FastQCFastQC Report
Thu 2 Feb 2017
SRR4062338_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062338_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2232696
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG103750.46468484737734117No Hit
TCCATGTACTCTGCGTTGATACCAC84860.38007861347895106No Hit
GTATCAACGCAGAGTACTTTTTTTT53570.23993414240004013No Hit
GAGTACATGGAAGCAGTGGTATCAA38540.1726164242691347No Hit
GTACTTTTTTTTTTTTTTTTTTTTT36450.16325554397015984No Hit
TATCAACGCAGAGTACTTTTTTTTT36040.1614191990311265No Hit
GTCCTAAAGTGTGTATTTCTCATTT32760.1467284395188597No Hit
GTCCTACAGTGGACATTTCTAAATT32230.14435462776840197No Hit
CATGTACTCTGCGTTGATACCACTG31810.14247349392841657No Hit
GTCCTACAGTGTGCATTTCTCATTT28290.12670780079330102No Hit
CTGTAGGACGTGGAATATGGCAAGA27470.12303511091523432No Hit
CTTTAGGACGTGAAATATGGCGAGG27050.12115397707524894No Hit
CCCATGTACTCTGCGTTGATACCAC25860.11582409786195702No Hit
GCTTCCATGTACTCTGCGTTGATAC25100.11242014138960253No Hit
GTACATGGGAAGCAGTGGTATCAAC24710.11067337425247324No Hit
GAGTACTTTTTTTTTTTTTTTTTTT24070.10780688459154314No Hit
CTGTAGGACCTGGAATATGGCGAGA23680.10606011745441385No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAA307.764675E-418.9852281
CTCGACG1000.017.1020413
TCGACGC1100.015.54730814
ACGCTTA1153.765308E-1013.22015417
CGTGCGC801.9973322E-613.06435110
AATATCG751.477453E-512.66817814
GACGCTT1301.9826984E-1012.42511616
ACGGTAT3900.012.1814869
CGAATTG550.003066425212.09235114
CGAACGA1603.6379788E-1211.87694816
CGCAATA1850.011.8032162
CGCATCG2500.011.781404513
AGGACCT14750.011.2061055
CCAACGA1701.0913936E-1111.17880619
TAACGAA1701.0913936E-1111.17780313
TGTAGGA21450.011.1536632
CGACGCT1451.2169039E-911.13900915
CGGTCCA3500.011.13014910
TGCGCTA600.005870821411.08490410
CGACTCG600.00587180211.08465514
Produced by FastQC (version 0.11.5)