##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062340_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1277527
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.06409336162758	32.0	32.0	32.0	32.0	32.0
2	30.70390606225935	32.0	32.0	32.0	32.0	32.0
3	30.738459539406996	32.0	32.0	32.0	32.0	32.0
4	30.590915886709244	32.0	32.0	32.0	32.0	32.0
5	30.635952116863283	32.0	32.0	32.0	32.0	32.0
6	33.983502501317	36.0	36.0	36.0	32.0	36.0
7	33.97608426279836	36.0	36.0	36.0	32.0	36.0
8	33.908488039783116	36.0	36.0	36.0	32.0	36.0
9	34.092902928861776	36.0	36.0	36.0	32.0	36.0
10	33.74040392101302	36.0	36.0	36.0	32.0	36.0
11	34.13948198355103	36.0	36.0	36.0	32.0	36.0
12	33.88210894955645	36.0	36.0	36.0	32.0	36.0
13	34.02758063038981	36.0	36.0	36.0	32.0	36.0
14	33.87199722589033	36.0	36.0	36.0	32.0	36.0
15	33.79212728967764	36.0	36.0	36.0	32.0	36.0
16	33.83398707033198	36.0	36.0	36.0	32.0	36.0
17	33.73615273884622	36.0	36.0	36.0	32.0	36.0
18	33.78096431621406	36.0	36.0	36.0	32.0	36.0
19	33.74147004329458	36.0	36.0	36.0	32.0	36.0
20	33.68816079816708	36.0	36.0	36.0	32.0	36.0
21	33.66806572385554	36.0	36.0	36.0	32.0	36.0
22	33.629541293452114	36.0	36.0	36.0	27.0	36.0
23	33.59397335633611	36.0	36.0	36.0	27.0	36.0
24	33.588671707134175	36.0	36.0	36.0	27.0	36.0
25	32.858607293622754	36.0	32.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	11.0
4	50.0
5	129.0
6	204.0
7	50.0
8	189.0
9	215.0
10	139.0
11	70.0
12	105.0
13	67.0
14	337.0
15	640.0
16	929.0
17	1249.0
18	1567.0
19	1997.0
20	2345.0
21	3136.0
22	4113.0
23	5606.0
24	7453.0
25	10386.0
26	14031.0
27	19143.0
28	26900.0
29	37177.0
30	51628.0
31	76219.0
32	117714.0
33	176473.0
34	352011.0
35	365244.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.01260347965634	18.145566275906607	11.45280544846077	26.38902479597628
2	16.432703996843795	20.003162468698214	37.44339455006446	26.120738984393533
3	18.956370624635507	23.828206643626242	28.504655704852972	28.710767026885275
4	12.505763935807174	15.68643876057961	35.96022669001729	35.84757061359592
5	14.48477345567502	36.65348815539211	33.66682875530801	15.194909633624858
6	33.85154406018052	35.57728601880407	17.018492385810653	13.55267753520476
7	29.818960257104166	30.61516541720289	21.227307369656163	18.338566956036786
8	27.651796773336823	33.37644847688117	19.76760128076263	19.20415346901938
9	27.283696506377435	14.305839471533663	19.271141434948134	39.13932258714077
10	15.891607733975324	26.845251529742203	31.734784080650012	25.52835665563246
11	36.96807072511107	21.092537140322516	22.808393787337224	19.130998347229202
12	24.443280966369564	24.036028805487717	29.05645411594303	22.464236112199696
13	29.172423467528507	19.749425364823786	25.5733616921899	25.504789475457805
14	23.095141437051225	20.341401940791897	25.18844427864518	31.3750123435117
15	24.838396479319684	28.007541628236282	22.248900375122343	24.905161517321687
16	25.360233709161236	25.899134561434856	24.463450031188387	24.27718169821552
17	23.63937594486421	26.12249280355367	25.706732958352696	24.531398293229422
18	24.40972899850066	25.0756918465989	26.857105236551227	23.657473918349208
19	25.2871788440738	25.198767889893876	25.464079130939133	24.04997413509319
20	25.40244306707032	24.81734206152103	25.18167832924118	24.598536542167476
21	26.320595226991816	24.566045632710836	24.514708637077224	24.598650503220124
22	25.467422873560064	25.029847177746507	25.006643593733465	24.49608635495996
23	24.052437686157706	24.831870199090766	25.66217275435021	25.453519360401316
24	24.473133078965766	25.386332498330518	25.540269813550875	24.600264609152845
25	24.287851724243335	25.14505526717366	25.608025124363877	24.95906788421913
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	121.0
1	121.0
2	348.0
3	575.0
4	575.0
5	575.0
6	1311.5
7	2048.0
8	2048.0
9	2048.0
10	2359.0
11	2670.0
12	2670.0
13	2670.0
14	3382.5
15	4095.0
16	4095.0
17	4095.0
18	6781.5
19	9468.0
20	9468.0
21	9468.0
22	15341.0
23	21214.0
24	21214.0
25	21214.0
26	31244.5
27	41275.0
28	41275.0
29	41275.0
30	51600.0
31	61925.0
32	61925.0
33	61925.0
34	75072.5
35	88220.0
36	88220.0
37	88220.0
38	98140.5
39	108061.0
40	108061.0
41	108061.0
42	121946.0
43	135831.0
44	135831.0
45	135831.0
46	151055.0
47	166279.0
48	166279.0
49	166279.0
50	166755.5
51	167232.0
52	167232.0
53	167232.0
54	153529.5
55	139827.0
56	139827.0
57	139827.0
58	127653.5
59	115480.0
60	115480.0
61	115480.0
62	102037.0
63	88594.0
64	88594.0
65	88594.0
66	73347.0
67	58100.0
68	58100.0
69	58100.0
70	44242.5
71	30385.0
72	30385.0
73	30385.0
74	23648.0
75	16911.0
76	16911.0
77	16911.0
78	14192.5
79	11474.0
80	11474.0
81	11474.0
82	7969.0
83	4464.0
84	4464.0
85	4464.0
86	3284.0
87	2104.0
88	2104.0
89	2104.0
90	1365.0
91	626.0
92	626.0
93	626.0
94	368.5
95	111.0
96	111.0
97	111.0
98	274.0
99	437.0
100	437.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007984175676913287
2	0.0034441542135704374
3	0.004853126391849252
4	0.01471593163980096
5	0.035067751992717174
6	0.04453917608003588
7	0.06622169237910432
8	0.08610385533926093
9	0.09627976551571904
10	0.11702296702926827
11	0.11647503340438206
12	0.1298602690980308
13	0.11741434818990128
14	0.12156298849261112
15	0.1103694872985072
16	0.11044776353063379
17	0.12132815979623132
18	0.12759025836635937
19	0.13048647895504362
20	0.11804055804691407
21	0.1286861256161318
22	0.1457503442197308
23	0.13518305288263965
24	0.13173889866906924
25	0.1279033632948658
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1277527.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.311858390488766
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.10344089694806	47.6281263644824
2	10.928518368434178	12.526673942991101
3	2.669153560705249	4.589224526808243
4	1.0680178860177576	2.4484035936783566
5	0.5531700904569826	1.5851602945062226
6	0.31649052457450405	1.088319607580729
7	0.21147197904533682	0.8483896481621925
8	0.14848999441708116	0.6808190025948979
9	0.1148407837228145	0.5923564860757222
>10	0.6806996241817608	7.504906623611229
>50	0.09729923444132511	3.893696229972263
>100	0.09693831097764218	11.448131537785956
>500	0.009011157632293867	3.4663711784451947
>1k	0.002457588445171055	1.6994209633055541
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1874	0.14668965900524997	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1473	0.11530088992248305	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1289	0.1008980632111885	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1287	0.10074151074693528	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.827623212660085E-5	0.0
2	0.0	0.0	0.0	7.827623212660085E-5	0.0
3	0.0	0.0	0.0	7.827623212660085E-5	0.0
4	0.0	0.0	0.0	7.827623212660085E-5	0.0
5	0.0	0.0	0.0	7.827623212660085E-5	0.0
6	0.0	0.0	0.0	7.827623212660085E-5	0.0
7	0.0	0.0	0.0	7.827623212660085E-5	0.0
8	0.0	0.0	0.0	7.827623212660085E-5	0.0
9	0.0	0.0	0.0	2.3482869637980253E-4	0.0
10	0.0	0.0	0.0	2.3482869637980253E-4	0.0
11	0.0	0.0	0.0	2.3482869637980253E-4	0.0
12	0.0	0.0	0.0	2.3482869637980253E-4	0.0
13	0.0	0.0	0.0	2.3482869637980253E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTTCT	275	0.0	15.89186	12
CGGTCGG	135	1.8189894E-12	13.370646	10
AAGACGG	245	0.0	13.182912	5
GCGGTCG	140	3.6379788E-12	12.892618	9
GGTTCTA	335	0.0	12.4788475	13
GCGTAAC	130	1.9826984E-10	12.422846	11
CGAACGA	115	5.298716E-9	12.394475	16
CGCGCTA	100	1.4368197E-7	12.35316	16
GCGCGCT	100	1.4382931E-7	12.352192	15
GACTTAG	55	0.0030683544	12.090685	7
CTATTTT	375	0.0	11.91018	17
CAAGACG	265	0.0	11.828578	4
CGCAAGA	290	0.0	11.78734	2
ACCCGCA	65	8.02294E-4	11.692091	7
CGCGTAT	65	8.02294E-4	11.692091	7
AACGGAC	90	7.442799E-6	11.613172	15
CGCGTAA	140	6.7848305E-10	11.535953	10
GGTCGGC	165	7.2759576E-12	11.514937	11
CGGTCCA	215	0.0	11.488609	10
GTCCTAA	365	0.0	11.446459	1
>>END_MODULE