FastQCFastQC Report
Thu 2 Feb 2017
SRR4062340_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062340_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1277527
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13030.1019939304609609No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT12990.1016808255324545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG5850.021.8118694
GTATCAA18250.020.37571
TAGACCG550.00448386519.999915
TGAACCG550.00448386519.999915
ACCGTTA550.00448386519.999918
TGTAGGA8300.019.6151392
AGGACGT6300.019.5554685
GGACGTG6450.019.1006896
GCGAGAA4400.018.99991420
TAACGGC2250.018.57769436
GCATATA1901.8189894E-1218.5291351
GTAGGAC8000.018.424923
TAAGGCG600.007412516518.3332525
GACGTGA3500.018.228497
ATAATAC1154.273448E-617.2173163
TTAACGG2450.017.0611535
CGGTCCA4600.016.73905810
TTAGGAC4750.016.673613
AATCACG3700.016.64857334
TATGGCG7400.016.64857316
Produced by FastQC (version 0.11.5)