##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062340_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1277527
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.1549070978539	32.0	32.0	32.0	32.0	32.0
2	31.068937094871576	32.0	32.0	32.0	32.0	32.0
3	31.294477533547237	32.0	32.0	32.0	32.0	32.0
4	31.475549244751775	32.0	32.0	32.0	32.0	32.0
5	31.334809362150466	32.0	32.0	32.0	32.0	32.0
6	34.92566810721026	36.0	36.0	36.0	36.0	36.0
7	34.948838654682056	36.0	36.0	36.0	36.0	36.0
8	34.85915444448532	36.0	36.0	36.0	32.0	36.0
9	35.06856058619505	36.0	36.0	36.0	36.0	36.0
10	34.87165594151826	36.0	36.0	36.0	32.0	36.0
11	35.06987249584549	36.0	36.0	36.0	36.0	36.0
12	34.95551874833174	36.0	36.0	36.0	32.0	36.0
13	35.01728965415212	36.0	36.0	36.0	36.0	36.0
14	34.97229256211415	36.0	36.0	36.0	32.0	36.0
15	34.904530393486795	36.0	36.0	36.0	32.0	36.0
16	34.93950891057489	36.0	36.0	36.0	32.0	36.0
17	34.90744540037119	36.0	36.0	36.0	32.0	36.0
18	34.926693525851114	36.0	36.0	36.0	32.0	36.0
19	34.902516346034176	36.0	36.0	36.0	32.0	36.0
20	34.898809183680655	36.0	36.0	36.0	32.0	36.0
21	34.90516521372934	36.0	36.0	36.0	32.0	36.0
22	34.87985772512049	36.0	36.0	36.0	32.0	36.0
23	34.83466181145291	36.0	36.0	36.0	32.0	36.0
24	34.819036309995795	36.0	36.0	36.0	32.0	36.0
25	34.78385192641721	36.0	36.0	36.0	32.0	36.0
26	34.73149060646077	36.0	36.0	36.0	32.0	36.0
27	34.71387610594532	36.0	36.0	36.0	32.0	36.0
28	34.69354776846204	36.0	36.0	36.0	32.0	36.0
29	34.669535751494884	36.0	36.0	36.0	32.0	36.0
30	34.65079563876145	36.0	36.0	36.0	32.0	36.0
31	34.65188054733873	36.0	36.0	36.0	32.0	36.0
32	34.61521830849759	36.0	36.0	36.0	32.0	36.0
33	34.5978785575569	36.0	36.0	36.0	32.0	36.0
34	34.582359511775486	36.0	36.0	36.0	32.0	36.0
35	34.569265463665346	36.0	36.0	36.0	32.0	36.0
36	34.52380419357086	36.0	36.0	36.0	32.0	36.0
37	34.51705286854994	36.0	36.0	36.0	32.0	36.0
38	34.488222949495395	36.0	36.0	36.0	32.0	36.0
39	34.46793531565282	36.0	36.0	36.0	32.0	36.0
40	34.471715274902216	36.0	36.0	36.0	32.0	36.0
41	34.43594147129571	36.0	36.0	36.0	32.0	36.0
42	34.407227401064716	36.0	36.0	36.0	32.0	36.0
43	34.394207715375096	36.0	36.0	36.0	32.0	36.0
44	34.35377569319474	36.0	36.0	36.0	32.0	36.0
45	34.32159320311821	36.0	36.0	36.0	32.0	36.0
46	34.31948209313776	36.0	36.0	36.0	32.0	36.0
47	34.30408280999149	36.0	36.0	36.0	32.0	36.0
48	34.25467954884711	36.0	36.0	36.0	32.0	36.0
49	34.23250858885957	36.0	36.0	36.0	32.0	36.0
50	33.66017861070647	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	4.0
21	29.0
22	83.0
23	288.0
24	766.0
25	1851.0
26	3753.0
27	7401.0
28	12943.0
29	20896.0
30	31925.0
31	48267.0
32	75669.0
33	130855.0
34	280702.0
35	662092.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.845511779709184	18.588955710422855	12.059660269648637	26.505872240219325
2	16.05419736841075	20.6206129415826	37.159358392584146	26.165831297422503
3	18.327967480820305	24.253418529451135	28.975681846823893	28.44293214290467
4	12.483659823753097	16.279042461758117	36.48004032794636	34.75725738654243
5	14.624829553371125	36.505636675887615	33.41865449313752	15.450879277603743
6	33.98323632276185	36.06335380000689	16.667945181460386	13.285464695770882
7	30.010011530088992	30.618530958641188	21.034232544595927	18.337224966673894
8	27.73530422448997	33.533303014339424	19.499392185057538	19.232000576113066
9	27.523175635426885	14.097079748608051	18.82206794846606	39.557676667499
10	15.935083955172768	26.83927619533677	31.71189336898555	25.513746480504913
11	37.50574351853229	21.0182641932421	22.564298053974593	18.911694234251016
12	24.642923398096478	23.792217307344583	29.001813660298374	22.56304563426057
13	29.582153645284993	19.663224338898512	25.246041766631937	25.508580249184558
14	23.207963510751632	20.236597739225864	25.01371790968019	31.541720840342318
15	24.942799643373487	28.095609720968717	22.042430414386548	24.91916022127125
16	25.495116737258783	25.92931499686504	24.305709390095082	24.2698588757811
17	23.82564125846264	26.116395191647612	25.42740779646927	24.630555753420474
18	24.823741494308926	24.93418925783956	26.64209836660986	23.59997088124165
19	25.520556512699926	25.077513038863366	25.353123652181125	24.04880679625558
20	25.548657680033376	24.7283227673466	25.081974784094584	24.64104476852544
21	26.704249694918385	24.397136029218952	24.343438533980102	24.55517574188256
22	25.741530315993323	25.041505972085133	24.84409331466184	24.372870397259703
23	24.28911482888424	24.70460506901224	25.587560967400297	25.41871913470322
24	24.698656075370618	25.272499133090733	25.403455269438535	24.625389522100118
25	24.64636755231005	24.915481238361302	25.541299714213476	24.896851495115172
26	24.122856111847344	25.796793335874703	26.006260533045484	24.074090019232468
27	25.192970481250104	25.196258082999417	24.97293599274223	24.63783544300825
28	24.084579034337438	25.235083094134215	25.77338874246885	24.9069491290595
29	24.406529177074145	24.998532320647627	25.552884596568216	25.042053905710016
30	24.20700306138344	25.29097232387261	25.984656292978542	24.517368321765414
31	24.98303362668656	25.00902133575259	24.877282437083522	25.130662600477326
32	24.588443140536363	25.29245957228301	24.92041264098528	25.198684646195346
33	24.169039088802037	25.04823772804802	25.56055566731662	25.222167515833327
34	24.8321953273786	25.06107503011678	25.95498960100256	24.15174004150206
35	25.44682030203667	24.911645702987098	25.48940257231354	24.15213142266269
36	24.16786494532014	25.4007156013141	25.3689354510707	25.06248400229506
37	25.20205052417679	25.23007341527811	24.81184350702568	24.756032553519418
38	24.432360333675923	25.12573119785335	25.503648846560584	24.938259621910145
39	25.08314892757648	24.697560208120848	25.04823772804802	25.171053136254656
40	25.51742546341486	24.844641248286727	25.598284811201644	24.03964847709677
41	24.29201104947293	25.09950866009094	26.127901797770225	24.48057849266591
42	25.438679573895502	25.508893354113066	25.49175085927734	23.560676212714096
43	24.390404273256063	24.570909264540006	25.8262251991543	25.212461263049622
44	23.96356397946971	25.23539619906272	25.491907411741593	25.309132409725976
45	24.271267847959376	25.415901190346663	25.550771138300792	24.762059823393166
46	24.150957279180794	24.86757618429982	25.669985839829607	25.311480696689774
47	24.687619126640765	24.552984007383014	25.91608631363564	24.84331055234058
48	25.018727588536287	25.479618043297712	25.020527941875205	24.481126426290796
49	24.181250181013787	25.404707689152556	25.430851950682843	24.983190179150814
50	24.05248577916553	25.77800704016432	25.148039924009435	25.021467256660717
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9.0
1	12.0
2	15.0
3	46.0
4	77.0
5	90.0
6	103.0
7	123.5
8	144.0
9	172.0
10	200.0
11	301.0
12	402.0
13	767.5
14	1133.0
15	1509.5
16	1886.0
17	2280.5
18	2675.0
19	3048.0
20	3421.0
21	4192.0
22	4963.0
23	5556.5
24	6150.0
25	7823.5
26	9497.0
27	12191.5
28	14886.0
29	17497.0
30	20108.0
31	22137.0
32	24166.0
33	28331.5
34	32497.0
35	37905.0
36	43313.0
37	49504.0
38	55695.0
39	58041.0
40	60387.0
41	64306.5
42	68226.0
43	68963.5
44	69701.0
45	75524.0
46	81347.0
47	86008.5
48	90670.0
49	93439.5
50	96209.0
51	92158.5
52	88108.0
53	86038.0
54	83968.0
55	85084.5
56	86201.0
57	84243.5
58	82286.0
59	74360.0
60	66434.0
61	58426.0
62	50418.0
63	44177.5
64	37937.0
65	32736.0
66	27535.0
67	23845.0
68	20155.0
69	18559.0
70	16963.0
71	13315.0
72	9667.0
73	8719.5
74	7772.0
75	5755.0
76	3738.0
77	3363.0
78	2988.0
79	2607.5
80	2227.0
81	1789.0
82	1351.0
83	1102.5
84	854.0
85	726.5
86	599.0
87	445.5
88	292.0
89	195.0
90	98.0
91	62.0
92	26.0
93	23.0
94	20.0
95	12.5
96	5.0
97	4.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012602473372382735
2	0.003757259142076841
3	3.1310492850640336E-4
4	7.827623212660084E-5
5	7.827623212660084E-5
6	2.3482869637980256E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1277527.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.24721978206066
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.9717670626434	51.43037272773723
2	10.589619070524	12.971694532413688
3	2.5382653636185664	4.6638508977221536
4	0.9665680311615252	2.3679841855545436
5	0.49830620849922475	1.525993493535868
6	0.28065371022702346	1.0313555683755162
7	0.1795917078482878	0.7699644961133798
8	0.13900949280305078	0.6811155966000985
9	0.09388878455631434	0.5175384320512014
>10	0.5749779570900416	6.825894525630982
>50	0.07929660840181986	3.3669717061327034
>100	0.07984088047047508	9.998612093763146
>500	0.0066747867520163305	2.721588145740676
>1k	0.0015403354043114607	1.1270635986289887
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1303	0.1019939304609609	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1299	0.1016808255324545	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	7.827623212660085E-5
13	0.0	0.0	0.0	0.0	1.565524642532017E-4
14	0.0	0.0	0.0	0.0	1.565524642532017E-4
15	0.0	0.0	0.0	0.0	3.131049285064034E-4
16	0.0	0.0	0.0	7.827623212660085E-5	3.131049285064034E-4
17	0.0	0.0	0.0	7.827623212660085E-5	3.131049285064034E-4
18	0.0	0.0	0.0	1.565524642532017E-4	3.131049285064034E-4
19	0.0	0.0	0.0	1.565524642532017E-4	3.131049285064034E-4
20	0.0	0.0	0.0	1.565524642532017E-4	3.131049285064034E-4
21	0.0	0.0	0.0	1.565524642532017E-4	3.131049285064034E-4
22	0.0	0.0	0.0	3.131049285064034E-4	3.131049285064034E-4
23	0.0	0.0	0.0	6.262098570128068E-4	3.131049285064034E-4
24	0.0	0.0	0.0	0.0010958672497724119	3.131049285064034E-4
25	0.0	0.0	0.0	0.0011741434818990128	3.131049285064034E-4
26	0.0	0.0	0.0	0.0012524197140256136	3.131049285064034E-4
27	0.0	0.0	0.0	0.0018786295710384203	3.131049285064034E-4
28	0.0	0.0	0.0	0.004226916534836446	3.131049285064034E-4
29	0.0	0.0	0.0	0.009862805247951707	3.131049285064034E-4
30	0.0	0.0	0.0	0.020664925281422622	3.131049285064034E-4
31	0.0	0.0	0.0	0.055106467417127	3.131049285064034E-4
32	0.0	0.0	0.0	0.09447941217680722	3.131049285064034E-4
33	0.0	0.0	0.0	0.13620064390028547	3.131049285064034E-4
34	0.0	0.0	0.0	0.1853581176757908	4.6965739275960506E-4
35	0.0	0.0	0.0	0.2399949277001582	4.6965739275960506E-4
36	0.0	0.0	0.0	0.32163703780820285	4.6965739275960506E-4
37	0.0	0.0	0.0	0.4409300155691426	4.6965739275960506E-4
38	0.0	0.0	0.0	0.6041359595531053	4.6965739275960506E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACG	585	0.0	21.811869	4
GTATCAA	1825	0.0	20.3757	1
TAGACCG	55	0.004483865	19.99991	5
TGAACCG	55	0.004483865	19.99991	5
ACCGTTA	55	0.004483865	19.99991	8
TGTAGGA	830	0.0	19.615139	2
AGGACGT	630	0.0	19.555468	5
GGACGTG	645	0.0	19.100689	6
GCGAGAA	440	0.0	18.999914	20
TAACGGC	225	0.0	18.577694	36
GCATATA	190	1.8189894E-12	18.529135	1
GTAGGAC	800	0.0	18.42492	3
TAAGGCG	60	0.0074125165	18.333252	5
GACGTGA	350	0.0	18.22849	7
ATAATAC	115	4.273448E-6	17.217316	3
TTAACGG	245	0.0	17.06115	35
CGGTCCA	460	0.0	16.739058	10
TTAGGAC	475	0.0	16.67361	3
AATCACG	370	0.0	16.648573	34
TATGGCG	740	0.0	16.648573	16
>>END_MODULE