FastQCFastQC Report
Thu 2 Feb 2017
SRR4062340_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062340_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1277527
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT18740.14668965900524997No Hit
GCGCAAGACGGACCAGAGCGAAAGC14730.11530088992248305No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG12890.1008980632111885No Hit
TATCAACGCAGAGTACTTTTTTTTT12870.10074151074693528No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTCT2750.015.8918612
CGGTCGG1351.8189894E-1213.37064610
AAGACGG2450.013.1829125
GCGGTCG1403.6379788E-1212.8926189
GGTTCTA3350.012.478847513
GCGTAAC1301.9826984E-1012.42284611
CGAACGA1155.298716E-912.39447516
CGCGCTA1001.4368197E-712.3531616
GCGCGCT1001.4382931E-712.35219215
GACTTAG550.003068354412.0906857
CTATTTT3750.011.9101817
CAAGACG2650.011.8285784
CGCAAGA2900.011.787342
ACCCGCA658.02294E-411.6920917
CGCGTAT658.02294E-411.6920917
AACGGAC907.442799E-611.61317215
CGCGTAA1406.7848305E-1011.53595310
GGTCGGC1657.2759576E-1211.51493711
CGGTCCA2150.011.48860910
GTCCTAA3650.011.4464591
Produced by FastQC (version 0.11.5)