FastQCFastQC Report
Thu 2 Feb 2017
SRR4062339_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062339_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1283400
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG32630.2542465326476547No Hit
TCCATGTACTCTGCGTTGATACCAC27270.21248246844319776No Hit
GTATCAACGCAGAGTACTTTTTTTT26310.20500233754090694No Hit
TATCAACGCAGAGTACTTTTTTTTT19450.1515505688016207No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18350.14297958547607917No Hit
GTCCTAAAGTGTGTATTTCTCATTT17800.13869409381330838No Hit
GTCCTACAGTGGACATTTCTAAATT16130.12568178276453174No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG14320.11157861929250429No Hit
CTTTAGGACGTGAAATATGGCGAGG14170.11040984883902134No Hit
GAATAGGACCGCGGTTCTATTTTGT14090.1097865045971638No Hit
CTATTGGAGCTGGAATTACCGCGGC13800.10752688172043011No Hit
CTGTAGGACGTGGAATATGGCAAGA13730.10698145550880475No Hit
GTCTTGCGCCGGTCCAAGAATTTCA13400.10441016051114227No Hit
GATTAAGAGGGACGGCCGGGGGCAT13300.10363098020882032No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAAG402.775031E-416.6136761
AACCGCG1350.015.48300657
GACCGAG400.005274986414.2514047
CTCGTAG1006.5847416E-1014.25084910
TACGGCC400.005285918214.2469584
TCGCCAG1750.014.1178817
TTAACCA1750.014.1112724
GCTCGTA1152.5465852E-1114.0443139
GAACCGC2100.013.5727676
GGACCGA655.4459968E-513.1551426
ATCGCCA2050.012.97840816
TAACCAG2200.012.5210315
GTCCTAG1600.012.4602571
CGCGGTC1457.2759576E-1212.44901810
TATTAGC1001.4508078E-712.3439942
GCGTTAT1001.4545003E-712.3415881
CGTCGTA1700.012.29484910
ACGAAAT701.0892132E-412.21596612
AGAACCG2100.012.2126325
CTTACAC701.0943814E-412.2097733
Produced by FastQC (version 0.11.5)