##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062339_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1283400
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16259700794764	32.0	32.0	32.0	32.0	32.0
2	30.822953872526103	32.0	32.0	32.0	32.0	32.0
3	30.818353592021193	32.0	32.0	32.0	32.0	32.0
4	30.87605734767025	32.0	32.0	32.0	32.0	32.0
5	30.67817749727287	32.0	32.0	32.0	32.0	32.0
6	34.31528907589216	36.0	36.0	36.0	32.0	36.0
7	34.19486052672588	36.0	36.0	36.0	32.0	36.0
8	34.19215599189653	36.0	36.0	36.0	32.0	36.0
9	34.34913822658563	36.0	36.0	36.0	32.0	36.0
10	34.027695963846035	36.0	36.0	36.0	32.0	36.0
11	34.34343384759233	36.0	36.0	36.0	32.0	36.0
12	34.12112124045504	36.0	36.0	36.0	32.0	36.0
13	34.23756895745676	36.0	36.0	36.0	32.0	36.0
14	34.150853202431044	36.0	36.0	36.0	32.0	36.0
15	34.0834572230014	36.0	36.0	36.0	32.0	36.0
16	34.09065061555244	36.0	36.0	36.0	32.0	36.0
17	34.011848215677105	36.0	36.0	36.0	32.0	36.0
18	34.03383045036622	36.0	36.0	36.0	32.0	36.0
19	34.001661991584854	36.0	36.0	36.0	32.0	36.0
20	33.989759233286584	36.0	36.0	36.0	32.0	36.0
21	33.95654667290011	36.0	36.0	36.0	32.0	36.0
22	33.900070126227206	36.0	36.0	36.0	32.0	36.0
23	33.87183964469378	36.0	36.0	36.0	32.0	36.0
24	33.84304971170329	36.0	36.0	36.0	32.0	36.0
25	33.367794919744426	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	9.0
4	60.0
5	127.0
6	216.0
7	55.0
8	170.0
9	239.0
10	141.0
11	40.0
12	90.0
13	67.0
14	218.0
15	266.0
16	438.0
17	605.0
18	780.0
19	1099.0
20	1748.0
21	2599.0
22	3898.0
23	5846.0
24	7862.0
25	11215.0
26	15486.0
27	19753.0
28	26850.0
29	35778.0
30	46451.0
31	63491.0
32	91955.0
33	131537.0
34	289849.0
35	524462.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.08292786320097	17.632573927517214	11.358436636005747	25.926061573276066
2	16.09718011234668	20.135286660136874	38.28958451676044	25.477948710756
3	18.99731480658971	24.49767558562307	28.302920362119295	28.20208924566792
4	12.283653565152337	15.579515034854676	36.1535554109501	35.98327598904289
5	14.179174279318321	37.121186139465856	33.81833459084985	14.881304990365967
6	33.84805950649274	35.734687227650376	17.177962891072625	13.239290374784263
7	29.80858296817253	30.41706301557846	21.27777691144136	18.496577104807645
8	27.764641290717044	33.82002458152144	19.69734781605036	18.71798631171116
9	27.5493889265854	14.071612968639105	18.682000046795665	39.69699805797983
10	15.932096819993072	27.02069766063076	31.809508504669687	25.237697014706477
11	37.48908826665897	20.799811526378896	22.655137739424298	19.05596246753784
12	24.687158417718706	23.60119495770914	29.55288951790525	22.158757106666908
13	29.94131937022469	19.721434734376924	25.318874068341636	25.018371827056747
14	23.108926725567063	20.405593587268154	25.15085912663585	31.33462056052893
15	24.95237798076398	28.520714213281124	22.329149668088956	24.19775813786594
16	25.178360533997317	25.59569220112422	25.205506593840344	24.020440671038116
17	23.36552931456964	26.123836338005535	26.106517443325476	24.40411690409935
18	24.286147847111316	24.674135142536375	27.97549898848677	23.06421802186554
19	25.51550393342628	25.05440010860516	25.201391581695347	24.228704376273207
20	25.348631840407904	24.70191938639548	25.83136485191923	24.118083921277385
21	26.37406533597973	24.458862300116092	24.07688274725686	25.09018961664732
22	25.343064918768626	25.485714374898066	24.869621674601607	24.301599031731698
23	23.783537049246842	25.053429098669245	25.67280870471011	25.490225147373803
24	24.64217138478991	25.5397370188868	25.586784313511313	24.231307282811976
25	24.511732235854026	25.124500185297162	25.722288322378045	24.64147925647077
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	190.0
1	190.0
2	462.5
3	735.0
4	735.0
5	735.0
6	1988.0
7	3241.0
8	3241.0
9	3241.0
10	3250.5
11	3260.0
12	3260.0
13	3260.0
14	3496.5
15	3733.0
16	3733.0
17	3733.0
18	6084.5
19	8436.0
20	8436.0
21	8436.0
22	14623.5
23	20811.0
24	20811.0
25	20811.0
26	32438.0
27	44065.0
28	44065.0
29	44065.0
30	56368.5
31	68672.0
32	68672.0
33	68672.0
34	79318.5
35	89965.0
36	89965.0
37	89965.0
38	98300.5
39	106636.0
40	106636.0
41	106636.0
42	119826.0
43	133016.0
44	133016.0
45	133016.0
46	152517.0
47	172018.0
48	172018.0
49	172018.0
50	167441.0
51	162864.0
52	162864.0
53	162864.0
54	148534.0
55	134204.0
56	134204.0
57	134204.0
58	123815.0
59	113426.0
60	113426.0
61	113426.0
62	101385.5
63	89345.0
64	89345.0
65	89345.0
66	74990.0
67	60635.0
68	60635.0
69	60635.0
70	45878.0
71	31121.0
72	31121.0
73	31121.0
74	24376.5
75	17632.0
76	17632.0
77	17632.0
78	14527.5
79	11423.0
80	11423.0
81	11423.0
82	7923.0
83	4423.0
84	4423.0
85	4423.0
86	3274.0
87	2125.0
88	2125.0
89	2125.0
90	1414.0
91	703.0
92	703.0
93	703.0
94	424.0
95	145.0
96	145.0
97	145.0
98	360.5
99	576.0
100	576.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009038491506934705
2	0.003973819541841982
3	0.005142589995324918
4	0.016752376499922082
5	0.03490727754402369
6	0.04534829359513791
7	0.06700950599968833
8	0.08851488234377436
9	0.09583917718560074
10	0.11812373383200873
11	0.11866916004363409
12	0.13098020882032102
13	0.12046127473897458
14	0.12272089761570827
15	0.1114227832320399
16	0.11266947171575503
17	0.12178588125292193
18	0.1291101760947483
19	0.13191522518310736
20	0.11937042231572387
21	0.12903225806451613
22	0.15092722455976312
23	0.13986286426679134
24	0.1333177497272869
25	0.13051270063892786
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1283400.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.6535083907149
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.86676873544835	37.39837977637278
2	13.60180126838541	13.235507042804523
3	4.368702587976414	6.376581240619451
4	1.7925514706122643	3.488556720648886
5	0.8914137444112677	2.16852030466561
6	0.5157188698649369	1.5054919417334227
7	0.35438262734732684	1.206937069321673
8	0.23488481608840203	0.9142376296326875
9	0.16342615747239275	0.7156130331460819
>10	0.9565592973257375	8.739958574512999
>50	0.11272284172846223	3.824753985517619
>100	0.12309310747874412	12.565780470918387
>500	0.012838911328747581	4.249158507354026
>1k	0.005135564531499032	3.610523702751799
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	3263	0.2542465326476547	No Hit
TCCATGTACTCTGCGTTGATACCAC	2727	0.21248246844319776	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2631	0.20500233754090694	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1945	0.1515505688016207	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1835	0.14297958547607917	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1780	0.13869409381330838	No Hit
GTCCTACAGTGGACATTTCTAAATT	1613	0.12568178276453174	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1432	0.11157861929250429	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1417	0.11040984883902134	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1409	0.1097865045971638	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1380	0.10752688172043011	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1373	0.10698145550880475	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1340	0.10441016051114227	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1330	0.10363098020882032	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.116721209287829E-4
2	0.0	0.0	0.0	0.0	3.116721209287829E-4
3	0.0	0.0	0.0	0.0	3.116721209287829E-4
4	0.0	0.0	0.0	0.0	3.116721209287829E-4
5	0.0	0.0	0.0	0.0	3.116721209287829E-4
6	0.0	0.0	0.0	0.0	3.8959015116097863E-4
7	0.0	0.0	0.0	7.791803023219573E-5	3.8959015116097863E-4
8	0.0	0.0	0.0	7.791803023219573E-5	3.8959015116097863E-4
9	0.0	0.0	0.0	1.5583606046439146E-4	3.8959015116097863E-4
10	0.0	0.0	0.0	1.5583606046439146E-4	4.675081813931744E-4
11	0.0	0.0	0.0	2.337540906965872E-4	4.675081813931744E-4
12	0.0	0.0	0.0	3.8959015116097863E-4	4.675081813931744E-4
13	0.0	0.0	0.0	3.8959015116097863E-4	7.012622720897616E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTAAG	40	2.775031E-4	16.613676	1
AACCGCG	135	0.0	15.4830065	7
GACCGAG	40	0.0052749864	14.251404	7
CTCGTAG	100	6.5847416E-10	14.250849	10
TACGGCC	40	0.0052859182	14.246958	4
TCGCCAG	175	0.0	14.11788	17
TTAACCA	175	0.0	14.111272	4
GCTCGTA	115	2.5465852E-11	14.044313	9
GAACCGC	210	0.0	13.572767	6
GGACCGA	65	5.4459968E-5	13.155142	6
ATCGCCA	205	0.0	12.978408	16
TAACCAG	220	0.0	12.521031	5
GTCCTAG	160	0.0	12.460257	1
CGCGGTC	145	7.2759576E-12	12.449018	10
TATTAGC	100	1.4508078E-7	12.343994	2
GCGTTAT	100	1.4545003E-7	12.341588	1
CGTCGTA	170	0.0	12.294849	10
ACGAAAT	70	1.0892132E-4	12.215966	12
AGAACCG	210	0.0	12.212632	5
CTTACAC	70	1.0943814E-4	12.209773	3
>>END_MODULE