-4	1.277419816358127E-4
11	0.0	0.0	0.0	1.9161297245371908E-4	1.277419816358127E-4
12	0.0	0.0	0.0	1.9161297245371908E-4	3.8322594490743816E-4
13	0.0	0.0	0.0	1.9161297245371908E-4	4.470969357253445E-4
14	0.0	0.0	0.0	1.9161297245371908E-4	4.470969357253445E-4
15	0.0	0.0	0.0	1.9161297245371908E-4	6.387099081790636E-4
16	0.0	0.0	0.0	1.9161297245371908E-4	7.025808989969699E-4
17	0.0	0.0	0.0	3.8322594490743816E-4	7.025808989969699E-4
18	0.0	0.0	0.0	5.109679265432508E-4	7.664518898148763E-4
19	0.0	0.0	0.0	5.109679265432508E-4	7.664518898148763E-4
20	0.0	0.0	0.0	5.748389173611572E-4	7.664518898148763E-4
21	0.0	0.0	0.0	6.387099081790636E-4	7.664518898148763E-4
22	0.0	0.0	0.0	0.001085806843904408	8.303228806327827E-4
23	0.0	0.0	0.0	0.0024270976510804418	8.303228806327827E-4
24	0.0	0.0	0.0	0.00351290449498485	8.94193871450689E-4
25	0.0	0.0	0.0	0.00421548539398182	8.94193871450689E-4
26	0.0	0.0	0.0	0.00542903421952204	0.0010219358530865017
27	0.0	0.0	0.0	0.007536776916512951	0.001085806843904408
28	0.0	0.0	0.0	0.011177423393133613	0.001085806843904408
29	0.0	0.0	0.0	0.016478715631019842	0.001085806843904408
30	0.0	0.0	0.0	0.026442590198613232	0.001085806843904408
31	0.0	0.0	0.0	0.05039421175532812	0.001085806843904408
32	0.0	0.0	0.0	0.0802219644672904	0.0011496778347223145
33	0.0	0.0	0.0	0.10953874925270941	0.0011496778347223145
34	0.0	0.0	0.0	0.14696714987200254	0.0011496778347223145
35	0.0	0.0	0.0	0.1908465205639042	0.0011496778347223145
36	0.0	0.0	0.0	0.25880525479415656	0.0011496778347223145
37	0.0	0.0	0.0	0.367641423147869	0.0011496778347223145
38	0.0	0.0	0.0	0.49544727577449965	0.0013412908071760337
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTCGTA	85	2.3536995E-7	23.293983	42
ATTCGTG	155	0.0	22.709545	44
TCGTATA	50	0.0025808618	21.999872	44
CGCGCGA	55	0.0044843443	19.999884	10
AACGAAT	105	1.7934763E-6	18.857033	31
TAGGACG	315	0.0	18.857033	4
CGTCGTA	340	0.0	18.764597	10
GTCGTAT	120	3.163641E-7	18.333227	43
ATACCGT	360	0.0	18.333227	6
GATATAC	235	0.0	17.79054	1
GTATAGG	200	3.6379788E-12	17.603271	1
GTATAGA	200	3.6379788E-12	17.603271	1
TACGCTG	75	0.0012911514	17.599897	5
TACCGTC	350	0.0	17.599897	7
GTCGTAG	350	0.0	16.97133	11
TATGGCG	430	0.0	16.883621	16
GTATTAC	170	2.4629117E-9	16.826656	1
TCCAACG	145	1.2499913E-7	16.689558	28
AATCACG	265	0.0	16.603678	34
CCGTCGT	360	0.0	16.499905	9
>>END_MODULE