FastQCFastQC Report
Thu 2 Feb 2017
SRR4062339_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062339_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1283400
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14890.11601994701573944No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC14010.10916316035530621No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13860.10799438990182328No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA13720.10690353747857254No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA13630.10620227520648279No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGAC6650.020.1803633
GACGTGA5700.019.6841227
TAGGACG10700.019.5326234
ATAGACC808.989426E-519.2499163
GGACGTG10550.018.976226
TGTAGGA11200.018.8577942
AGGACGT11100.018.432355
GGCGTTA3350.018.38797842
TGTGCGA600.007412533718.33325210
TCGTATA600.007412533718.33325244
AGTGTAT4450.018.29205510
GGACCGT1102.7968163E-617.999926
ATACCGT3550.017.971756
TAGGACC9750.017.8255634
TTAGTAC1254.853282E-717.5999223
CTGTAGG11800.017.5280781
CGGCGTT3550.017.35203641
TAGGACA3300.017.3332564
AGGACCT9150.017.3113985
TAGAACA8800.017.2499244
Produced by FastQC (version 0.11.5)